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Figure 5. -Syn-EGFP accumulations do not disrupt organization of the ER, vacuole, or endosomes. (A) Presence of -syn-EGFP accumulations does not disrupt localization of the ER marker Sec63p. Sec63p was C-terminally tagged with monomeric RFP. Expression of EGFP or -syn-EGFP shows similar localization of the ER marker. (B) Presence of -syn-EGFP accumulations does not disrupt localization of the vacuole membrane marker Vma4p, which was C-terminally tagged with monomeric RFP. (C) Presence of -syn-EGFP accumulations does not disrupt localization of the endosomal marker, Vps17p, which was C-terminally tagged with dimeric RFP. Scale bar, 2 µm.
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