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Vol. 20, Issue 23, 4941-4950, December 1, 2009
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Laboratory of Molecular Neurobiology, Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan
Submitted March 11, 2009;
Revised September 8, 2009;
Accepted September 25, 2009
Monitoring Editor: J. Silvio Gutkind
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ABSTRACT |
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 TOP ABSTRACT INTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONACKNOWLEDGMENTS START HEREREFERENCES |
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INTRODUCTION |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONACKNOWLEDGMENTS START HEREREFERENCES |
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). In early stages of cortical development, the VZ is abundant in neural progenitor cells (NPCs), whose proliferative activity is spatially and temporally controlled by various molecules including cell cycle regulators, transcription factors, and extracellular factors (Götz and Huttner, 2005; Dehay and Kennedy, 2007). To date, a large number of studies have shown the process of neurogenesis and the molecular involvement in it, but intracellular signal transduction pathways regulating NPC proliferation remain largely unknown.
The Rho family small GTPases have been already known to be involved in various cellular functions such as cell migration, neurite extension, cell cycle progression, and cell division (Etienne-Manneville and Hall, 2002; Jaffe and Hall, 2005). Like other small GTPases, Rho family GTPases act as molecular switches by cycling between an inactive GDP-bound state and an active GTP-bound state, and activated GTPases can interact with their specific effectors. Rho family GTPases have been also suggested to be involved in various stages of nervous system development including neuronal migration, neurite outgrowth, axon guidance, and synaptogenesis (Luo, 2000; Negishi and Katoh, 2002; Govek et al., 2005). In the past few years, several lines of in vivo analyses have revealed the involvement of Rho family GTPases in early cortical development. For example, experiments using in vivo electroporation with constitutively active or dominant negative mutants show that Rac1 and Cdc42 are involved in radial migration of cortical neurons (Kawauchi et al., 2003; Konno et al., 2005). An analysis of Rac1 conditional knockout mice shows that Rac1 controls the formation of midline commissures and the competency of tangential migration in ventral telencephalic neurons, and the mice also show the neural progenitor reduction and microcephaly (Chen et al., 2007, 2009). On the other hand, two distinct analyses of Cdc42 conditional knockout mice show that Cdc42 regulates NPC fate at the apical surface (Cappello et al., 2006) and Cdc42 deficiency causes holoprosencephaly (Chen et al., 2006). In addition, Rnd2, a member of Rho family GTPases that is predominantly expressed in brain, regulates the migration and morphological changes of cortical pyramidal neurons (Nakamura et al., 2006; Heng et al., 2008).
RhoG, a member of Rho family GTPases, was originally identified as a product of growth-stimulated genes in fibroblast (Vincent et al., 1992). Previous studies have shown that RhoG is involved in diverse cellular functions by regulating cytoskeletal reorganization in various types of cells, including the regulation of neurite outgrowth in neuronal cells (Katoh et al., 2000, Katoh and Negishi, 2003), cell migration (Hiramoto et al., 2006; Katoh et al., 2006a, Meller et al., 2008), macropinocytosis in fibroblasts (Ellerbroek et al., 2004), and phagocytosis of apoptotic cells (deBakker et al., 2004; Nakaya et al., 2006). In addition, RhoG is also involved in Ras-induced focus formation (Roux et al., 1997), gene expression (Vigorito et al., 2003), and cell survival (Murga et al., 2002; Yamaki et al., 2007). Northern blot analyses have shown that RhoG is expressed in the various tissues (Vincent et al., 1992) including the brain during development (Ishikawa et al., 2002). However, little has known about the physiological functions of RhoG.
In this study, we attempted to reveal the role of RhoG in brain development, particularly focusing on the cerebral cortex. Because RhoG was expressed in the developing VZ, which is abundant in NPCs, we utilized in vivo electroporation to examine the NPC-specific functions of RhoG and showed a novel function of RhoG in the regulation of NPC proliferation. These observations contribute in part to the understanding of the physiological role of RhoG and the molecular mechanisms underlying cortical development.
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MATERIALS AND METHODS |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONACKNOWLEDGMENTS START HEREREFERENCES |
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) was used as a control. Myc-tagged wild-type RhoG (RhoG-WT), RhoG-V12, and RhoG-V12A37 generated previously (Katoh et al., 2000) were inserted into pCAG-EYFP-CAG vector (Saito and Nakatsuji, 2001). pCAG-EYFP-U6 vector was constructed by inserting a fragment encoding the human U6 promoter into pCAG-EYFP (Iwasato et al., 2007). The short hairpin RNAs (shRNAs) for RhoG were designed to target 19 nucleotides of the mouse RhoG transcript (shRG-A: nucleotides 48-66, 5'-GACGTGCCTTCTCATCTGC-3' and shRG-B: nucleotides 94-112, 5'-TACATCCCCACTGTGTTTG-3'). A control shRNA for luciferase was designed as described previously (Fujito et al., 2005; Yamaki et al., 2007). These plasmids have CAG promoter that is efficient to introduce genes into mammalian cells (Niwa et al., 1991). pEF-BOS-Myc-RhoG-WT vector generated previously (Katoh et al., 2000) is used for the rescue experiment.
Antibodies
We used the following primary antibodies: rabbit polyclonal anti-GFP (1:1000, Molecular Probes, Eugene, OR), rabbit polyclonal anti-Ki67 (1:1000, Abcam, Cambridge MA), mouse monoclonal anti-Nestin (1:500, BD PharMingen), mouse monoclonal anti-BrdU (1:2000, BD Biosciences Pharmingen, San Diego, CA), mouse monoclonal anti-Tuj1 (1:3000, Covance, Madison, WI), mouse monoclonal anti-Myc (1:500, Santa Cruz Biotechnology, Santa Cruz, CA), mouse monoclonal anti-
-tubulin (1:1000, Sigma, St. Louis, MO), rabbit polyclonal anti-phospho Akt (Ser473) (1:1000, Cell Signaling Technology, Beverly, MA), and Alexa Fluor 488–conjugated anti-GFP (1:1000, Molecular Probes); and the secondary antibodies: Alexa Fluor 488–conjugated goat anti-rabbit IgG (1:1000, Molecular Probes), Alexa Fluor 555–conjugated goat anti-mouse IgG (1:3000, Molecular Probes), Alexa Fluor 594–conjugated goat anti-mouse IgG (1:3000, Molecular Probes), Alexa Fluor 594–conjugated goat anti-rabbit IgG (1:1000, Molecular Probes), Alexa Fluor 647–conjugated goat anti-rabbit IgG (1:1000, Molecular Probes), horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (1:3000, DAKO, Carpinteria, CA), and HRP-conjugated goat anti-rabbit IgG (1:1000, DAKO).
Tissue Preparation
Pregnant ICR mice were purchased from Japan SLC (Shizuoka, Japan), and treated in accordance with the guidelines for the Animal Care and Use Committee of the Graduate School of Biostudies at Kyoto University. Isolated embryonic day 12 (E12) embryos and brains were fixed in 4% paraformaldehyde (PFA) in phosphate-buffered saline (PBS) and then saturated with 30% sucrose in PBS overnight at 4°C. Embryos and brains were embedded in OCT compound (Sakura, Torrance, CA), frozen with dry ice, and stored at –80°C until use.
In Situ Hybridization
To obtain a specific riboprobe for mouse RhoG, the RhoG cDNA fragment corresponding to the nucleotide 1–400 was generated from mouse brain total RNA by using standard RT-PCR method (primers, 5'-TTTCTAGAATGCAGAGCATCAAGTGTGT-3' and 5'-TTCTCGAGGACCCTGCTCCTTGAGGCGC-3') and subcloned into pBluescript SK(+). The nucleotide sequence was confirmed using the ABI Prism 310 Genetic Analyzer (Applied Biosystems, Foster City, CA). Antisense and sense probes were synthesized in vitro transcription with T7 and T3 RNA polymerases from the XbaI- and XhoI-digested plasmid, respectively, and digoxigenin (DIG)-labeled by DIG RNA-labeling mix (Roche, Indianapolis, IN).
In situ hybridization was performed as described previously (Katoh et al., 2006b). In brief, 40-µm-thick coronal sections were treated with 0.5 µg/ml proteinase K (Roche) for 3 min and then acetylated in acetic anhydride/triethanolamine-HCl for 10 min at room temperature. After prehybridization in hybridization buffer (50% deionized formamide, 5x SSC, 5x Denhardt's solution, 250 µg/ml salmon sperm DNA, and 250 µg/ml yeast tRNA) for 2 h, the sections were hybridized overnight at 55°C with the DIG-labeled antisense or sense probe. After washing, the sections were blocked with 1% blocking reagent (Roche) for 1 h at room temperature and incubated with alkaline phosphatase–conjugated anti-DIG antibody (1:2000, Roche) overnight at 4°C. Antibody binding was detected with 5-bromo-4-chloro-3-indolylphosphate and nitroblue tetrazolium (Roche).
In Vivo Electroporation
In vivo electroporation was performed as described previously (Saito and Nakatsuji, 2001). In brief, timed pregnant ICR mice were deeply anesthetized, and the uterine horns carrying embryos were exposed through a midline abdominal incision. Two microliters of plasmid solutions (0.5–0.8 µg/µl diluted with saline) was injected into the lateral ventricle of the embryos using a micropipette made from a glass capillary. Electric pulses (50 ms at 950-ms intervals) were delivered five times with forceps-type electrodes (CUY650P5, Nepa Gene, Chiba, Japan) and an electroporator (CUY21EDIT, Nepa Gene) at 40 V for E13 or 50 V for E14. The uterine horns were then placed back into the abdominal cavity and the abdominal wall was sutured.
Immunohistochemistry and Immunocytochemistry
Frozen brains were sliced in 10-µm-thick coronal sections using a cryostat (CM3050S, Leica, Deerfield, IL). Sections were washed with PBS, permeabilized with 0.3% Triton X-100 in PBS for 15 min, and then blocked with PBS containing 1% blocking reagent (Roche), 1% goat serum, and 0.15% Triton X-100 for 1 h at room temperature. The sections were incubated with the primary antibodies for 24 h at 4°C. After five washes with PBS containing 0.1% Tween 20, the sections were incubated with the appropriate secondary antibodies for 24 h at 4°C. Then they were washed five times with PBS containing 0.1% Tween 20 and mounted using 90% glycerol containing 0.1% p-phenylenediamine dihydrochloride in PBS.
Cultured cells on coverslips were fixed with 4% PFA in PBS for 20 min and washed three times with PBS. The cells are permeabilized with 0.2% Triton X-100 in PBS for 10 min and blocked with 10% fatal bovine serum (FBS) in PBS for 1 h. Then they were incubated with primary antibodies overnight at 4°C, washed five times with PBS, and incubated with appropriate secondary antibodies. After five washes with PBS, the cells were treated with Hoechst 33258 for 5 min and mounted. Images were captured using a Nikon Eclipse E800 microscope and objectives equipped with a Leica DC350F digital camera (Melville, NY), and processed by ImageJ (http://rsb.info.nih.gov/ij/) and Photoshop (Adobe Systems, San Jose, CA).
BrdU Labeling and TUNEL Assay
For in vivo labeling, pregnant mice were injected intraperitoneally with BrdU dissolved in saline at 50 µg/g of body weight. The mice were either killed 30 min or 2 h after the injection for analysis of BrdU incorporation. For cell cycle exit analysis, the mice were killed 24 h after injection. For in vitro labeling, BrdU was added at a final concentration of 3 µg/ml to the medium, and cells were incubated for 3 h. To identify BrdU-incorporated cells, sections and cells were pretreated with 4N HCl in PBS for 10 min at room temperature before the application of anti-BrdU antibody. TUNEL (TdT-mediated dUTP-biotin nick end labeling) assay was performed using in situ cell death detection kit TMR red (Roche) according to the manufacturer's instruction. As a positive control, sections were pretreated with 10 U/ml DNase I for 10 min at room temperature.
NPC Primary Culture
Timed pregnant mice were killed, and embryos were removed. Embryonic cerebral cortices were dissected in ice-cold calcium- and magnesium-free HBSS. After incubated in HBSS with 0.25% trypsin and 0.1% DNase for 15 min at 37°C, the tissues were triturated mechanically with a pipette and dispersed in single cell in DMEM containing 4 mM L-glutamine, 100 U/ml penicillin, 0.1 mg/ml streptomycin, 1% B27 supplement (Invitrogen), 1% N2 supplement (Invitrogen), and 10 ng/ml basic FGF (Sigma). The cells were plated onto round 13-mm coverslips coated with 0.01 mg/ml poly-D-lysine at a density of 2 x 105 cells and cultured in the medium under the humidified conditions in 95% air and 5% CO2 at 37°C. For the phosphatidylinositol 3-kinase (PI3K) inhibitor treatment, the medium was changed to fresh medium containing LY294002 (Calbiochem, La Jolla, CA) 3 h after plating.
Detection of Akt Phosphorylation
After E12 brains were dissected and NPCs were isolated, NPCs (3 x 106 cells) were electroporated before plating with mouse NSC nucleofector kit (Amaxa Biosystems, Gaithersburg, MD) according to the manufacturer's instructions. Electroporated cells were cultured in the DMEM/F12 (1:1, GIBCO, Rockville, MD) containing 1% N2 supplement, 10 ng/ml basic fibroblast growth factor (FGF), and 10 ng/ml epidermal growth factor (EGF) for 20 h under the humidified conditions in 95% air and 5% CO2 at 37°C. Cells were then collected and lysed with 1x Laemmli sample buffer. The cell lysates were analyzed by SDS–PAGE and immunoblotting. Densitometry analysis was performed with ImageJ.
HEK293T Cell Culture and Transfection
HEK293T cells were cultured in DMEM containing 10% FBS, 4 mM L-glutamine, 100 U/ml penicillin, and 0.1 mg/ml streptomycin under the humidified conditions in 95% air and 5% CO2 at 37°C. HEK293T cells were transfected with indicated expression vectors using Lipofectamine Plus (Invitrogen), according to the manufacturer's instructions.
Immunoblotting
Proteins were separated by SDS-PAGE and were electrophoretically transferred onto a polyvinylidene difluoride membrane (Millipore, Bedford, MA). The membrane was blocked with 3% low fat milk in Tris-buffered saline, and then incubated with primary antibodies. The primary antibodies were detected with HRP-conjugated secondary antibodies and a chemiluminescence detection kit (Chemi-Lumi One; Nacalai Tesque, Kyoto, Japan; ECL Plus Western Blotting Detection System; GE Healthcare, Waukesha, WI).
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RESULTS |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONACKNOWLEDGMENTS START HEREREFERENCES |
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). No significant signal was detected in the section hybridized with a sense probe (Figure 1A, right). Focusing on the cerebral cortex, RhoG mRNA was detected in the VZ and the subventricular zone (SVZ) from E12 to E16 but neither in the preplate (PP), the IZ nor the CP at E12 and E14, although it was weakly detected in the CP at E16 (Figure 1B). These results suggest that RhoG is expressed in the proliferative VZ/SVZ at early stages of cortical development.
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, 2006a; Estrach et al., 2002; Katoh and Negishi, 2003). However, the expression of RhoG-V12A37 also increased the percentage of BrdU-incorporated cells at a level similar to that of RhoG-V12-expressing cells (Figure 2, C and D). Therefore, these results indicate that activation of RhoG promotes NPC proliferation through a pathway independent of the ELMO-mediated activation of Rac1.
Knockdown of RhoG Reduces Cultured NPC Proliferation and BrdU Incorporation
To evaluate the involvement of intrinsic activity of RhoG in NPC proliferation, we performed RNA interference (RNAi)-mediated knockdown of RhoG. To visualize the shRNA-expressing cells, we used a double promoter vector, which expressed both enhanced yellow fluorescent protein (EYFP) and shRNA in the same cells (Figure 3A). We designed two shRNAs for two different regions of mouse RhoG cDNA (shRG-A and -B), and used a luciferase shRNA as a control shRNA (shLuc). To check the effectiveness of RhoG protein knockdown by these two shRNAs, Myc-tagged mouse RhoG and each shRNA expression vectors were coexpressed in HEK293T cells. These two shRNA expression vectors for RhoG effectively reduced the amount of exogenously expressed Myc-tagged mouse RhoG in HEK293T cells, although shRG-B is less effective than shRG-A (Figure 3B). We could not examine the effect of the shRNAs on endogenous RhoG protein because we could not obtain antibodies for detecting specifically endogenous RhoG.
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RhoG Regulates NPC Proliferation In Vivo
Next, to test in vivo requirement of RhoG activity on NPC proliferation, cortical sections of electroporated brains were stained with an antibody against the nuclear factor Ki67, which is expressed in proliferating cells from S- through M-phase. There was a significant increase in the percentage of Ki67+ cells in RhoG-V12–electroporated brains compared with that in control brains (Figure 4, A and B). On the other hand, there was a significant decrease in the percentage of Ki67+ cells in both shRG-A– and -B–electroporated brains compared with that in shLuc-electroporated brains (Figure 4, C and D). In addition, the reduction in the percentage of Ki67+ cells by shRG-A electroporation was rescued by coelectroporation with hRhoG-WT, which was resistant to the mouse RhoG shRNA (Figure 4, C and D). These results suggest that RhoG regulates NPC proliferation in vivo.
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; Yamaki et al., 2007). These observations indicate that RhoG is involved in cell survival. Therefore we examined whether or not the suppression of proliferation and BrdU incorporation of NPCs by knockdown of RhoG was due to the induction of apoptosis. Apoptotic cell death was detected by TUNEL assay. We performed the experimental validation by pretreating with DNase I as a positive control (Figure 5C). As a result, few TUNEL+ cells were observed in the brain electroporated with RhoG shRNAs as well as with control luciferase shRNA (Figure 5, A and B), suggesting that suppression of proliferation and BrdU incorporation of NPCs by knockdown of RhoG is not due to the induction of apoptosis.
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80% of the cells were Nestin+ at E14 (Figure 6B), and when they were subsequently cultured in vitro, the percentage of Nestin+ cells gradually decreased because Nestin+ NPCs differentiated into neurons (Figure 6, A and B). Instead, most of the control cells were positive for a neuronal marker Tuj1 at 48 h after plating (Figure 6, C and D). However, the percentage of Nestin+ cells in RhoG-V12–electroporated cells remained significantly higher than that in the control cells at 48 h after plating (Figure 6, A and B). In contrast, the percentages of Tuj1+ cells remained lower (Figure 6, C and D). In addition, we frequently observed Nestin+ or Tuj1– cell clusters in RhoG-V12-electroporated cells (Figure 6, A, arrows, and C, arrowheads). On the other hand, expression of RhoG-WT or knockdown of RhoG did not significantly affect the percentages of Nestin+ or Tuj1+ cells (Figure 6, A–F). To further examine the involvement of RhoG in NPC differentiation, we determined the fraction exiting cell cycle in shRNA-electroporated brains referred to in the previous study by Sanada and Tsai (2005). Brains were electroporated with shRNA expression vectors at E14, pulse-labeled by BrdU at E15, and stained with antibodies against EYFP, BrdU, and Ki67 at E16. We estimated the fraction exiting the cell cycle representing the percentage of EYFP+BrdU+Ki67– cells in EYFP+BrdU+ cells. However, no significant difference is observed in the fraction exiting the cell cycle between control- and RhoG-shRNAs-electroporated brains (Figure 6G). Therefore, these results suggest that RhoG is not involved in the maintenance of undifferentiated state of NPCs and that the higher percentage of Nestin+ cells by expression of RhoG-V12 may be due to the expansion of highly proliferative NPCs.
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; Yamaki et al., 2007). As shown above, RhoG-V12A37 promoted BrdU incorporation (Figure 2, C and D). RhoG-V12A37 is mutated in the effector domain of RhoG-V12 and cannot bind to ELMO, but can interact with PI3K and induce PI3K-mediated Akt phosphorylation (Yamaki et al., 2007). Therefore, we investigated whether PI3K activity was required for the regulation of NPC proliferation by RhoG. RhoG-V12-electroporated cells were labeled with BrdU in the absence or presence of a PI3K inhibitor LY294002. The increase in the BrdU-incorporated cells by RhoG-V12 was suppressed by treatment with LY294002 (Figure 7A). In addition, the RhoG-V12–induced increase in the percentage of Nestin+ cells was also significantly suppressed by LY294002 treatment (Figure 7B). To examine whether RhoG triggers the activation of the PI3K pathway in NPCs, they were transfected with control or RhoG-V12 vector and phosphorylation of Akt was detected by immunoblotting. Expression of RhoG-V12 significantly increased the level of phosphorylated Akt (Figure 7C). Thus, these results indicate that regulation of NPC proliferation by RhoG is mediated by PI3K activity.
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DISCUSSION |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONACKNOWLEDGMENTS START HEREREFERENCES |
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Previous studies using cell lines have provided some evidence that RhoG is involved in cell proliferation. In NIH3T3 cells, expression of constitutively active RhoG increases cell saturation density, and dominant negative RhoG-expressing cells increase doubling time at first and then grow normally, but stop growing at lower saturation density. Moreover, constitutively active RhoG potentiates Ras-induced focus formation (Roux et al., 1997). Another study has shown that RhoG-depleted HeLa cells by RNAi are less proliferative (Yamaki et al., 2007). On the other hand, the previous pathological study showed that RhoG is highly expressed in human breast cancer, and it is correlated with tumor malignancy (Jiang et al., 2003). In addition, a comprehensive analysis of Rho family GTPases has shown that RhoG mRNA is highly expressed in mouse embryonic stem (ES) cells (Boureux et al., 2007). Both tumor cells and ES cells possess highly proliferative activity. Thus, the expression level of RhoG may be correlated with the proliferative activity.
Previous studies have provided the knowledge about signaling pathways downstream of RhoG. It has been known that RhoG activates Rac1 and Cdc42 through distinct pathways and regulates actin cytoskeleton (Gauthier-Rouviére et al., 1998; Wennerberg et al., 2002). We have previously shown that RhoG activates Rac1 and promotes neurite outgrowth and cell migration by direct interaction with its specific effector ELMO and a Rac-specific guanine nucleotide exchange factor (GEF), Dock180 (Katoh and Negishi, 2003; Katoh et al., 2006a). The RhoG-ELMO-Dock180–mediated activation of Rac1 is also involved in phagocytosis of apoptotic cells (deBakker et al., 2004), and recent studies have shown that this pathway is utilized by bacterial invasion (Patel and Galán, 2006; Handa et al., 2007; Roppenser et al., 2009). However, our present study has shown that the ELMO-Dock180–mediated activation of Rac1 is dispensable for the promotion of NPC proliferation by RhoG because overexpression of an effector domain mutant of RhoG that cannot activate Rac1 also promoted BrdU incorporation at the level comparable to that of overexpression of constitutively active RhoG. Thus, Rac1 does not appear to be required for the RhoG-mediated NPC proliferation. On the other hand, RhoG interacts with the PI3K regulatory subunit, p85, and induces phosphorylation of Akt. This pathway is involved in the resistance of UV-induced apoptosis and anoikis, a form of apoptosis induced by cells detaching from extracellular matrix (Murga et al., 2002; Yamaki et al., 2007). In the present study, we show that a PI3K inhibitor LY294002 blocked the promotion of NPC proliferation by RhoG. Thus, these results suggest that RhoG regulates NPC proliferation via PI3K but not Rac1. From our results, LY294002 had little effect on the BrdU incorporation in control-electroporated cells, suggesting that this signaling pathway is not activated in NPCs in the culture condition. When LY294002 was intraventricularly injected, however, the percentage of BrdU+ cells in the LY294002-injected brains was significantly reduced compared with that in the control brains (data not shown). This result indicates that PI3K activity is required for NPC proliferation in vivo.
PI3K is one of the key molecules that regulate cell proliferation in normal development and tumorigenesis. PI3K phosphorylates phosphatidylinositol-4,5-diphosphate and produces phosphatidylinositol-3,4,5-triphosphate, which activates Akt. PI3K/Akt signaling regulates cell proliferation through various kinds of signaling pathways (Vivanco and Sawyers, 2002; Takahashi et al., 2005; Engelman et al., 2006). For example, Akt promotes the G1/S transition by blocking FOXO-mediated transcription of cell cycle inhibitors. Akt also induces cell proliferation by mTOR/p70S6K/4E-BP–mediated protein synthesis. Another study showed that Akt can indirectly stabilize the cell cycle proteins such as c-Myc and cyclin D1 by inhibiting GSK3. Recently, Mairet-Coello et al. (2009) showed that PI3K/Akt activation by IGF-1 increased cyclin E and decreased p27Kip1 and p57Kip2 expression in cortical progenitors, suggesting that PI3K/Akt controls G1/S cell cycle progression in NPCs by regulating the transcription of cell cycle–related genes. In the present study, we showed that RhoG promotes NPC proliferation through PI3K pathway, and knockdown of RhoG results in a decrease in the percentage of BrdU+ as well as Ki67+ cells. On the other hand, the decrease in proliferation by knockdown of RhoG is neither due to the induction of apoptosis nor differentiation to the neuron. Taken together, RhoG may regulate G1/S cell cycle progression by modulating the transcription of cell cycle–related genes, and the decrease in proliferating NPCs upon RhoG knockdown may be due to delayed entry into the next cell cycle caused by slow progression of S-phase or the cell cycle arrest.
In the present study, we could not refer to the upstream regulators of RhoG in NPC proliferation. Currently, several molecules including SGEF, Trio, Kalirin, Vav, and PLEKHG6 are identified as GEFs for RhoG (Schuebel et al., 1998; Movilla and Bustelo, 1999; Blangy et al., 2000; May et al., 2002; Vigorito et al., 2003; Ellerbroek et al., 2004; D'Angelo et al., 2007). Although Kalirin is expressed predominantly in the nervous system and initiates new axon outgrowth mediated by RhoG in superior cervical ganglion neurons (May et al., 2002), it is not expressed in the VZ during early cortical development (Hansel et al., 2001). On the other hand, the regulatory mechanism of expression of RhoG is also largely unknown. However, given that RhoG was originally identified in fibroblast as a gene that is transcribed in a serum or growth factor stimulation–dependent manner (Vincent et al., 1992), expression of RhoG may be spatially and/or temporally regulated by extracellular factors such as growth factors in the developing cerebral cortex, and may be linked to the cell cycle events. Our present study provides a novel physiological function of RhoG and a new aspect of the regulation of cortical development. However, there are still several questions that remain to be resolved, including how RhoG-PI3K signaling regulates cell proliferation, and that GEF modulates RhoG activity during corticogenesis. Further investigations are required for understanding the regulation of RhoG signaling during cortical development.
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Footnotes |
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Address correspondence to: Hironori Katoh (hirokato{at}pharm.kyoto-u.ac.jp).
Abbreviations used: BrdU, bromodeoxyuridine; CP, cortical plate; IZ, intermediate zone; NPC, neural progenitor cell; PI3K, phosphatidylinositol 3-kinase; shRNA, short hairpin RNA; SVZ, subventricular zone; VZ, ventricular zone.
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