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Vol. 20, Issue 7, 2083-2095, April 1, 2009
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*Department of Biochemistry and Department of Biological Sciences, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117456, Singapore; Departments of Biochemistry, and ¶Molecular Biology, University of Geneva, CH-1211 Geneva, Switzerland; Institute of Molecular Biotechnology, Graz University of Technology, A-8010 Graz, Austria; ||Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima 739-8528, Japan; and #Extremobiosphere Research Center, Japan Agency for Marine-Earth Science and Technology, Yokosuka 237-0061, Japan
Submitted November 17, 2008;
Revised January 28, 2009;
Accepted February 5, 2009
Monitoring Editor: Benjamin S. Glick
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ABSTRACT |
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 TOP ABSTRACT INTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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INTRODUCTION |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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). Cholesterol has been shown to modulate membrane thickness in artificial membranes, and this property has been proposed to play a role in membrane protein localization in vivo (Bretscher and Munro, 1993) although an alternative explanation for the control of membrane thickness has been postulated (Mitra et al., 2004). Recently it has been shown that proteins and lipids do not freely diffuse over the entire surface of the cell, and it has been proposed that eukaryotic plasma membranes contain micro/nanodomains (for review see Simons and Ikonen, 1997; Kusumi et al., 2005; Jacobson et al., 2007). Indeed, different hypotheses exist to explain domain formation. The raft hypothesis has two basic tenets (Simons and Ikonen, 1997): 1) that sterols and sphingolipids interact specifically in biological membranes and 2) that their interaction causes an increase in membrane lipid order, creating membrane microdomains that affect protein function, diffusion, and/or localization. To our knowledge, there is currently a lack of evidence to demonstrate a specific role of lipid order in biological membranes as postulated by the raft hypothesis. Another hypothesis, known as the lipid shell hypothesis, suggests that domains are formed through association of specific lipids, in particular sterols and sphingolipids, with proteins (Anderson and Jacobson, 2002).
There is clear biophysical evidence that sterols and sphingolipids can segregate from other lipids in simple artificial membrane systems to form liquid ordered domains (Ahmed et al., 1997). Sterol partitioning experiments between membranes in vitro also suggest that they have affinity for membranes with a high content of sphingolipids (Wattenberg and Silbert, 1983). Sterols have been shown to have a condensing effect on artificial membranes (Radhakrishnan and McConnell, 2005), and sterol-sphingomyelin–condensed complexes have been characterized (Radhakrishnan et al., 2001). Some evidence exists in yeast suggesting a genetic interaction between mutants in sterol and sphingolipid biosynthesis (Baudry et al., 2001; Eisenkolb et al., 2002; Jin et al., 2008); however, little, if any, convincing evidence exists to show that these two lipid species function together in complex biological membranes. Therefore, the basic question of whether sterols and sphingolipids interact functionally and preferentially in biological systems remains unsolved and is a major focus of this study.
Nevertheless, various lines of evidence suggest a possible link between these two lipid classes. Sterols and sphingolipids are concomitantly affected in certain diseases. In Niemann Pick disease, although the primary defect is not yet completely certain, defects in sphingolipid and cholesterol trafficking appear to be interdependent (Puri et al., 1999; Pagano et al., 2000; Vance, 2006). One of the proposed functions of amyloid beta and presenilin is in control of sphingomyelin and cholesterol amounts in the brain (Grimm et al., 2005), which could affect the ontology of Alzheimer's. Sphingolipid depletion has also been shown to influence the SREBP pathway, controlling cholesterol and lipid biosynthesis (Scheek et al., 1997).
In this study, we combine quantitative lipid analysis and genetics in an unbiased approach to study yeast mutants in sterol synthesis. This systematic analysis provides evidence that yeast cells have a mechanism to respond to the presence of specific sterol structures in their membranes and adjust their sphingolipid composition accordingly. The genetic analysis provides convincing evidence that sterols and sphingolipids function together in cells to carry out multiple functions. Furthermore, our results suggest that sterol and sphingolipid structures have coevolved to provide optimal interaction properties between the two lipid species. Our results support the assertion of the raft hypothesis that sterols and sphingolipids interact preferentially in biological membranes; however, our initial studies of plasma membrane fluidity suggest that the increased order that can be induced by sterol–sphingolipid interaction is not the most important factor for any of the several physiological readouts we have investigated.
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MATERIALS AND METHODS |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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) was used to fluorescently tag Pdr12p in the genome using plasmid pBS10 (Yeast Resource Center, University of Washington). Yeast were grown in rich medium (1% yeast extract, 2% peptone, 2% glucose, and 40 mg/l uracil and adenine) to log phase for lipidomic and sterol analysis. Forty OD600 units of cells were harvested by centrifugation, washed with water, and frozen. For the plating assays, yeast were grown to stationary phase and diluted to 107 cells per ml in water, and 10-fold serial dilutions were pinned onto agar plates containing rich medium with additives adjusted to pH 5.5 (40 mM MES) unless indicated. For Western blot analyses, proteins were extracted after trichloroacetic acid denaturation as previously described (Urban et al., 2007). Phospho-Ypk1 and phospho-Sch9 were detected using an anti-serum described in Wanke et al. (2008). Anti-Ypk1 antiserum was purchased from Santa Cruz Biotechnology (Santa Cruz, CA).
Lipid Standards
All lipid standards were obtained from Avanti Polar Lipids (Alabaster, AL), with the exception of C19:0-ceramide and dioctanoyl glycerophosphoinositol (GPIns), which were obtained from Matreya (Pleasant Gap, PA) and Echelon Biosciences (Salt Lake City, UT), respectively.
Lipid Analysis by Electrospray Ionization–Mass Spectrometry
Lipids were extracted from 40 OD600-equivalents of cells and as described (Guan and Wenk, 2006), with the following modifications. Five micrograms of dimyristoyl glycerophosphocholine (GPCho), 20 µg of dimyristoyl glycerophosphoethanolamine (GPEtn), 4 µg of dioctyl GPIns, 15 µg of didocosahexaenoyl glycerophosphoserine (GPSer), and 3 µg of C19:0-ceramide were added as internal standards. Quantification of individual molecular species was carried out using multiple reaction monitoring (MRM) with a 4000 Q-Trap mass spectrometer (Applied Biosystems, Foster City, CA). Typically 25 µl of samples was injected for analysis. The inlet system consisted of an auto-sampler and a pump, and chloroform–methanol (1:1, vol/vol) at a flow rate of 200 µl/min was used as the mobile phase. In these experiments, the first quadrupole, Q1, was set to pass the precursor ion of interest to the collision cell, Q2, where it underwent collision-induced dissociation. The third quadruple, Q3, was set to pass the structure-specific product ion characteristic of the precursor lipid of interest. Each individual ion dissociation pathway was optimized with regard to collision energy to minimize variations in relative ion abundance due to differences in rates of dissociation (Guan and Wenk, 2006). Lipid levels were calculated relative to relevant internal standards. Comparison of the means of wild-type and individual genotypes from three independent experiments were performed. The quantities of lipids are expressed as ion intensities relative to wild-type levels, converted to a log10 scale. The difference in levels of individual lipid species between wild-type and individual genotypes was determined statistically using the Kruskal-Wallis test.
Sterol Analysis by Gas Liquid Chromatography–Mass Spectrometry
Four micrograms of cholesterol standard were added to 40 A600 equivalents of washed cells, and total sterols were extracted three times with petrol ether after alkaline hydrolysis as described previously (Munn et al., 1999; Heese-Peck et al., 2002). Total sterol extracts were taken up in 10 µl pyridine and silylated using 10 µl of BSTFA (Sigma, St. Louis, MO). For gas-liquid chromatography–mass spectrometry (GC-MS) samples were diluted in ethyl acetate and analyzed on an HP 5890 Series II gas chromatograph (Hewlett-Packard, Palo Alto, CA) equipped with an HP 5972 mass selective detector and an HP 5-MS column (cross-linked 5% phenyl methyl siloxane; dimensions 30 m x 0.25 mm x0.25-µm film thickness). Aliquots of 1 µl were injected in the splitless mode at 270°C injection temperature with helium as carrier gas at a flow rate of 0.9 ml/min in constant flow mode. The temperature program was 1 min at 100°C, 10°C/min to 250°C, and 3°C/min to 310°C. Mass spectra were acquired in scan mode (range, 200–550 atomic mass units) with 3.27 scans per second. Sterols were identified based on their mass fragmentation pattern and retention time relative to the cholesterol standard (Mullner et al., 2005).
Fluorescence Anisotropy Measurements
Cells were grown in YPD medium at 30°C to 1–5 x 107 cells/ml. After two washes (10 mM Tris-Cl, 1 mM EDTA, pH 7.0), they were labeled with 0.5 µM of 1-[4-(trimethylamino)pheny]-6-phenyl-1,3,5-hexatriene (TMA-DPH; Invitrogen, Carlsbad, CA) at 25°C for 10 min. The sur2 strains were labeled with 2 µM TMA-DPH. After two washes, the cells were placed in a RF-5300PC spectrofluorometer (Shimadzu, Kyoto, Japan) fixed with a polarization unit. The excitation and emission wavelengths were chosen at 358 and 458 nm and measured 25°C. Anisotropy (r) was calculated from polarized intensities according to the equation:
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Fluorescent Microscopy Analysis
Yeast strains with plasmid pRS416-TAT2-mRFP (generated by subcloning the HindIII-XbaI fragment from pMO10 (Okamoto et al., 2006) into pRS416 were grown to log phase at 25°C in synthetic minimal medium lacking uracil and tryptophan. TAT2- monomeric red fluorescent protein (mRFP) was observed with a fluorescence microscope (BX51; Olympus, Tokyo, Japan). The fluorescence intensity of TAT2-mRFP in the plasma membrane and vacuole was quantified by a Slide Book 4 Digital Microscopy software (Intelligent Imaging Innovations, Denver, CO) and expressed as a percentage of total intensity. The data are shown as means ± SDs from n = 27–59 cells. Yeast strains with plasmid Can1-green fluorescent protein (GFP) were grown in minimum media at 30°C to exponential phase (OD600 = 0.7) and resuspended in potassium phosphate buffer (pH 7.0). Live specimens were visualized using Zeiss 510 Meta confocal microscope (Thornwood, NY) with a 100x PlanApochromat objective (excitation 488 nm). Image processing was done with Image J (http://rsb.info.nih.gov/ij/). To examine patches on the plasma membrane Z-stacks were created to generate images of entire cells and confocal sections of the cell surface were visualized (data not shown). Fluorescence images of Pdr12-CFP were all acquired using the same exposure time.
Transcript Analysis
Yeast strains were grown in triplicate in exactly the same conditions (unique batch of YPUAD, same day) until an OD600 of 0.8–1.0 was reached. Two ml of culture was centrifuged; cells were frozen in liquid N2 and stored at –80°C. Total RNA extraction was performed using the RNeasy Mini Kit (No. 74104; Qiagen, Chatsworth, CA) mechanical disruption protocol including a column DNAse treatment.
RNA purity was controlled on a spectrophotometer; the ratios A260/A280 (protein contamination) and A260/A230 (guanidinium thiocyanate contamination) were more than or equal to 2.0. RNA integrity control was performed by capillary electrophoresis on an Agilent Technologies 2100 Bioanalyzer (Wilmington, DE).
Five hundred nanograms of RNA was amplified and labeled using MessageAmp II-Biotin Enhanced (No. AM1791; Ambion, Austin, TX). Biotin-labeled cRNA, 20 µg, was fragmented and hybridized for 16 h to Affymetrix GeneChip Yeast2.0 Array (Affymetrix, Santa Clara, CA). The hybridization and development with streptavidin was performed using GeneChip Hybridization Wash and Stain Kit (No. 900720; Affymetrix). Washing and development of the chip was done on GeneChip Fluidics Station 450. Three biological replicates were processed for each strain.
Microarray Analysis
Summarization and normalization (scaling) of the microarray data were conducted using GCOS (Affymetrix). The software package GeneSpring GX 7.3 Analysis Platform (Agilent Technologies) and Matlab 2008 (MathWorks, Natick, MA) were then used to analyze the microarray data. Microarray expression ratios were presented as log-transformed base 2. Hierarchical clustering analysis focused on transcript expression patterns rather than absolute values and the triplicate data over each strain was averaged using the mean in the log2 transform matrix. The average of each strain (i.e., every probeset) was then subtracted from the averaged value of the wild-type strain in the gene expression matrix. The rows were then clustered using a correlation similarity metric and the average linkage method in Matlab.
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RESULTS |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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; Heese-Peck et al., 2002). We noticed that the double erg3 erg6 mutant, which has a substantial growth defect, showed changes in its sphingolipid metabolism (Supplementary Figure S2) and that this change could be reversed by mutation of the yeast sphingomyelinase homolog (Sawai et al., 2000) encoded by ISC1 (Figure 1). The mutation in ISC1 aggravated the growth defect of the erg3 erg6 double mutant at 37°C (Supplementary Figure S2). These data suggest a functional interaction between sterols and sphingolipids, but this particular case might not be representative. Therefore, we systematically examined the lipid composition of all of our erg mutants using electrospray ionization–mass spectrometry (ESI-MS) techniques. The erg mutants (Supplementary Table S1) were grown in rich medium to log phase, harvested, washed, and frozen. Internal standards for quantitation were added, and the lipids were extracted and quantified by ESI-MS (Guan and Wenk, 2006). The quantification of the major glycerophospholipids (except for cardiolipin) and several of the major sphingolipids are presented. Results on mannosyldiinositolphosphorylceramide (M(IP)2C) are not presented because the data were not easily interpretable because of the presence of singly and doubly charged species, together with the fact that no appropriate internal standard was available. By steady-state phosphate or inositol labeling and thin-layer chromatography analysis, M(IP)2C represents 
20% of the total sphingolipids in Saccharomyces cerevisiae (Schorling et al., 2001). Quantification was carried out relative to wild-type cells and is presented in Figure 2. The single erg mutants showed substantial changes (>30%) in <10% of their glycerophospholipid species, but showed substantial changes in more than 45% of their sphingolipid species. Remarkably, the sphingolipid pattern found in each single erg mutant was different. Therefore, the changes cannot be due to a lack of ergosterol because none of the mutant strains have ergosterol, but should be due to the presence of the abnormal sterols. This astounding result implies that yeast cells have the capacity to modify their sphingolipid composition in response to changes in sterol structures in their membranes. Many of the sterols accumulated in the mutants represent natural biosynthetic intermediates of ergosterol biosynthesis and are also found at low levels in wild-type cells (Supplementary Table S2). It is unlikely that cells are reacting to the concentration of sterols in the membrane because the total amount of sterols present in the single erg mutants was always higher than in the wild-type (Supplementary Table 2).
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; Supplementary Table S2E), explaining why the erg6 mutant has partial properties of a double erg2 erg6 mutant. Consistent with the radioactive labeling experiments, most of the double mutants as well as the erg2 and erg6 strains have less of the major inositolphosphoceramide (IPC) species, IPC-C. Otherwise, the patterns of sphingolipid prevalence vary considerably. Interestingly, when mutations in sphingolipid hydroxylation or head group turnover are introduced, there are no major changes in sterol compositions or quantities (Supplementary Table S2A). Most double erg mutants show more substantial changes in their sphingolipid patterns than either of the corresponding single erg mutants. There is one notable exception to this, the erg5 erg6 mutant, whose sphingolipid pattern is closer to wild type than either of the two single mutants. This might have evolutionary significance (see Discussion).
Sterol and Sphingolipid Biosynthesis Pathways Interact Genetically
The specificity of the sphingolipid patterns in the different single erg mutants suggests that these changes may be a physiological response to accumulation of sterol biosynthetic intermediates and led us to hypothesize that sterols and sphingolipids functionally interact in yeast cells. To test this hypothesis, we created isogenic double mutants in sterol and sphingolipid biosynthesis. The prediction is that simultaneous defects in both pathways should lead to synthetic phenotypes. The changes in sphingolipid patterns (Figure 2) are mainly associated with changes in sphingolipid hydroxylation or in head group composition. Therefore, we chose a mutant that affects sphingolipid head group turnover, isc1, and mutants affecting sphingolipid hydroxylation. The sur2 mutant is unable to hydroxylate the sphingoid base and the scs7 mutant is defective in hydroxylation of the fatty acid on sphingolipids (see Figure 1 and Supplementary Figure S1; Haak et al., 1997). We made all 15 possible double mutant combinations of the five single erg mutants, with the three mutants affecting sphingolipid metabolism, and analyzed their ability to grow under a number of conditions. All of the single erg mutants, with or without sphingolipid defects, grew well at 30°C on rich medium, as do all of the double erg mutants except erg2 erg6 (Figure 3). At 37°C or on plates with nonfermentable carbon sources, several double mutants showed reduced growth when compared with the corresponding single mutants (marked with arrows). The double erg mutants notably exhibited more severe phenotypes than the single erg mutants from which they were derived.
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), whereas both corresponding single mutants grow well on caffeine plates. A similar result was found for sensitivity to rapamycin (see below). Combination of sur2 with erg3 or erg6 led to synthetic growth phenotypes on plates with a low concentration of a glycosylphosphatidylinositol synthesis inhibitor, YW3548 (Sutterlin et al., 1997) or a low concentration of SDS (Figure 4C, left and right, respectively). These conditions are both expected to induce cell wall defects. The specificity of the site of hydroxylation in combination with particular sterol biosynthesis mutations suggest that it is the combination of the structures that leads to the synthetic phenotype. The simplest way to interpret this would be to postulate that sphingolipids and sterols interact physically in membranes to carry out some of their required functions. At the very least, we can conclude that they work in common pathways.
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), which exports weak acids such as sorbic and benzoic acids. This process is most affected by the erg3 and erg6 mutations (Supplementary Figure S3), suggesting that a 
5 double bond in the B-ring and methylation of the side chain of ergosterol are the most important features for Pdr12p function. The erg4 mutant strain grows weakly on 1 mM sorbic acid plates, but combination with the sur2 mutation completely abrogates growth (Figure 4D). Introduction of the isc1 mutation improves growth slightly under the same conditions. The effects of sphingolipid changes on the erg5 mutant are quite different. The sur2 and scs7 mutations have almost no effect on growth of erg5 cells on sorbic acid plates, whereas introduction of the isc1 mutation completely blocks growth under the same conditions. These data show that the isc1 mutation is not having a simple positive or negative effect that is added to the effect of the sterol mutant. These results further confirm that it is the specific combinations of sphingolipid and sterol structures that lead to the synthetic phenotypes. By using very simple tests we found synthetic phenotypes in 13 of the 15 possible double mutants in ergosterol and sphingolipid biosynthesis (Figure 3), providing genetic proof that these two pathways work together to carry out multiple cellular functions. A possible explanation for the genetic interaction could be that creation of the double mutants causes additional changes in glycerophospholipids and/or sterols. To examine this, we analyzed the glycerophospholipids, sphingolipid, and sterol compositions in the double mutant strains by ESI-MS. As expected from the introduction of mutations in sphingolipid modification, we observed dramatic changes in the sphingolipid pattern, but relatively minor changes in the phospholipids or sterols (Supplementary Figure S4 and Supplementary Table S2), lending additional support to our conclusion that it is the structural interactions between the sterols and sphingolipids in the membrane that cause the synthetic phenotypes and not further changes in phospholipids or sterols.
In this study, we have established that sterols and sphingolipids function together. We next sought to characterize the lipid dependence of two phenotypes to demonstrate how the combination of sterols and sphingolipids can affect the manner in which cells respond to drugs. The first example is the hypersensitivity of sterol–sphingolipid mutants to caffeine and rapamycin, and the second example is the hypersensitivity to sorbic acid.
Reduced TORC2 Activity Causes Caffeine and Rapamycin Hypersensitivity
TORC1 is the target of caffeine and rapamycin in yeast both in vivo and in vitro (Wanke et al., 2008), whereas TORC2 is not significantly inhibited by either drug. Inhibition of TORC1 yields profound changes in the transcriptome, including a striking down-regulation of genes encoding ribosomal proteins (Martin et al., 2004; De Virgilio and Loewith, 2006). Therefore, we first examined expression of ribosomal protein mRNAs in our different strains to determine if reduced TORC1 activity could explain the increased caffeine sensitivity. Levels of mRNAs encoding ribosomal proteins were not affected in any of the mutants where transcript profiles were examined, including erg2 scs7 cells (see data at ArrayExpress at EBI, Experiment Riezman, Accession no. ID E-TABM-544). Surprisingly, when genes that were specifically overexpressed in the erg2 scs7 mutant were examined, we found RTA1, a gene that seems to be repressed by TORC2 (Mulet et al., 2006). On further inspection, a tight cluster of genes that are negatively regulated by TOR2 was found to be overexpressed in the erg2 scs7 mutant specifically (see Figure 7 and Supplementary Figure S5), suggesting that TORC2 might be less active in the mutant. This is the case because Ypk1p, one of the two Ypk kinases directly phosphorylated by TORC2 (Kamada et al., 2005; M. Picolis and R. Loewith, unpublished data) is hypophosphorylated in the mutant (Figure 5A). Sch9p, a direct TORC1 target (Urban et al., 2007), is phosphorylated normally in these strains, confirming that TORC1 is active. These results and the previous observation that ypk1 mutant cells are hypersensitive to rapamycin (Gelperin et al., 2002) suggest that reduced TORC2 activity and hypersensitivity to caffeine and rapamycin might be related. To investigate this, we introduced a plasmid carrying a constitutively active Ypk2 allele (Kamada et al., 2005) to bypass TORC2 function into the set of erg2 and scs7 mutant strains. The results were very clear. Ypk2 kinase activity suppressed the hypersensitivity of the erg2 scs7 strain to caffeine and rapamycin (Figure 5B). These results demonstrate that combination of the erg2 and scs7 mutations renders yeast cells hypersensitive to caffeine and rapamycin by lowering TORC2 activity. Strikingly, the sterol–sphingolipid composition affects how cells respond to these two drugs, not by affecting the direct target of the drug, but by affecting a partially overlapping pathway.
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) in an ATP-dependent manner. To examine Pdr12p localization and function, we constructed a C-terminal genomic fusion creating a functional, fluorescently labeled Pdr12::CFP. Sorbic acid induces Pdr12p expression, and the protein is delivered to the cell surface (Piper et al., 1998) where it is the major, if not only protein that exports weak organic acids, including sorbic and benzoic acids as well as fluorescein (Holyoak et al., 1999). In wild-type, erg4, sur2, and erg4 sur2 mutants, Pdr12-CFP was induced and transported to the cell surface in approximately equal amounts as determined by fluorescence microscopy (Figure 6A). Pdr12p activity was assayed by measuring export of fluorescein. Fluorescein diacetate was loaded into yeast in the absence of glucose, conditions where export is defective. Glucose was added to allow fluorescein export. The double erg4 sur2 mutant showed drastically reduced fluorescein export, approximately six times less efficient than wild-type cells (Figure 6B). These results show that the MDR-like protein, Pdr12p, is properly transported to the cell surface, but is not active if membranes lack functional sterol–sphingolipid structures. In this example, sterol–sphingolipid composition seems to affect the drug export protein in a fairly direct manner.
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300 nm on the cell surface that contain the various permeases, including the tryptophan transporter Tat2p and the arginine transporter Can1p. Both transporters have been reported to be resistant to cold-detergent extraction, a technique that is often correlated with presence in rafts (Malinska et al., 2003, 2004; Umebayashi and Nakano, 2003; Mayor and Riezman, 2004). The permease-containing plasma membrane patches also stain for filipin, indicating the presence of ergosterol (Malinska et al., 2003, 2004). More recently, it has been shown that many genes, including some involved in sterol metabolism, affect the protein composition and structure of these patches (Grossmann et al., 2008). The permease-containing patches are surrounded by membrane domains containing the plasma membrane ATPase, a protein that has also been claimed to be functionally associated with rafts for biosynthetic delivery to the cell surface (Bagnat et al., 2000; Bagnat and Simons, 2002). Therefore, we analyzed the localization of fluorescently tagged Tat2p and Can1p, proteins found in the same domains at the cell surface. In wild-type cells under our conditions, Tat2p is found in approximately equal amounts in the plasma membrane and in the vacuole. The vacuolar Tat2p may be generated by direct targeting from the biosynthetic pathway or via delivery to the cell surface and subsequent endocytosis. Cell surface and vacuolar Tat2-GFP in the various mutants were quantified (Supplementary Figure S5; Table 1). The single erg mutants as well as isc1 and sur2 increased the amount of Tat2-GFP in the vacuole, and several strains showed slight endoplasmic reticulum (ER) accumulation. The increase in vacuole localization in the erg3 mutant is partially suppressed by the scs7 mutation, whereas the increase in vacuolar localization in the erg6 mutant is enhanced by isc1 and sur2 mutations. This demonstrates suppression and synthetic effects also for protein localization. On the other hand, the effects on Can1-GFP localization are only minor. In all mutants we detected the majority of Can1-GFP at the plasma membrane (Supplementary Figure S5). In the erg2 and erg3 series we found a small amount of vacuole staining, but no synthetic phenotypes (Table 1). The Can1-GFP construct was active in all strains because it conferred canavanine sensitivity. Therefore, two proteins localizing to the same plasma membrane patches seem to have quite different sterol and sphingolipid requirements.
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). It will be of interest to determine if the localization of TORC2 to this domain is specifically altered in erg2 scs7 cells.
Because most of the plasma membrane surface is covered by proteins that have been proposed to localize to rafts, we decided to measure the fluorescence anisotropy of the plasma membrane using TMA-DPH. This fluorescent probe inserts into the yeast plasma membrane and measurement of its anisotropy correlates with membrane rigidity or lipid order (Sharma, 2006). The lower the anisotropy is, the more fluid is the membrane. Many of the erg mutants showed significantly and substantially lower anisotropy than wild-type cells, in particular, erg6 (Table 2). Interestingly, all of the double mutants with erg6 showed increased anisotropy, whereas they showed a greater defect in Tat2p surface localization and frequently exhibited more severe phenotypes in other assays performed in this study (Supplementary Figure S3). These results indicate that changes in sterol and sphingolipid composition definitely do affect membrane fluidity; however, there was a lack of correlation between the magnitude of these changes with any other phenotypic parameter that we have measured. From this systematic and unbiased approach to examine a role for membrane order on processes that depend upon the sterol and sphingolipid composition, we have to conclude that the fluidity/rigidity (order) of the membrane is not the major determinant in the phenotypes that we measured. This calls into question whether the lipid order predicted to be induced by sterol–sphingolipid interactions in the plasma membrane plays an important physiological role.
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DISCUSSION |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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), because sterol intermediates, rather than the total quantity of sterols, seem to affect sphingolipid composition in different ways and the changes are unlikely brought about directly through changes in gene expression. Recently, an SREBP-dependent pathway has been described in Schizosaccharomyces pombe, which has several features in common with what we have seen here, because there is a coordinate regulation of anaerobically expressed genes with ergosterol biosynthesis genes (Todd et al., 2006; Figure 7) and because sterol biosynthesis intermediates seem to be important (Hughes et al., 2007), but no specific effects on sphingolipids have been described. The pathway cannot be identical in S. cerevisiae because this yeast does not have an SREBP homolog, although they do have oxysterol-binding proteins that have been proposed to be involved in intracellular sterol transport (Raychaudhuri et al., 2006).
An interesting question is the physiological significance of the adaptation mechanism. First, it is likely that natural intermediates in the ergosterol biosynthesis pathway are being recognized. They are present in significant amounts under normal growth conditions and are found in larger amounts in the erg mutants (Supplementary Table S2). Sterol biosynthetic intermediates have been shown to be localized preferentially to the ER membrane (Zinser et al., 1993), and their amounts could be important in regulating adaptation mechanisms. Second, yeast may frequently encounter azole and other inhibitors of ergosterol biosynthesis in their natural environment and therefore require adaptation mechanisms to adjust their sphingolipid composition when azole compounds are present and inhibit sterol biosynthesis at various steps.
The control of sphingolipid species is unlikely to be solely the result of transcriptional changes because few differences in sphingolipid metabolic enzymes were observed. In particular, some changes in sphingolipid content are likely to be due to head group turnover via Isc1p (Supplementary Figure S2). The mode of activation of Isc1p in this case is unknown, but one possibility is regulation by localization because its localization has been shown to change as a result of changes in culture conditions in yeast (Vaena de Avalos et al., 2004).
In contrast to the effect of sterol mutations on sphingolipid composition, mutations affecting hydroxylation and head group turnover of sphingolipids do not seem to affect the sterol composition. Therefore, this process is not reciprocal. There could be different explanations for this situation. First, the species of sphingolipids that change in the sterol mutants are sphingolipid species that are normally present in substantial quantities, and only their amount changes. They cannot all be considered as intermediates, because each of these sphingolipid species might be functional. Known antifungals that target the sphingolipid biosynthesis pathway act at or before the step of inositolphosphorylceramide (IPC) synthesis (Horvath et al., 1994; Mandala and Harris, 2000; Sugimoto et al., 2004). In contrast, the major sterol species is ergosterol, and other species are probably just intermediates in its biosynthesis. Second, during evolution some metazoans either never had or lost the ability to synthesize sterols and are sterol auxotrophs (Vinci et al., 2008). Two particularly well-studied systems are Drosophila and Caenorhabditis elegans (Silberkang et al., 1983; Matyash et al., 2001). Experiments with Drosophila did not detect any major changes in phospholipid head groups or acyl group composition when grown with different amounts of cholesterol (Silberkang et al., 1983). Sphingolipids were not examined in this study. No information is published yet from either system about the lipid composition of these organisms when they are grown using different sterols. It is possible that these organisms have a mechanism similar to that described here for yeast that could be used to adjust membrane composition in response to which sterols they ingest.
The specific and major changes in sphingolipid species when sterol intermediates are present in the membrane show a dependence of sphingolipid metabolism on sterol composition, but also suggest that sterols and sphingolipids function together. The second major finding from our study stems from our genetic analysis of double mutants in sterol and sphingolipid metabolism and provides proof for their functional interaction. This study revealed a number of novel synthetic and suppression phenotypes between sterol and sphingolipid biosynthesis mutants. Two aspects of this systematic and unbiased analysis are particularly convincing. First is the penetration of phenotypes, with 13 of the 15 possible double mutants showing synthetic or suppression phenotypes. Second is the astounding specificity of the combinations required to affect each phenotype and the strict specificity for the site of hydroxylation on the sphingolipid molecule and its combination with a particular sterol mutation. Synthetic phenotypes can occur for different reasons: when there are two parallel pathways and one mutation is in each pathway or when two mutations affect the same pathway, quite often at the same step. Each mutation would affect the step partially, but the double mutant would have a much stronger phenotype. Classically, this can result when two proteins work together as a complex. In a similar manner it is possible to interpret our data by suggesting that sterols and sphingolipids might function in a variety of pathways as a sterol–sphingolipid complex. Sterols and sphingolipids are clearly capable of forming condensed complexes in artificial membranes (Radhakrishnan et al., 2000), and there is no reason to expect that they should not be able to do the same in biological membranes.
We tested many different phenotypes including growth in presence of cell wall disturbances, weak organic acid sensitivity, different carbon sources, temperatures, various classes of inhibitors affecting a number of different pathways as well as a systematic analysis of transcript levels. It is virtually impossible that sterols and sphingolipids happen to act in parallel pathways to carry out each of these functions where synthetic phenotypes have been uncovered. Therefore, we are obliged to conclude that sterols and sphingolipids function together to carry out a wide variety of cellular functions. The conclusion that sterols and sphingolipids function together is consistent with one of the tenets of the raft hypothesis. The other main tenet of the raft hypothesis is that the increased order produced by sterol–sphingolipid interactions is important for function. To begin to test this, we measured plasma membrane anisotropy as an indicator of membrane order. Even though there were substantial and significant differences in anisotropy, indicating a decrease in membrane order in several of the mutants, we were not able to correlate the measurements of membrane anisotropy with any of the phenotypes we assayed, and therefore we conclude that membrane order is not likely to have a great influence on the functions we examined. Therefore, our results do not provide support for this part of the raft hypothesis.
The raft hypothesis has been invoked to explain a large number of membrane trafficking events, which prompted us to examine the localization of two plasma membrane proteins that have been shown to be localized in microdomains of the plasma membrane. Here again, the raft hypothesis does not easily help to explain the results of steady-state localization of Tat2p and Can1p in the various single and double mutants. The two transporters colocalize to the same plasma membrane patches, but they show completely different dependence on sterols and sphingolipids for their localization. Localization of Tat2p, which was affected by sphingolipid and sterol mutations, did not correlate with anisotropy measurements. It could be that anisotropy measurements of the plasma membrane do not reflect fluidity of the compartment where Tat2p sorting occurs (Golgi or endosomal compartment) or that measuring the overall fluidity of the membrane does not indicate the fluidity in rafts, so we cannot disprove involvement of liquid order phases. On the other hand, there is no reason from our studies or others in the literature at this time to invoke a role for liquid order phases in vivo.
In what ways could sterol–sphingolipid interactions influence function with such a complex pattern of phenotypes? A trivial explanation would be that addition of sterol mutations with sphingolipid mutations cause an indirect effect by generally increasing the stress to the cells, which affects many functions. This is not the case for the hypersensitivities seen here to caffeine or sorbic acid, which do not correlate with stress gene induction. Indirect effects, although certainly occurring in some cases, are unlikely to be a common explanation for the synthetic effects we see because of the specificity of the position of hydroxylation of the sphingolipid and because different combinations of specific sterol and sphingolipid mutations affect only specific cellular pathways rather than having highly pleiotropic effects.
Biophysical (Ahmed et al., 1997; Radhakrishnan et al., 2001; Feigenson, 2007) and molecular dynamics (Aittoniemi et al., 2007) experiments have clearly demonstrated that sterols and sphingolipids can interact preferentially in artificial membranes. This means that these lipids exist in at least two forms in the membrane, free sterol and sphingolipid, as well as sterol–sphingolipid complexes. Our results suggest that this interaction will also be seen in vivo if suitable techniques become available. We postulate that most of the phenotypes seen here are mainly the result of defects in direct protein–lipid interactions and not changes in the fluidity properties of the membrane or membrane domains. Any integral or peripheral membrane protein could interact with either a sterol, a sphingolipid, or a sphingolipid-sterol complex, helping to explain the complexity of the phenotypic analysis. Membrane proteins might require the sterols and/sphingolipids for proper folding, assembly, targeting, regulation, or activity. Eukaryotic membrane proteins have had a long time to optimize these interactions during evolution because most cells have sterols and all have sphingolipids. Direct sterol–sphingolipid interactions could also help to explain why it is so difficult to express, purify, and crystallize eukaryotic membrane proteins in bacteria.
There are various ways to interpret the enhancement of phenotypes we have seen. For example, in erg4 and sur2 single mutant cells Pdr12p shows substantial activity, but in erg4 sur2 double mutants activity is lost. Pdr12p might require interaction with sterol–sphingolipid complexes for full activity. The interaction could be reduced because of a stronger change in shape of the complex due to the combination. The complex might adapt a different tilt in the membrane because of the two mutations. Molecular dynamics studies suggest that sphingomyelin binding to cholesterol controls its tilt in the lipid bilayer (Aittoniemi et al., 2006). Alternatively, the combination of the erg4 and sur2 mutations could affect the equilibrium between the free lipids with sterol–sphingolipid complexes affecting Pdr12p activity. We propose that this paradigm—of protein interaction with sterols, sphingolipids, or their complexes—could explain the majority of phenotypes we see and that have been shown to depend upon sterols or sphingolipids in the literature without invoking the need for order in the membranes.
In order for sterols and sphingolipids to function together, their structural compatibility had to be maintained through evolution. Strikingly, there was one outlier from the lipid analysis of the double erg mutants (Figure 2). The sphingolipid pattern in the erg5 erg6 double mutant was more similar to wild type than the corresponding single mutants. The double mutation is "sphingolipid neutral." The ERG5 and ERG6 genes are responsible for changes in the sterol side chain, the principle difference in the biosynthetic pathways of cholesterol and ergosterol. The two genes are linked in S. cerevisiae. This suggests that the ERG5 and ERG6 genes could have been inherited or lost together during evolution without causing major sphingolipid changes, allowing a coevolution of sphingolipids and sterols.
This study also demonstrates that changes in sterol and sphingolipid composition can affect how a cell responds to a drug or inhibitor. Several examples are shown here, most prominently, the hypersensitivity of the erg2 scs7 double mutant to caffeine and rapamycin. Our data show that the reduction in activity of TORC2, not TORC1, the direct target of caffeine and rapamycin, is the reason for the hypersensitivity. The extent to which this paradigm can be extended to humans is not presently clear, but it is evident that genetic factors and diet affect sensitivity and effects of certain drugs. Sterols and sphingolipid composition could play an important role in pharmacological variations in the population.
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ACKNOWLEDGMENTS |
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Footnotes |
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These authors contributed equally to this work. 
Co-senior authors; address correspondence to: Markus R. Wenk (bchmrw{at}nus.edu.sg) or Howard Riezman (howard.riezman{at}unige.ch)
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