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Vol. 20, Issue 18, 3986-3995, September 15, 2009
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*Center for Priority Areas, Tokyo Metropolitan University, Hachioji, Tokyo 192-0397, Japan; Department of Chemistry, Graduate School of Sciences and Engineering, Tokyo Metropolitan University, Hachioji, Tokyo 192-0397, Japan; Innovative Drug Research Laboratories, Kyowa Hakko Kirin Co., Ltd., Takasaki, Gumma, 370-1295, Japan; and Department of Molecular Genetics, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, Shizuoka 411-8540, Japan
Submitted January 22, 2009;
Revised July 10, 2009;
Accepted July 13, 2009
Monitoring Editor: Wendy Bickmore
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ABSTRACT |
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 TOP ABSTRACT INTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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INTRODUCTION |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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-satellite DNA in human cells, the DNA sequences themselves are thought to be neither necessary nor sufficient for determination of centromere position (Cheeseman and Desai, 2008
). Studies of neocentromeres have shown that functional centromeres can be ectopically constructed and stably maintained at loci that do not possess -satellite sequences (Marshall et al., 2008). This indicates that the centromeric region is specified by epigenetic mechanisms rather than by cis-acting elements. One candidate for an epigenetic marker that specifies centromeres is CENP-A (Black and Bassett, 2008). CENP-A is localized at functional centromeres, including neocentromeres and is required for the centromeric localization of other centromere proteins (Warburton et al., 1997; Howman et al., 2000; Oegema et al., 2001; Goshima et al., 2003; Regnier et al., 2005; Liu et al., 2006). Ectopic mislocalization of human CENP-A, or its Drosophila counterpart CID, induces the colocalization of other centromere proteins to the noncentromeric loci (Van Hooser et al., 2001; Heun et al., 2006). Overall, the available information indicates that CENP-A is the key player for targeting centromere proteins to the correct sites by acting as an epigenetic marker. Thus, to understand how the centromere is specified in the correct chromosomal region, it is important to elucidate the mechanisms that target CENP-A to centromeric chromatin.
Recent studies identified various proteins involved in the centromeric localization of CENP-A. RbAp46/48, hMis18/β, and M18BP1/KNL2 are required for centromeric localization of CENP-A in vertebrates and nematodes (Hayashi et al., 2004; Fujita et al., 2007; Maddox et al., 2007). HJURP binds to CENP-A and is required for centromeric localization of CENP-A in vertebrates (Foltz et al., 2009; Dunleavy et al., 2009). CENP-A mislocalization in hMis18 depleted cells is rescued by trichostatin A, suggesting that the hMis18 complex might acetylate histones or centromere localized proteins to enhance CENP-A deposition.
The constitutive centromere associated network (CCAN) proteins have been found to localize to centromeres throughout the cell cycle and have important roles in the construction of functional kinetochores (Cheeseman and Desai, 2008). We previously identified the CENP-H-I complex as a member of the CCAN in chicken and human cells. The CENP-H-I complex can be subdivided into several groups. The CENP-H–containing subcomplex, including CENP-H, -I, -K, and -M, is required for the efficient targeting of newly synthesized CENP-A into centromeres (Okada et al., 2006), but it does not include typical chromatin remodeling factors. This suggests that the CENP-H–containing complex might act in concert with other proteins that function to remodel chromatin structures.
Recent proteomic analyzes revealed that several chromatin remodeling factors and histone chaperones are associated with CENP-A–containing chromatin (Obuse et al., 2004; Foltz et al., 2006). On the basis of published data, we hypothesized that the chromatin remodeling complex named facilitates chromatin transcription (FACT) might be involved in CENP-A incorporation into centromeric chromatin. FACT was first reported as a factor that enhances transcription elongation from chromatin templates in vitro (Orphanides et al., 1998), and in budding yeast, FACT was shown to alter chromatin structure without using ATP hydrolysis (Ruone et al., 2003; Rhoades et al., 2004). In fission yeast, FACT contributes to centromeric heterochromatin formation (Lejeune et al., 2007). In addition, Drosophila FACT enhances the deposition of histone H3.3 on the nucleosomes adjacent to FACT binding sites (Nakayama et al., 2007), suggesting that FACT alters chromatin structure to make the chromatin competent for replacement of canonical histone H3 by histone H3 variants. These findings raise the possibility that the centromere localized FACT plays an important role in deposition of the centromere-specific histone H3 variant CENP-A into the centromeric chromatin.
To elucidate the possible role of the CENP-H–containing complex in deposition of newly synthesized CENP-A into the centromeric chromatin, we investigated the functional interaction of the CENP-H–containing complex with FACT. Here, we report that FACT localizes to centromeres in the CENP-H–containing complex dependent manner and that FACT is required for the efficient incorporation of newly synthesized CENP-A into centromeres. In addition, the ATP-dependent chromatin remodeling factor CHD1 is localized to centromeres through a physical interaction with FACT and is required for the maintenance of the amount of CENP-A at centromeres. Therefore, the CENP-H–containing complex seems to facilitate CENP-A incorporation into centromeric chromatin in cooperation with FACT and CHD1.
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MATERIALS AND METHODS |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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). All DT40 cells were cultured at 38.5°C in Dulbecco's modified medium supplemented with 10% fetal calf serum, 1% chicken serum, penicillin, and streptomycin. To repress the expression of the tet-responsive transgenes, tetracycline (Sigma-Aldrich, St. Louis, MO) was added to the culture medium to a final concentration of 2 µg/ml. Fluorescence-activated cell sorting (FACS) analysis was performed as described previously (Fukagawa et al., 2001). For the CENP-A-GFP incorporation assay, cells were resuspended in Nucleofector T reagent containing 2 µg of cytomegalovirus (CMV)-chicken CENP-A-green fluorescent protein (GFP) plasmid and 1 µg of CMV-monomeric red fluorescent protein (mRFP) plasmid and electroporation was performed using a Nucleofector instrument (Amaxa Biosystems, Gaithersburg, MD). Cells were cultured for 16 h and fixed with 3% paraformaldehyde. In each experiment, only the cells expressing mRFP signals were counted for scoring the efficiency of CENP-A incorporation.
Human HeLa S3 cells were cultured on a seesaw shaker placed in a CO2 incubator for suspension culture. Control small interfering RNAs (siRNAs) or siRNAs against CHD1 (Invitrogen, Carlsbad, CA) were transfected using Lipofectamine RNAi MAX (Invitrogen). The predesigned siRNAs against CHD1 and control siRNAs can be ordered as Stealth Select RNAi reagent HSS101841 and Stealth RNAi Negative Control LO GC (12935-200), respectively.
Immunocytochemistry
Cells were fixed with 3% paraformaldehyde and incubated with antibodies in 0.5% bovine serum albumin (BSA) for 1 h. DNA was counterstained with 4,6-diamidino-2-phenylindole (DAPI). For staining with anti-SSRP1, anti-SPT16, and anti-CHD1 antibodies, cells were treated with nocodazole (1 µg/ml) for 2 h before fixation to accumulate mitotic cells. Cells were then extracted with 0.1% Triton X-100 in phosphate-buffered saline (PBS) for 2 min followed by 15-min fixation with 3% paraformaldehyde. Antibody incubations were performed for 1 h by using CanGetSignal Immunostaining Solution A (Toyobo Engineering, Osaka, Japan). CHD1 was detected using N-16 polyclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA) and human CENP-A by using D115–3 monoclonal antibody (MBL International, Woburn, MA). All images were collected with a cooled charge-coupled device camera (CoolSNAP HQ, Photometrics Image Point; Photometrics, Tucson, AZ) mounted on an IX71 inverted microscope (Olympus, Tokyo, Japan) with a 60x objective lens (PlanApo 60x, numerical aperture 1.40) together with a filter wheel. Images were analyzed with an IPLab software (Signal Analytics, Vienna, VA). All images for a given condition were acquired, processed, and scaled identically.
Glutathione Transferase (GST)-Pull Down Assay
Bacterially expressed GST-tagged chicken SSRP1 polypeptides were conjugated with glutathione-Sepharose beads (GE Healthcare, Little Chalfont, Buckinghamshire, United Kingdom) and incubated with in vitro translated proteins for 2 h at 4°C in buffer A (20 mM HEPES-KOH, pH 7.4, 150 mM KCl, 10 mM MgCl2, 10% glycerol, 0.1% NP-40, 1% BSA, and 50 µg/ml ethidium bromide) and then washed with buffer B (20 mM HEPES-KOH, pH 7.4, 150 mM KCl, 10 mM MgCl2, 10% glycerol, and 0.1% NP40). Benzonase nuclease (100 U) (Novagen, Madison, WI) was added separately to the in vitro-translated proteins and purified GST-tagged SSRP1 and incubated for 1 h at 25°C before the binding reaction when described.
Microarray
Total RNA was prepared from SSRP1 conditional mutant cells cultured in the absence or presence of tet for 42 h and subjected to biotin labeling according to the manufacturer's protocols. Gene expression profiles were analyzed using GeneChip Chicken Genome Array (Affymetrix, Santa Clara, CA).
DNA Data Bank of Japan/European Molecular Biology Laboratory/GenBank Accession Numbers
The nucleotide sequences of cDNA for chicken SSRP1 and CHD1 are listed under accession AB465209 and AB465210, respectively.
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RESULTS |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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; Foltz et al., 2006). We also found that both SSRP1 and SPT16 coimmunoprecipitated with CENP-A-GFP from chicken DT40 cells by a similar method to others (Supplemental Figure S1). However, the precise cellular localization of SSRP1 and SPT16 had not yet been determined. To investigate whether FACT localizes to centromeres, we analyzed the localization of endogenous SSRP1 and SPT16 by using specific antibodies. The chicken DT40 cell line that stably expresses CENP-H-RFP was used to enable visualization of centromere positions. As reported previously (Kelley et al., 1999), in cells fixed with paraformaldehyde, we obtained strong signals for both SSRP1 and SPT16 throughout the nuclei (Figure 1A). In contrast, when cells were treated with 0.1% Triton X-100 to eliminate soluble proteins before fixation, we found that both SSRP1 and SPT16 colocalized with CENP-H-RFP (Figure 1B). In interphase nuclei, immunostaining for SSRP1 and SPT16 proteins showed strong signals at centromeres, but some signals were also present at noncentromeric positions. At mitosis, SSRP1 and SPT16 were mainly localized at the centromeres. These results demonstrate that FACT associates with centromeres throughout the cell cycle.
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) or DNA polymerase (Zhou and Wang, 2004), we postulated that one or more members of the CCAN might be involved in the centromeric localization of FACT. To test this possibility, we immunostained the conditional mutant cell lines for CENP-K, CENP-M, and CENP-C with anti-SSRP1 or anti-SPT16 antibodies. In these conditional mutant cell lines, expression of the corresponding CENPs is blocked by the addition of tet to the medium. CENP-A-RFP expression was used to visualize centromere loci in these cells. We found that the centromeric localization of both SSRP1 and SPT16 was abolished at 48 h after the addition of tet in CENP-K mutant cells and at 72 h in CENP-M mutant cells. In contrast, in CENP-C mutant cells, both SSRP1 and SPT16 were present in centromeres after the addition of tet (Figure 2 and Supplemental Figure S2). We reported previously that the centromeric localization of the CENP-H–containing complex was not dependent on CENP-C (Kwon et al., 2007; Hori et al., 2008). These results suggest that the CENP-H–containing complex but not CENP-C is required for centromeric localization of FACT.
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). As is shown in Figure 2, centromeric localization of FACT is dependent on the CENP-H–containing complex but not CENP-C. Therefore, we hypothesized that centromere localized FACT plays an important role in centromeric targeting of nascent CENP-A. To test whether FACT is required for CENP-A incorporation into centromeres, we generated SSRP1 conditional mutants in which the viability of homozygous mutant cells was maintained by expression of SSRP1 cDNA under the control of a tet-repressible promoter (Supplemental Figure S3). We found that the SSRP1 protein decreased to an undetectable level by 36 h after the addition of tet in the SSRP1 conditional mutant cells (Figure 3A).
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Because SSRP1 and SPT16 form a heterodimer, we tested whether SSRP1 was required for the centromeric localization of SPT16. CENP-A-RFP was used to visualize the centromere loci in the SSRP1 conditional mutant cells. We found that both SSRP1 and SPT16 had disappeared from the centromeres at 45 h after the addition of tet (Figure 3B).
SSRP1 conditional mutant cells stopped proliferating 
48 h after the addition of tet (Figure 3C). We examined the cell cycle profile by measuring both cellular DNA content and DNA synthesis by using FACS after pulse labeling with 5-bromo-2'-deoxyuridine (BrdU). SSRP1-deficient cells started dying at 48 h after the addition of tet but did not show arrest at any particular cell cycle stage (Figure 3D). FACT is known to be involved in diverse cellular functions (Reinberg and Sims, 2006), such as transcription regulation (Shimojima et al., 2003; Nakayama et al., 2007), replication (Zhou and Wang, 2004), and repair (Heo et al., 2008). Thus, SSRP1 seems to be required for cell viability, and SSRP1-deficient cells may have died because of defects in these vital functions.
Newly synthesized CENP-A Is Not Efficiently Incorporated into Centromeres in SSRP1-deficient Cells
To investigate whether FACT is required for centromeric targeting of newly synthesized CENP-A, a CENP-A-GFP–expressing plasmid was transiently transfected into SSRP1 conditional mutant cells that had been cultured for 30 h in the presence of tet; the localization of CENP-A-GFP was analyzed at 16 h after transfection (Figure 4A). In the absence of tet, 82% of the cells exhibited centromeric localization of transiently expressed CENP-A-GFP. In contrast, the efficiency of centromeric targeting of nascent CENP-A-GFP was significantly reduced (14%) in SSRP1-depleted cells (Figure 4C). To determine whether this effect was specific to CENP-A, the efficiency of incorporation of transiently expressed histone H3-GFP was examined. In contrast to CENP-A incorporation, histone H3-GFP was efficiently assembled into the chromatin both in the presence or absence of tet (Figure 4C). These data show that FACT is required for efficient incorporation of newly synthesized CENP-A into centromeres.
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; Jansen et al., 2007), multiple times of cell cycle progression in the SSRP1-depleted condition should be required to detect the reduction of pre-existing CENP-A signals. However, as is shown in Figure 3, SSRP1 conditional mutant cells immediately stopped proliferating when SSRP1 was depleted, we could not detect decrease in the signal intensities of pre-existing CENP-A at centromeres (Figure 3B). To further confirm the requirement of SSRP1 for CENP-A incorporation into centromeres, we analyzed the domain of SSRP1 that is required for CENP-A incorporation. The GST-fused polypeptides of SSRP1 containing N-terminal region (1-193 aa), pleckstrin homology (PH) domain (194-544 aa), high-mobility group (HMG) domain (545-613 aa), and C-terminal region (614-706 aa) (Supplemental Figure S5A) were bacterially expressed and incubated with in vitro translated chicken CENP-A. We found that the C-terminal region of SSRP1 binds to CENP-A in vitro (Supplemental Figure S5B). We then tested whether the mutant SSRP1 that lacks the C-terminal region is able to rescue the CENP-A incorporation defect observed in SSRP1-depleted cells. The SSRP1 conditional mutant cells that stably express wild-type or C-terminal truncated SSRP1 were established, and the efficiency for centromeric targeting of newly synthesized CENP-A was determined. We found that CENP-A-GFP was not efficiently targeted to centromeres in the cells expressing SSRP1 mutant that lacks C-terminal region (Supplemental Figure S5C). These data suggest that C-terminal region of SSRP1 plays an important role for targeting of CENP-A to centromeres.
SSRP1 Is Not Required for Centromeric Localization of the CENP-H–containing Complex
To confirm that FACT is required for incorporation of nascent CENP-A, we examined whether SSRP1 depletion affected the centromeric localization of the CENP-H-containing complex. We performed immunostaining with antibodies raised against CENP-H, CENP-K and CENP-C in SSRP1 conditional mutant cells cultured in the absence or presence of tet. We found that the centromeric localization of CENP-H, CENP-K and CENP-C was not altered at interphase or mitosis at 45 h after the addition of tet (Figure 5). We conclude that depletion of FACT does not affect the centromeric localization of the CENP-H–containing complex.
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CHD1 Localizes to Centromeres through Interaction with SSRP1
FACT alters chromatin structure without using ATP hydrolysis (Ruone et al., 2003; Rhoades et al., 2004). This alteration to chromatin structure may require an additional chromatin remodeling factor because FACT can displace a histone H2A/H2B dimer from a nucleosome but does not have remodeling activity against a histone H3/H4 tetramer. The chromatin remodeling factor CHD1 was reported to interact directly with SSRP1 in human, Drosophila, and yeast cells (Kelley et al., 1999; Simic et al., 2003). Hrp1, the Schizosaccharomyces pombe orthologue of CHD1, is involved in the maintenance of the level of SpCENP-A at centromeres (Walfridsson et al., 2005). Thus, it is possible that CHD1 is involved in CENP-A deposition into centromeres in concert with FACT and the CENP-H–containing complex.
As a first step to examine this possibility, we investigated whether chicken CHD1 physically interacts with FACT. SSRP1 conditional mutant cell lines that stably express SSRP1-GFP and CHD1-FLAG were established and cultured in the presence of tet. These cell lines grew continuously in the presence of tet, indicating that the GFP-tagged SSRP1 was functional. Immunoprecipitation was performed using an anti-GFP antibody, and CHD1-FLAG was detected using an anti-FLAG antibody. We found that CHD1-FLAG coimmunoprecipitated with SSRP1-GFP when both proteins were coexpressed (Figure 6A).
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The biochemical association of CHD1 with SSRP1 suggests that CHD1 might function at centromeres together with FACT. Therefore, we analyzed whether CHD1 localizes to centromeres. Immunostaining was performed using anti-CHD1 and anti-CENP-A antibodies in SSRP1 conditional mutant cells. The cells were cultured in the absence or presence of tet for 45 h, treated with 0.1% Triton X-100 to eliminate soluble proteins, and then fixed with paraformaldehyde. We found that CHD1 localized to centromeres at interphase and mitosis in cells cultured in the absence of tet. However, CHD1 disappeared from centromeres under SSRP1 depleted conditions (Figure 6D). These results indicate that FACT is required for the centromeric localization of CHD1.
As is shown in Figure 2, the CENP-H–containing complex is required for the centromeric localization of FACT. To confirm that the CENP-H–containing complex is also required for the centromeric localization of CHD1, we performed immunostaining of CHD1 in CENP-K and CENP-C conditional mutant cells that stably expressed CENP-A-RFP as a centromeric marker. When these cells were cultured in the presence of tet, the centromeric localization of CHD1 was abolished in CENP-K conditional mutant cells (Figure 6D), whereas the depletion of CENP-C did not affect the centromeric localization of CHD1 (Supplemental Figure S8). These results suggest that the CENP-H–containing complex is required for centromeric targeting of both CHD1 and FACT.
CHD1 Is Required for Centromeric Localization of CENP-A
To determine whether CHD1 is involved in the centromeric localization of CENP-A, RNA interference (RNAi) knockdown was performed using HeLa S3 cells. The amount of CHD1 protein was reduced to an undetectable level by 72 h after the transfection of CHD1 specific siRNAs (Figure 7A). We used RT-PCR to confirm that expression of the CENP-A gene was not affected by the CHD1 siRNA treatment (Figure 7B). Next, we examined the effect of CHD1 depletion on the level of endogenous CENP-A localization at centromeres. Cells were cultured for 72 h after the transfection of CHD1-specific siRNAs or control siRNAs, and CENP-A was visualized with a specific antibody. In this time period, cells divided approximately three times by judging from cell number counting (Supplemental Figure S9). We found that the level of endogenous CENP-A localized at centromeres was significantly reduced after the CHD1 specific siRNA treatment (Figure 7C). CENP-A signals at centromere loci (n = 100) were quantified using MetaMorph software (Molecular Devices, Sunnyvale, CA). The intensities of the CENP-A signals in CHD1-depleted cells were reduced to 15% of those in cells treated with the control siRNA (Figure 7D). The reduction in the amounts of CENP-A was further confirmed by Western blot analysis of purified chromatin from CHD1-depleted or control cells. The amount of CENP-A associated with chromatin in CHD1-depleted cells fell to 22% of that in the control cells (Figure 7E). These results suggest that CHD1 is required for incorporation of CENP-A into centromeric chromatin.
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DISCUSSION |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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/β, and M18BP1/KNL2 are involved in loading of CENP-A into centromeres in vertebrates and nematodes (Hayashi et al., 2004; Fujita et al., 2007; Maddox et al., 2007). HJURP interacts directly with CENP-A and is required for centromeric deposition of CENP-A (Foltz et al., 2009; Dunleavy et al., 2009). Remodeling and spacing factor complex mediates stable association of CENP-A to the centromeric chromatin (Perpelescu et al., 2009). However, the additional molecular mechanisms for this process have not yet been determined. We previously identified the CENP-H–containing complex as a member of the CCAN in chicken and human cells and reported that the CENP-H–containing complex is required for the efficient targeting of newly synthesized CENP-A into centromeres (Okada et al., 2006). However, it was unclear how the CENP-H–containing complex is involved in the deposition of nascent CENP-A into centromeres. In this study, we demonstrate that the chromatin remodeling complex FACT and the ATP-dependent chromatin remodeling factor CHD1 are localized at centromeres in the CENP-H–containing complex dependent manner and are required for CENP-A incorporation into centromeres. How does the CENP-H–containing complex act to incorporate CENP-A into centromeric chromatin? We propose a model for deposition of nascent CENP-A at centromeric chromatin, in which FACT and CHD1 may be directly involved in CENP-A deposition. The CENP-H–containing complex constitutively localizes to centromeres and is required for the centromeric localization of FACT and CHD1. After DNA replication, the level of pre-existing CENP-A–containing nucleosomes is halved in each sister chromatid. CHD1 deposits nascent CENP-A to centromeric chromatin and thereby replenishes the level of CENP-A in centromeric chromatin.
We have shown that newly synthesized CENP-A is not efficiently incorporated to centromeric chromatin in the SSRP1-depleted cells. As is shown in Figure 3, SSRP1 knockout cells stop cell cycle progression without arrest at any particular cell cycle stage shortly after SSRP1 depletion. Thus, we are unable to exclude the possibility that the absence of nascent CENP-A incorporation in the SSRP1-depleted cells may be caused by the arrest in cell cycle before the time window of CENP-A incorporation. However, our data reveal that FACT plays a fundamental role for nascent CENP-A incorporation to centromeric chromatin at least through recruiting CHD1 to centromeres.
We speculate that centromerically localized FACT might participate in the incorporation of CENP-A by changing chromatin structure. FACT binds to histones H2A and H2B (Orphanides et al., 1999) and destabilizes chromatin structure without using ATP hydrolysis (Ruone et al., 2003; Rhoades et al., 2004). Displacement of H2A/H2B dimers is thought to facilitate remodeling of histone H3 molecules, because H3 molecules are physically masked by H2A/H2B dimers in the complete nucleosome structure (Luger et al., 1997). Indeed, we previously showed that FACT facilitates loading of histone H3.3 to pre-existing nucleosomes adjacent to FACT binding sites (Shimojima et al., 2003; Nakayama et al., 2007). It is therefore possible that FACT may change the structure of centromeric chromatin to facilitate the replacement of canonical histone H3 molecules with histone H3 variants; this would help CHD1 to deposit CENP-A.
We believe that an alternative model may also be feasible. Histone H3-containing nucleosomes are interspersed with CENP-A nucleosomes in centromeric chromatin (Blower et al., 2002). We recently reported that the CENP-T and CENP-W proteins of the CCAN associate with H3-containing nucleosomes at the centromeric chromatin (Hori et al., 2008). Centromeric localization of CENP-T/W is upstream of the CENP-H–containing complex, suggesting a close relationship between CENP-T/W and the CENP-H–containing complex. It is therefore possible that the CENP-H–containing complex might associate with the H3-containing nucleosomes at centromeres and enhance CENP-A incorporation through its effect on chromatin structures surrounding the CENP-A–containing nucleosomes. In fission yeast, FACT is involved in the formation of centromeric heterochromatin (Lejeune et al., 2007), and centromeric heterochromatin is required for the incorporation of SpCENP-A to centromeres on naked templates (Folco et al., 2008), suggesting that maintenance of the structural integrity of centromeric chromatin is important for deposition of CENP-A into centromeres.
In vertebrate cells, the CENP-H–containing complex, FACT and CHD1 are localized at centromeres throughout the cell cycle, whereas CENP-A is incorporated into centromeres from telophase to early G1. One possible mechanism for achieving cell cycle dependent CENP-A loading is by the hMis18 complex functioning in concert with the CENP-H–containing complex. hMis18 and M18BP1/KNL2 transiently localize to centromeres in the same time window as CENP-A incorporation. CENP-A mislocalization in hMis18-depleted cells is rescued by trichostatin A, suggesting that the hMis18 complex might alter the chromatin state or modulate the activity of proteins that are involved in CENP-A deposition, such as the CENP-H–containing complex, FACT or CHD1, in a cell cycle-dependent manner.
In conclusion, we show here that the CENP-H–containing complex is required for the centromeric localization of the chromatin remodeling complex FACT and of the ATP-dependent chromatin remodeling factor CHD1. In addition, we show that these chromatin remodeling enzymes are required for the centromeric localization of CENP-A. Our data suggest that the CCAN proteins play the central role in the establishment of centromere-specific chromatin structures through interaction with chromatin remodeling enzymes.
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ACKNOWLEDGMENTS |
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Footnotes |
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Address correspondence to: Masahiro Okada (maokada{at}tmu.ac.jp).
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REFERENCES |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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