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Vol. 19, Issue 5, 2241-2250, May 2008

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The Mood Stabilizer Valproate Inhibits both Inositol- and Diacylglycerol-signaling Pathways in Caenorhabditis elegans

Suzumi M. Tokuoka^*,, Adolfo Saiardi, and Stephen J. Nurrish

MRC Cell Biology Unit, MRC Laboratory for Molecular Cell Biology, Departments of Neuroscience, Physiology, and Pharmacology and Cell and Developmental Biology, University College London, London WC1E 6BT, United Kingdom

Submitted September 28, 2007; Revised February 5, 2008; Accepted February 13, 2008
Monitoring Editor: Thomas F. J. Martin

	ABSTRACT

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES

 
The antiepileptic valproate (VPA) is widely used in the treatment of bipolar disorder, although the mechanism of its action in the disorder is unclear. We show here that VPA inhibits both inositol phosphate and diacylglycerol (DAG) signaling in Caenorhabditis elegans. VPA disrupts two behaviors regulated by the inositol-1,4,5-trisphosphate (IP₃): defecation and ovulation. VPA also inhibits two activities regulated by DAG signaling: acetylcholine release and egg laying. The effects of VPA on DAG signaling are relieved by phorbol ester, a DAG analogue, suggesting that VPA acts to inhibit DAG production. VPA reduces levels of DAG and inositol-1-phosphate, but phosphatidylinositol-4,5-bisphosphate (PIP₂) is slightly increased, suggesting that phospholipase C-mediated hydrolysis of PIP₂ to form DAG and IP₃ is defective in the presence of VPA.

	INTRODUCTION

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES

 
Bipolar disorder is a prevalent and devastating illness, which is characterized by mood swings between depression and mania. VPA reduces mood swings in bipolar patients, although its mechanism of action is controversial (Harwood, 2005). Several lines of evidence suggest that VPA interferes with inositol phosphate signaling. For example, it decreases myoinositol levels in the mouse and rat brain (O'Donnell et al., 2000; Shaltiel et al., 2004) and inositol-1,4,5-trisphosphate [Ins(1,4,5)P₃, henceforth referred to as IP₃] in Dictyostelium (Williams et al., 2002). Moreover, another bipolar treatment, lithium (Li⁺), is known to alter inositol signaling by inhibition of two enzymes critical for inositol recycling: inositol-1(or 4)-monophosphatase (IMPase) (Allison et al., 1980; Hallcher and Sherman, 1980) and inositol-1,4-bisphosphate 1-phosphatase (IPPase) (Inhorn and Majerus, 1987). Thus, it has been suggested that bipolar disorder is associated with defective neuronal inositol phosphate signaling, which can be alleviated by drugs that modulate these signaling pathways.

Inositol is essential for the production of phosphatidylinositol-4,5-bisphosphate [PI(4,5)₂P, henceforth referred to as PIP₂], which is a signaling molecule in its own right. PIP₂ can give rise to many other molecules that alter neuronal activity. For example, PIP₂ can be hydrolyzed by phospholipase C (PLC) to DAG and IP₃; DAG activates neuroregulators such as UNC-13 and protein kinase C (PKC), IP₃ binds to the IP₃ receptor (IP₃R), triggering the release of Ca²⁺ from the ER. Both DAG and Ca²⁺ are important regulators of synaptic efficacy and plasticity. IP₃ can be both phosphorylated and dephosphorylated on multiple positions to give rise to other inositol phosphates, some of which have been implicated in the control of vesicle fusion and recycling (De Camilli et al., 1996; McPherson et al., 1996; Fukuda and Mikoshiba, 1997; Acharya et al., 1998). PIP₂ can also be phosphorylated to PIP₃, which has been implicated in control of synaptic plasticity and learning and memory (Raymond et al., 2002; Horwood et al., 2006; Karpova et al., 2006). Thus, changes in inositol signaling by drugs such as VPA would be expected to alter neuronal function. However, the role of inositol signaling in bipolar disorder and its treatment remain controversial. VPA has other targets that are unrelated to inositol signaling; it potentiates GABAergic neurotransmission (Loscher, 1999), for example, and it inhibits histone deacetylase transcriptional repressors. Thus, VPA could potentially alter levels of transcription via multiple routes (Phiel et al., 2001).

To better understand VPA action, we have taken advantage of Caenorhabditis elegans as a genetic model system. C. elegans has a simple nervous system, but it possesses many of the components involved in mammalian synaptic transmission. By combining behavioral assays and genetics, we describe for the first time that VPA alters C. elegans behaviors in two ways. First, VPA alters inositol phosphate-regulated behaviors in C. elegans. Second, we show that VPA inhibits DAG-regulated behaviors most likely by inhibiting DAG signaling via UNC-13. This is the first report of an effect of VPA on DAG signaling.

	MATERIALS AND METHODS

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES

 
Strains
All strains were cultivated at 20°C, and they were maintained as described previously (Brenner, 1974). The wild-type N2, egl-8(md1971), pkc-1(nj3) (Okochi et al., 2005), itr-1(sy327)unc-24(e138), and unc-24(e138) strains were obtained from the Caenorhabditis Genetics Center (University of Minnesota, Minneapolis, MN). The gain-of-function itr-1(sy327) mutation is only available marked with the closely linked unc-24(e138) mutation as itr-1(sy327) has no obvious visible phenotype. We have been unable to obtain recombinants carrying only the itr-1(sy327) mutation. Other strains have been described previously: egl-8(md1971) (Lackner et al., 1999; Miller et al., 1999) and unc-13(s69);nuIs46(UNC-13(+)(Sieburth et al., 2006). QT181 unc-13(s69); nzIs25 expresses UNC-13(H173K)::GFP in a putative null unc-13(s69) background (McMullan et al., 2006). KP1683 nuIs59 expresses rol-6(gf), UNC-13::YFP and p.acr-2::CFP::SNB-1 (Nurrish et al., 1999). The strain expressing p.rab-3:: PHPLC-1::GFP as an extrachromosomal array also containing the rol-6 marker was a gift from G. Lesa (MRC Laboratory for Molecular Cell Biology, London, United Kingdom), and it is described in Marza et al. (2007).

Preparation of Assay Plates
Assay plates for all the experiments were made 1 day before the assays. NaCl-, VPA-, or LiCl-containing plates were made by adding the drug stock solutions (dissolved in water) to melted nematode growth medium (NGM) agar with standard supplements (Brenner, 1974). Standard food stocks were made by growing OP50 in Luria Broth (OD₆₀₀ of 0.2–0.3) and then centrifuging the bacteria to make a 100-fold concentrated bacterial suspension. Suspension (100 µl) was seeded to each drug-containing NGM agar plate of 55 mm in diameter. The suspension usually spread to 30 mm in diameter. Seeded plates were allowed to dry for 1–2 h before use in assays.

Defecation Assay
First-day adult animals (22–24 h after larval [L]4 stage) were transferred onto drug-containing seeded plates, and then the posterior body contraction (pBoc) and enteric muscle contractions (EMCs) were recorded using the Etho program (obtained from Jim Thomas, University of Washington, Seattle, WA) between 60 and 120 min after the transfer. For each drug concentration, a minimum of eight different animals were scored for 5 min from the first posterior body contraction. If the animal moved off from bacterial lawn during the observation, it was not recorded.

Analysis of Brood Size, Sheath Contraction, and Ovulation
Adult animals (2 d after L4 stage) were placed on 6 mM NaCl- or 6 mM VPA-containing plate for 3.5 h, and then they were anesthetized in M9 solution containing 0.1% tricaine, 0.01% tetramisole, and 6 mM NaCl or 6 mM VPA for 20 min. Ovulation and sheath cell contraction were observed as described previously (McCarter et al., 1999; Yin et al., 2004). Brood size was quantified by transferring L4s to fresh drug-containing plates every day for 5 d. The number of F1 progeny on each plate was counted 1 d after eggs hatched.

Growth Assay
Eight to 12 young adult animals (24–26 h post-L4 stage) were placed on drug-containing plate, and then they were allowed to lay eggs for 1 h until 50–60 eggs were laid. The adults were removed, and eggs were left to hatch and develop at 20°C. After 3 and 4 d, the percentage of adult animals on the plates was counted, and representative pictures were taken.

Aldicarb and Levamisole Assays
First-day adult animals (22–24 h after L4 stage) were first placed on plates containing 12 mM NaCl or 12 mM VPA, and, where indicated, 0.25 µg/ml phorbol 12-myristate 13-acetate (PMA) (Sigma Chemical, Poole, Dorset, United Kingdom) or 25 µg/ml methiothepin (Sigma Chemical) for 2 h. Animals were then placed on the same plates, except they also contained either 1 mM aldicarb or 100 µM levamisole. Onset of paralysis was measured as described previously (Nurrish et al., 1999). These assays were performed blind with respect to drug treatments and strains. For each experiment, 25–30 animals were tested, and each experiment was repeated at least four times.

Microscopy
Animals expressing UNC13S::YFP; p.acr-2::SNB-1::CFP (nzIs59) were imaged by mounting on agarose pads, and they were viewed using a Leica TCS SPE confocal microscope with a Leica 63x ACS APO, with a numerical aperture of 1.3. Images were obtained using Leica LAS AF software. Images were blinded with respect to treatment and the eight-bit images were analyzed using ImageJ (http://rsb.info.nih.gov/ij/). UNC-13::YFP and SNB-1::CFP puncta numbers per 10 µm were counted as described previously (McMullan et al., 2006). PHPLC-1::GFP punctual and interpuncta fluorescent values were obtained as described in Marza et al. (2007). Essentially, the images were thresholded, and a puncta was defined as any object within the neuronal process compose of more than five pixels with a value >150 (fluorescent values of an 8-bit image range from 0 to 255). The interpunctal regions were the parts of the neuronal process with a value <150. Eleven wild-type animals exposed to 12 mM NaCl and 11 animals exposed to 12 mM VPA were imaged. Quantification was performed blind to drug treatment.

Egg-laying Assay
Eight young adults (24–26 h after the L4 stage) were placed on a 6 mM NaCl- or 6 mM VPA-containing assay plate, and they were allowed to lay eggs for 2 h. The adults were removed, and the remaining eggs (referred to as "on plate") were counted. The adults were individually dissolved with alkaline bleach solution on unseeded agar plates, and their eggs, which were not dissolved because of their protective eggshells, were counted (referred to as "in worm"). For each condition, five independent experiments were carried out. For PMA experiments, assay plates containing 2 µg/ml phorbol ester (Sigma Chemical) were used.

RNA Interference (RNAi) of Myoinositol-1-phosphate Synthase (MIP Synthase; VF13D12L.1)
RNAi gene knockdown was induced by feeding worms with Escherichia coli (strain HT115) producing double-stranded RNA (dsRNA). Bacteria containing the L4440 vector with inserts of the C. elegans MIP synthase (VF13D12L.1) (Kamath et al., 2003) were obtained from MRC Geneservice. NGM plates containing 25 µg/ml carbenicillin and 1 mM isopropyl β-D-thiogalactoside were allowed to dry overnight before seeding. Bacteria were cultured for 24 h at 37°C in L-Broth containing 50 µg/ml ampicillin, and then they were seeded onto plates. The next day, 4–6 L1 stage larvae were synchronized by treating first-day adults with alkaline bleach on unseeded plates the day before, and they were transferred onto feeding plates and left to develop at 20°C. For defection assay, L4 progenies were transferred to new feeding plates seeded with the bacteria, and defecation assays were performed after 22–24 h. For growth assay, the resultant adults were transferred to new plates seeded with the bacteria, and they were allowed to lay eggs for 1 h until the number of eggs reached 50–60. Adults were removed, and eggs were left to hatch and develop at 20°C. HT115 bacteria transformed with the L4440 vector without an insert were used as an RNAi control.

Quantification of Inositol Phosphates
Three L4s were placed on NGM agar plates seeded with Escherichia coli strain OP50, and after 4 d animals were washed off the plates to start a liquid culture. Two 55-mm plates were used for each 10-ml culture. Harvested animals were grown in S medium (Stiernagle, 2006) supplemented with 200 µg/ml streptomycin, 10 µg/ml nystatin, and 10 µCi/ml myo-[1,2-³H]inositol (American Radiolabeled Chemicals, St. Louis, MO) at 20°C for 4 d. Every day, concentrated E. coli (strain HB101) was supplemented as food. Twenty-four hours before the end of the culture, NaCl, VPA, or LiCl was added to the culture at the final concentration of 12 mM. The measurement of radioactive inositol phosphates was performed as described previously (Azevedo and Saiardi, 2006), with some modification. Briefly, animals were collected and centrifuged, and washed three times with cold distilled water. Worms were first frozen on dry ice, and then an equal volume of 1 M ice-cold HClO₄ containing 3 mM EDTA was added, followed by vortexing for 10 min with an equal volume of glass beads (423–600 µm; Sigma Chemical). The samples were neutralized by an equal volume of 1 M K₂CO₃ containing 3 mM EDTA, and then they were centrifuged to remove precipitates before high-performance liquid chromatography (HPLC) analysis. The composition of inositol phosphates was assessed using a 4.6- x 125-mm Partisphere SAX column (Whatman, Maidstone, United Kingdom). The column was eluted with a gradient generated by mixing buffer A (1 mM EDTA) and buffer B [buffer A plus 1.3 M (NH₄)₂HPO₄, pH 3.8, with H₃PO₄] as follows: 0–5 min, 0% B; 5–10 min, 0–20% B; 10–70 min, 20–100% B; and 70–80 min, 100% B. The fractions (1 ml) were collected and counted using 4 ml of Ultima-Flo AP LCS-mixture (PerkinElmer Life and Analytical Sciences, Boston, MA). The ratio of inositol 1,3,4,5,6 pentakisphosphate and hexakisphosphate (IP₆) to inositol monophosphate (IP₁) was smaller than those observed from mammalian cells (observation by A.S.). This could reflect insufficient labeling time or a genuine different in inositol phosphate levels between C. elegans and mammals.

Statistics
Unpaired Student's t test (two-tailed) or multiple comparisons with Scheffé post hoc tests were used as required for all experiments except for the defecation assay. Because some defecation data were highly variable and did not show a normal distribution, we used nonparametric tests. For comparison of two groups, the Mann–Whitney test was used. For comparison of more than three groups, the Kruskal–Wallis test was first performed, and then when it was significantly different, the Mann–Whitney test was performed to compare each pair of groups. Data are shown as mean ± SEM.

	RESULTS

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES

 
VPA Disrupts Defecation, a Known IP₃-mediated Behavior
To investigate the action of VPA on inositol phosphate signaling in C. elegans, we compared the effects of VPA with Li⁺, a known inhibitor of enzymes required for inositol phosphate recycling. We began by testing the effects of VPA and Li⁺ on defecation, which is known to be regulated by IP₃. The C. elegans defecation cycle is characterized by the contraction of three distinct sets of muscles every 50 s (Thomas, 1990). It begins with contraction of the posterior body wall muscles (pBoc), which is then followed by the relaxation of these muscles and the contraction of the anterior body wall muscles; finally, the enteric muscles contract, resulting in expulsion of the gut contents. Either mutations in IP₃R (itr-1) or overexpression of the IP₃ binding domain of the receptor from a ubiquitous heat-shock promoter causes a large increase in the time between defecation cycles (Dal Santo et al., 1999; Walker et al., 2002). VPA (valproic acid sodium salt) increased the mean defecation cycle length (defined as the time between pBoc steps) in a dose-dependent manner; LiCl also increased mean defecation cycle length, although to a lesser extent, whereas NaCl had no effect (Figure 1A). In addition, VPA increased the variability of defecation cycle length, as shown by defecation cycles for representative animals in Figure 1B. We quantified this by determining the coefficient of variance of defecation cycle length (Figure 1C). LiCl also increased the variability of defecation cycle length, to a lesser extent (Figure 1, B and C).

View larger version (25K): [in this window] [in a new window]   Figure 1. VPA affects defecation behaviors. (A) Exposure to VPA and Li+ increased C. elegans defecation cycle length in a dose-dependent manner. (B) Examples of defecation cycle length variability of individual animals exposed to 12 mM NaCl, 12 mM VPA, 12 mM LiCl, or 40 mM LiCl. VPA, but not Li+, greatly increased the variability of cycle length from one cycle to the next. (C) The coefficient of variance (SD divided by the mean) of defecation cycle length of animals exposed to the indicated drug concentrations. VPA causes defecation cycle length to become irregular. (D) RNAi knockdown of MIP synthase caused an increase in average defecation cycle length. (E) Examples of defecation cycle length of four individual animals from D are shown. (F) RNAi knockdown of MIP synthase increased the variability of defecation cycle length. However, the effect was not statistically significant (p > 0.06). Except B and E each data point represents mean ± SEM from eight to 22 animals. *p < 0.05 and **p < 0.01; Mann–Whitney test, compared with NaCl controls at the same concentration.

VPA Inhibits Ovulation
In C. elegans, ovulation occurs when an oocyte triggers contractions in the surrounding sheath cells, eventually resulting in the oocyte entering into the spermatheca where it is then is fertilized. IP₃ receptor mutations decrease both contractions of the sheath cells surrounding the oocyte and ovulation itself (Clandinin et al., 1998; Miller et al., 2003; Yin et al., 2004). Mutations that decrease IP₃ synthesis also decrease both ovulation and sheath cell contractions; these include RNAi knockdowns of the phosphatidylinositol-4-phosphate 5' kinase (ppk-1) and the PLC (PLC-3) (Yin et al., 2004; Xu et al., 2007). Defects in ovulation lead to a decreased brood size and addition of VPA strongly decreased brood size (Figure 2A). Exposure to VPA for 4 h also decreased ovulation (1.7 ± 0.2 per hour in the presence of NaCl compared with 0.7 ± 0.2 per hour in the presence of VPA) (Figure 2B). VPA also decreased the basal sheath cell contraction rate (9.0 ± 0.6 contractions per minute on NaCl compared with 5.3 ± 0.6 contractions per minute in the presence of VPA) (Figure 2C). These results are consistent with a model in which VPA decreases IP₃ signaling.

View larger version (27K): [in this window] [in a new window]   Figure 2. VPA reduces brood size, ovulation, and gonadal-sheath cell contractions. (A) VPA reduced the brood size of wild-type animals. (B) VPA (6 mM) reduced ovulation (passage of an oocyte into the spermatheca). (C) VPA (6 mM) reduced the basal sheath cell contraction rate in wild-type and control unc-24(e138) animals, but not in animals possessing an itr-1 gain-of-function mutation [itr-1(sy327) unc-24(e138)]. **p < 0.01 and ***p < 0.001; Student's test, compared with NaCl controls at the same concentration. Each data point represents mean ± SEM from 10 animals.

View larger version (34K): [in this window] [in a new window]   Figure 3. An IP3 receptor gain-of-function mutation suppresses the effects of VPA. (A) Exposure to 6 mM VPA increased the mean defecation cycle length of itr-1(sy327) unc-24(e138) animals less than wild-type or control unc-24(e138) animals. (B) Examples of defecation cycle length variability of individual animals exposed to 6 mM NaCl or 6 mM VPA. (C) Coefficient of variance of animals exposed to 6 mM NaCl or 6 mM VPA. VPA increased the variability of defecation cycle length less in itr-1(sy327) unc-24(e138) animals compared with wild-type or control unc-24(e138) animals. In A and C, each data point represents mean ± SEM from 10 to 17 animals. *p < 0.05 and ***p < 0.001; Mann–Whitney test.

View larger version (33K): [in this window] [in a new window]   Figure 4. VPA reduces ACh release. Levels of ACh release were measured by determining rates of paralysis in the presence of the acetylcholinesterase inhibitor aldicarb either in the absence (A, C, E, and G) or presence of the phorbol ester PMA (B, D, F, and H). In all cases, there was no significant statistical difference between NaCl and VPA treatments when tested in the presence of PMA. (A and B) Exposure to 12 mM VPA caused animals to become resistant to aldicarb compared with untreated animals and animals exposed to 12 mM NaCl (A). Addition of the DAG analogue PMA caused hypersensitivity to aldicarb; this sensitivity was not altered by addition of 12 mM VPA or 12 mM NaCl (B). In A, at 80 min the percentage of animals paralyzed in the absence of drug was 81.6 ± 8.1%, on NaCl 84.2 ± 4.7%, on VPA 36.1 ± 6.0%; errors are SEM, using an unpaired Student's t test the difference between no drug and VPA, p = 0.0093, and between NaCl and VPA, p = 0.0002. There was no significant statistical difference for any of the drug treatments when combined with PMA. (C–F) unc-13(s69) animals were rescued with a transgene that expresses either a non–DAG-binding form of the UNC-13 neuromodulator [unc-13(s69);UNC-13(H173K)::GFP, referred to in the text as UNC-13(H173K) animals] (C and D) or by a transgene expressing a wild-type form of UNC-13 [unc-13(s69);UNC-13::GFP referred to in the text as UNC-13(+) animals] (E and F). UNC-13(H173K) animals are resistant to aldicarb (at 80 min, paralysis is 17.8 ± 6.0% SEM), and this is not further increased by the addition of 12 mM VPA (at 80 min, paralysis 18.5 ± 6.9% SEM, difference from NaCl, p = 0.9336 using unpaired Student's t test) (C). unc-13(s69);UNC-13 (H173K) animals are partially resistant to the effects of PMA (D). VPA (12 mM) decreases the aldicarb sensitivity of UNC-13(+) animals the same as wild-type animals (at 80 min, on NaCl paralysis 86.3 ± 1.4% SEM compared with VPA paralysis 35.4 ± 7.7% SEM, p = 0.0003 using unpaired Student's t test) (E). UNC-13(+) animals are hypersensitive to aldicarb in the presence of PMA, and this sensitivity is not affected by exposure to 12 mM VPA (F). (G and H) A mutation in the EGL-8 PLBβ [egl-8(md1971)] results in resistance to aldicarb (at 80 min on NaCl paralysis is 23.5 ± 10.6% SEM), and this sensitivity is not further increased by the addition of 12 mM VPA (at 80 min, paralysis 21.8% ± 9.4, difference from NaCl, p = 0.9 using unpaired Student's t test) (G). Addition of the DAG analogue PMA caused hypersensitivity to aldicarb, this is not suppressed in egl-8(md1971) mutant animals (H).

View larger version (30K): [in this window] [in a new window]   Figure 5. VPA decreases UNC-13 accumulation but not synapse numbers. (A) The serotonin antagonist methiothepin causes animals to become hypersensitive to aldicarb-induced paralysis. This effect is reversed by exposure to VPA (on methiothepin and NaCl paralysis at 60 min is 99.2% ± 0.9 SEM, on methiothepin and VPA paralysis is 74.1 ± 5.9% SEM, p = 0.003, using unpaired Student's t test). (B and C) The serotonin antagonist methiothepin causes UNC-13::YFP to become punctate in a DAG-dependent manner (Nurrish et al., 1999), and this is suppressed by exposure to 12 mM VPA. In B, each data point represents mean ± SEM from 14 animals (p < 0.05, Student's t test). In C, digital images were converted from grayscale into a 32-color lookup table (ImageJ) to visualize pixel intensities. Arrows point to puncta counted in B. Bar, 10 µm. (D) The integral membrane protein SNB-1::CFP is enriched in synaptic vesicles; thus, it acts as a marker for synaptic vesicle release sites. VPA did not alter the density of SNB-1::CFP puncta.

View larger version (33K): [in this window] [in a new window]   Figure 6. VPA inhibits egg-laying behavior via DAG signaling. The number of eggs laid on the plate over 2 h, and the number of eggs remaining in the animals at the end of the 2 h were determined. (A) VPA inhibits egg laying. Exposure to 6 mM VPA reduced the number of eggs laid on the plate compared with 6 mM NaCl (*p < 0.05, Student's t test). (B and C) unc-13(s69) animals were rescued with a transgene that expresses either a non–DAG-binding form of the UNC-13 neuromodulator [unc-13(s69);UNC-13(H173K)::GFP, referred to in the text as UNC-13(H173K) animals] (B) or by a transgene expressing a wild-type form of UNC-13 [unc-13(s69);UNC-13::GFP referred to in the text as UNC-13(+) animals] (C). Egg laying by UNC-13(H173K) animals is unaffected by addition of 6 mM VPA (B), whereas egg laying by UNC-13(+) animals is inhibited by 6 mM VPA (C). (D) A mutation in the EGL-8 PLBβ [egl-8(md1971)] causes an egg-laying defect, and this is not altered by addition of 6 mM VPA. (E–G) The addition of DAG analogue PMA reverses the inhibition of egg laying caused both by exposure to 6 mM VPA in wild-type (E) animals and by mutation of the PLCβ EGL-8 (G), but not the inhibition caused by disruption of a DAG effector, UNC-13(H173K) animals (F). Each datum represents mean ± SEM from five independent experiments. *p < 0.05, **p < 0.01, and ***p < 0.001, Scheffé's multiple comparison test.

View larger version (31K): [in this window] [in a new window]   Figure 7. VPA inhibits ACh release and egg laying in a pkc-1 mutant. (A) Levels of ACh release were measured by determining rates of paralysis in the presence of the acetylcholinesterase inhibitor aldicarb. pkc-1(nj3) mutant animals are resistant to aldicarb induced paralysis compared with wild type in the absence of salt (data not shown) and in the presence of 12 mM NaCl. Exposure to 12 mM VPA caused both wild-type and pkc-1(nj3) mutant animals to become resistant to aldicarb (For pkc-1(nj3), paralysis at 80 min on NaCl is 68.9 ± 5.0% SEM, on VPA 21.9 ± 8.7% SEM, p = 0.0008, using unpaired Student's t test). (B) Exposure to 6 mM VPA caused pkc-1(nj3) mutant animals to lay fewer eggs on the plate than animals exposed to 6 mM NaCl. Exposure to 6 mM VPA did not affect the total number of eggs produced (*p < 0.05, Student's t test).

View larger version (32K): [in this window] [in a new window]   Figure 8. VPA decreases IP1 and increases PIP2. (A) Illustration of a simplified inositol phosphate cycle. PI(4,5)P2 is hydrolyzed by PLC enzymes to generate IP3 and DAG. IP3 is degraded by sequential dephosphorylations to myoinositol. The enzymes IPPase and IMPase are sensitive to inhibition by lithium. MIP synthase catalyzes the conversion of glucose-6-phosphate (glucose-6-P) to IP1 in inositol de novo synthesis. DAG is converted to phosphatidic acid (PA). PA is converted to cytidine diphosphodiacylglycerol (CDP-DAG). PI is synthesized from CDP-DAG and myoinositol by PI synthase. In C. elegans Ins(1,4,5)P3 induces intracellular Ca2+ release, which is required for correct rates of defecation and growth. DAG acts via UNC-13 and PKC-1 to increase ACh release at neuromuscular junctions and stimulate egg laying. (B) Typical HPLC elution profiles of soluble inositol polyphosphates from C. elegans radiolabeled with [3H]inositol for 4 d. Shown are elution profiles for animals exposed to 12 mM NaCl or VPA. IP1, IP2, IP3, and IP6. (C) VPA (12 mM) reduces levels of IP1. Shown are the amounts of IP1, IP2, and IP3 in animals treated with 12 mM VPA compared with animals treated with 12 mM NaCl (set to 100%). Levels of IP2, IP3, and IP6 were not significantly changed. (D) The PI(4,5)P2 reporter PHPLC-1::GFP expressed from the rab-3 promoter has a punctate pattern in the sublateral dorsal nerve cord of wild-type animals exposed to either 12 mM NaCl (top) or 12 mM VPA (bottom). (E) Quantification of PHPLC-1::GFP fluorescence in the sublateral dorsal nerve cord. Average pixel intensity within puncta was unchanged by exposure to 12 mM VPA, although average pixel intensity between puncta was increased (***p < 0.001 and *p < 0.01, Student's t test; error bar, SEM).

VPA Does Not Decrease PIP₂ Levels
VPA causes behavioral defects consistent with decreases in both DAG and IP₃ levels. The simplest explanation for these results is that VPA inhibits either one or more PLCs or an enzyme required for PIP₂ production. In the former case, we would predict PIP₂ levels to be unchanged or increased, whereas in the latter case we would predict a decrease in PIP₂ levels. We first attempted to quantify PIP₂ levels as we had for inositol polyphosphates, but our results were too variable to give meaningful results. We therefore visualized PIP₂ levels using PHPLC-1::GFP, which binds selectively to PtdIns(4,5)P₂ (Varnai and Balla, 1998; Patton et al., 2005). We obtained animals expressing PHPLC-1::GFP from the pan-neuronal rab-3 promoter that has been used in C. elegans to demonstrate changes in levels of PIP₂ in animals defective for polyunsaturated fatty acid production (Marza et al., 2007). As reported previously (Marza et al., 2007), we observed a punctate distribution of PHPLC-1::GFP in the sublateral neuronal processes of untreated animals (Figure 8D). We were unable to distinguish individual puncta in the dorsal and ventral cords because they contained too many neurons; therefore, we measured PHPLC-1::GFP fluorescence at sublateral neurons (Marza et al., 2007). On addition of 12 mM VPA, we observed a slight increase in the PHPLC-1::GFP fluorescence at puncta, but this was not statistically significant (189 ± 20 12 mM NaCl vs. 199 ± 20 12 mM VPA; p = 0.2) (Figure 8, D and E). VPA (12 mM) did cause a significant increase in the amount of PHPLC-1::GFP fluorescence present between puncta (103 ± 6 12 mM NaCl vs. 123 ± 15 12 mM VPA; p = 0.01) (Figure 8, D and E). Ideally, we would want to observe changes in PHPLC-1::GFP fluorescence in mutants predicted to have changes in PIP₂ levels. A mutation in the single C. elegans ppk-1 would be expected to eliminate PIP₂; however, ppk-1 mutations are lethal (Weinkove et al., 2008). Thus, the results with PHPLC-1::GFP must be treated with care, but they suggest that VPA does not decrease levels of PIP₂ within neurons, and they may act to slightly increase PIP₂ levels.

	DISCUSSION

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES

 
In this report, we show that VPA affects IP₃-regulated behaviors, defecation, and ovulation, confirming that VPA affects inositol signaling. We also show that VPA affects DAG-regulated behaviors locomotion and egg laying. VPA seems to affect DAG signaling via UNC-13 more than DAG signaling by PKC-1.

To test for a role of VPA in inositol phosphate signaling in C. elegans, we compared the effects of VPA with defects at three steps in this signaling pathway: a defect in production of inositol (RNAi knockdown of MIP synthase), inhibition of the inositol phosphate recycling enzymes IMPase and IPPase (addition of Li⁺), and a defect in the IP₃ receptor (Dal Santo et al., 1999; Walker et al., 2002). Exposure to VPA causes the same phenotype as all three of these methods of disrupting inositol phosphate signaling—increased defecation cycle length. In addition, VPA decreased ovulation, which is another IP₃-regulated behavior (Clandinin et al., 1998). An important question is whether VPA acts on IP₃ signaling directly or on a parallel signaling pathway. Gain-of-function mutations in the IP₃ Receptor have been shown to suppress defecation and ovulation defects caused by lowered amounts of IP₃ production (Yin et al., 2004; Espelt et al., 2005). The same IP₃ receptor gain-of-function mutations also suppress defects in ovulation and defecation caused by VPA. This suggests that VPA reduces levels of IP₃ signaling at a point upstream of the IP₃ receptor, probably by inhibiting IP₃ production. VPA has also been shown to decrease inositol phosphate signaling, in particular IP₃ signaling, in mammalian neuronal cell cultures and in Dictyostelium (Williams et al., 2002). Extraction of labeled inositol phosphates from whole C. elegans revealed that VPA does decrease IP₁, although levels of IP₃ are not significantly affected. This conflicts with behavioral assays that predict a defect in IP₃ levels. Our chromatographic analyses do not allow the separation of the different isomeric inositol polyphosphates species; the IP₃ peak is therefore a mixture of the several IP₃ isomers, and we cannot conclude that levels of I(1,4,5)P₃ are unchanged. In addition, the target of VPA may only exist in a subset of cells, and so changes in IP₃ levels in a small number of cells may not be detected when assaying whole animals. It is also possible that IP₁ itself may have an important role in the control of both defecation and ovulation and that VPA alters both by decreasing IP₁ levels, although IP₃ receptor gain-of-function mutations suppressed VPA-mediated defects in both defecation and ovulation, suggesting that VPA does alter both behaviors by changing IP₃ levels. Improvements in the detection of labeled inositol phosphates would allow us to tell which is the correct explanation. Nontheless, PLC-generated I(1,4,5)P₃ is rapidly metabolized and recycled through the "inositol cycle" (Figure 7A) to form inositol. IP₁ is a key element of this important metabolic cycle, and its significant decrease after exposure to VPA suggests that VPA alters both inositol phosphate recycling and signaling.

RNAi of MIP synthase should decrease the formation of inositol from glucose, an effect that should be relieved by addition of inositol. Indeed, addition of 200 mM myoinositol rescues the thermotaxis defects in ttx-7 (IMPase) mutants, which cannot produce inositol (Tanizawa et al., 2006). However, 200 mM myoinositol caused defecation defects to wild-type animals (data not shown), and we were unable to test whether myoinositol could rescue the defects caused by exposure to VPA.

In addition to the inhibition of IP₃-regulated behaviors by VPA, we show that VPA inhibits DAG-regulated behaviors—ACh release and egg laying. In the first case, exposure to VPA decreases levels of ACh released by motor neurons onto body wall muscles, as shown by a decreased rate of paralysis by aldicarb. In the second case, VPA decreases the rate of egg laying. In both cases, VPA mimics mutations in egl-8 PLCβ, pkc-1, and animals in which a severe unc-13 mutant is rescued by a transgene expressing a non–DAG-binding UNC-13 mutant (UNC-13(H173K) animals). Addition of VPA does not further decrease locomotion and egg laying in either egl-8 PLCβ mutants or UNC-13(H173K) animals. However, in pkc-1 mutants, VPA is still able to reduce both ACh release (as shown by increased resistance to aldicarb) and egg laying. We cannot definitely rule out an effect of VPA on signaling via PKC-1, but it does seem that DAG signaling via UNC-13 is more sensitive to VPA than DAG signaling via PKC-1. It is also possible that VPA affects DAG signaling via other DAG effectors, for example, other PKCs.

What is the relevant target(s) of VPA? In mammals, VPA has been shown to inhibit the activity of a prolyl oligopeptidase (Cheng et al., 2005); however, there is no obvious orthologue in C. elegans. Thus, the relevant VPA target(s) remains to be identified in C. elegans. Our data show that VPA acts to inhibit behaviors regulated by both inositol phosphates and DAG. Because both DAG and IP₃ are derived from PIP₂, it is possible that VPA causes a defect at or before PIP₂ production (Figure 7A). Use of a PIP₂ reporter suggests than neuronal levels of PIP₂ are slightly increased upon exposure to VPA. The simplest explanation for these data are that VPA inhibits a step after the production of PIP₂ but before the production of DAG and IP₃, probably all or a subset of PLCs, or a common regulator of PLCs. Alternatively, VPA could inhibit the removal of PIP₂ by a lipid phosphatase such as synaptojanin (UNC-26) (Harris et al., 2000), causing an increase in PIP₂ levels. We observed that PIP₂ levels were higher between the normal sites of PIP₂ accumulation, and this could cause a delocalization of PIP₂ binding effectors, leading to a decrease in the ability to generate DAG and inositol phosphates. VPA also caused an increase in muscle response to the nicotinic agonist levamisole, and this occurred independently of signaling via EGL-8 and UNC-13. Currently, we do not know whether VPA alters muscle responsiveness via changes in DAG and/or IP₃ signaling, or via a third mechanism.

VPA inhibited both defecation and ACh release in adults within 2 h, suggesting VPA acts to disrupt neuronal function and not development. The concentration of VPA (6–12 mM) used is 10-fold higher than the recommended therapeutic blood serum concentrations (0.3–0.6 mM); however, we can only measure the external VPA concentration. Because of the relatively impermeable C. elegans cuticle it is likely the actual internal VPA concentration will be much closer to those used to treat bipolar disorder in humans. Thus, signaling pathways disrupted in C. elegans may be the same pathways disrupted in humans treated with VPA. Our results raise the possibility that VPA relieves the symptoms of bipolar disorder due to its action on DAG signaling pathways that regulate neurotransmitter release. However, both DAG and inositol phosphates have been implicated in the regulation of neuronal function, and we favor a model in which VPA helps control the symptoms of bipolar disorder by regulating neuronal function through its effects on both inositol phosphate and DAG signaling pathways.

	ACKNOWLEDGMENTS

 
We thank other members of the Nurrish laboratory for advice and discussion, and Anne Mudge, Martin Raff, and Giovanni Lesa for comments on the manuscript. Strains were obtained from the C. elegans Genetics Center, which is supported by the National Institute of Health National Center for Research Resources. S.M.T. was supported by the Ernst Schering Foundation.

	Footnotes

 
This article was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E07-09-0982) on February 20, 2008.

* Present address: Department of Biochemistry and Molecular Biology, Faculty of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.

Address correspondence to: Stephen J. Nurrish (s.nurrish{at}ucl.ac.uk)

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