INTRODUCTION

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Recent studies have indicated that the ability to respond to and recover from various stress challenges declines with age. Our studies have shown an age-associated increase in the constitutive expression of the acute phase reactant gene ₁-acid glycoprotein (AGP) and an extended lag period in its induction by bacterial LPS (Carter et al., 1991; Post et al., 1991). Other age-associated effects on stress response genes include an increase in the constitutive expression of the T-kininogen (Sierra et al., 1989) gene, failure of hsp70 genes to respond to hyperthermia due to changes in the activity of heat shock gene transcription factors (Liu et al., 1989; 1991; Choi et al., 1990; Heydari et al., 1993), and repression of the androgen receptor gene due to changes in NFB activity and induction of a repressor protein (Supakar et al., 1995). These studies suggest that the effects of aging on the regulation of families of stress response genes may be due to changes in the structure and/or function of transcription factors that regulate these families of genes. The C/EBPs are a family of trans-acting factors that play a major role in the regulation of families of stress response genes. To determine whether aging affects the structure and/or function of C/EBP and C/EBP isoforms, we studied their DNA-binding activity and protein pool levels in the mouse liver as a function of age and/or LPS treatment.

It has been proposed that the formation of multiple C/EBP and C/EBP isoforms is mediated by the differential use of multiple AUG initiation codons within the same ORF of a single mRNA (Descombes and Schibler, 1991; Lin et al., 1993; Ossipow et al., 1993; Calkhoven et al., 1994; An et al., 1996). Thus, alternative translational initiation produces 42-kDa (p42^C/EBP), 38-kDa, 35-kDa, 30-kDa, and 20-kDa (p20^C/EBP) isoforms; the C/EBP isoforms produced by this mechanism are 35 kDa (p35^C/EBP), 30 kDa (p30^C/EBP), and 20 kDa (p20^C/EBP) (An et al., 1996). These isoforms, both C/EBP and C/EBP, differ significantly in their regulatory functions. For example, p42^C/EBP is a powerful trans-activator of several genes coordinately regulated during the differentiation of preadipocytes to adipocytes and has antiproliferative activity associated with adipocyte differentiation (Umek et al., 1991; Lin et al., 1993; Lin and Lane, 1994). The p30^C/EBP, which is initiated from an AUG codon downstream of the first AUG, fails to interfere with adipocyte cell proliferation and to induce complete 3T3-L1 differentiation. The isoform also attenuates transcriptional activation by p42^C/EBP. The C/EBP isoforms include p35^C/EBP, which binds to region D of the albumin promoter and acts as a powerful trans-activator, and p20^C/EBP whose truncated transcription activation domain antagonizes p35^C/EBP activity (Descombes et al., 1990; Descombes and Schibler, 1991). Both the 35- and 20-kDa C/EBPs repress transcription of Rous sarcoma virus (Sears and Sealy, 1994); p42^C/EBP has repressor activity for human hepatitis B virus and simian virus 40 (Lopez-Caberra et al., 1990; Pei and Shih, 1990, 1991). We have shown that C/EBP is the isoform that binds to the acute phase response element (APRE) of the ₁-acid glycoprotein promoter during the LPS-mediated acute phase response (Alam and Papaconstantinou, 1992; Alam et al., 1993) and that when transcription of the AGP gene is activated, it is the 20-kDa C/EBP that binds to the APRE, suggesting that p20^C/EBP may be a trans-activator for this gene (An et al., 1996). These studies have shown that LPS stimulates a dramatic change in the isoforms of the C/EBP and C/EBP family of transcription factors (Baumann et al., 1990, 1992; Chang et al., 1990; Alam and Papaconstantinou, 1992; Alam et al., 1993; An et al., 1996). Using the APRE oligonucleotide we have demonstrated a major shift in the DNA-binding activities from C/EBP to C/EBP during the hepatic response to LPS (Alam et al., 1993; An et al., 1996). Specifically, we have shown that p42^C/EBP is the predominant isoform that binds to the APRE in nuclear extracts from untreated mice and that the p20^C/EBP becomes the major isoform in the DNA-protein complex formed with nuclear extracts from LPS-treated liver (An et al., 1996). Concomitant with the increase in C/EBP-binding activity, the binding activity of C/EBP is decreased significantly in response to LPS. Furthermore, we demonstrated that during the acute phase response specific C/EBP and C/EBP protein pool levels are up- and down-regulated, respectively; this LPS-mediated regulation of C/EBP- and C/EBP-binding activity and pool levels may play an integral role in gene regulation during the acute phase response. These observations have suggested to us that age-associated changes in the constitutive and LPS-inducible levels of C/EBP isoforms may be important factors in changes in acute phase reactant gene activity seen in aged liver.

In this article, we present our studies on the effects of aging on the DNA-binding activity and protein pool levels of C/EBP and C/EBP isoforms. On the basis of our recent results suggesting that C/EBP and C/EBP isoform synthesis is mediated by LPS treatment, and is due to alternative translation initiation at specific AUG initiation sites in the C/EBP and C/EBP mRNAs, we initiated these studies to determine whether there are age-associated changes in the regulation of alternative translational initiation that alter the differential translation of C/EBP and C/EBP mRNAs.

	MATERIALS AND METHODS

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Animals

Male C57BL/6J mice from the National Institute on Aging colonies (Bethesda, MD) were obtained through the Charles River Laboratories (Wilmington, MA). All animals were maintained on a light/dark (12-h/12-h) cycle and fed standard chow diet.

Animals were injected intraperitoneally with 10 µg of LPS (Sigma, St. Louis, MO) in pyrogen-free saline and were killed by cervical dislocation at indicated time points. In the course of our previous studies we have noted that 50 µg of LPS produce a maximal acute phase response in young mice. However, this dose of LPS causes a greater than 50% mortality for aged mice (24-28 mo). In view of this high mortality rate, we have used the lower LPS dosage in the present studies.

Preparation of Nuclear Protein Extracts

Preparation of liver nuclear protein extracts from control and LPS-treated mice has been described (Zhang et al., 1990; Alam et al., 1993). Aliquots of the nuclear protein solutions were frozen in liquid nitrogen and stored at 90°C.

Electrophoretic Mobility Shift Assays (EMSAs)

EMSAs were carried out as described by Alam et al. (1993). The following oligonucleotide, i.e., the APRE corresponding to the C/EBP-binding site of the AGP-1 promoter and its complementary strand were used as a probe for EMSA or Southwestern blot analysis after labeling with [-³²P]ATP by T4 polynucleotide kinase (Fried and Crothers, 1981; Garner and Revzin, 1981): 5'-GAACATTTTGCGCAAGACATTTCCCAAG-3'.

Equal amounts of the two complementary strands were heated at 95°C for 10 min in STE buffer (10 mM Tris-Cl, pH 8.0, 100 mM NaCl, and 1 mM EDTA) and allowed to anneal by slowly cooling to room temperature. For supershift assays, C/EBP- and C/EBP-specific antibodies were preincubated for 20 min with nuclear extracts before adding the probe. The DNA-protein complexes were resolved by electrophoresis in 6% nondenaturing polyacrylamide gels in 0.5× TBE (1× TBE: 25 mM Tris base, 25 mM boric acid, 0.5 mM EDTA).

Southwestern Blot Analysis of Nuclear Proteins Bound to DNA

Southwestern blot analysis procedures have been described by An et al. (1996).

Western Blot Analysis of Nuclear Extracts

Western immunoblot procedures have been described by An et al. (1996).

Antisera

Antisera specific for C/EBP and C/EBP were prepared against specific oligopeptides (Landschulz et al., 1988, 1989; Cao et al., 1991). The oligopeptide used to prepare the anti-C/EBP was AGPHPDLRTGGGGGGGAGA, which is adjacent to the DNA-binding domain; the C/EBP antiserum was prepared using the oligopeptide LRNLFKQLPEPLLASAG, which is at the carboxyl terminus. Antisera specific for the Flag tag sequences inserted into pCMV-C/EBP expression vectors were purchased from Eastman Kodak (Rochester, NY).

Polysome Isolation and Analysis of Binding Activity of Nascent Peptide Chains to APRE Oligonucleotide

Polysomes were isolated from control and LPS-treated mouse livers as described previously (Brown and Papaconstantinou, 1977; An et al., 1996). The protein concentration was determined according to the method of Bradford (1976). These polysomal proteins were then used in the EMSA, supershift, and Southwestern blot analyses as described.

Isolation of Proteins Binding to APRE Oligonucleotide

The DNA-protein complexes designated as C1-C5 were isolated as described by An et al. (1996).

Construction of C/EBP Expression Vectors

Amplification vector pMSV-C/EBP-SVori was constructed by cloning the PvuII/HindIII inserts of pSVori (Gluzman, 1981; Rabek et al., 1990) into the vector portion of EcoRI-digested pMSV-C/EBP (Cao et al., 1991). The identity of the clone was confirmed by restriction enzyme mapping and Southern blot analysis with ³²P-labeled SVori probe. The pCMV-C/EBP expression vectors were constructed as follows: A 1.45-kb HinfI (blunted)/BamHI restriction fragment, containing the mouse C/EBP coding region, 54 bp of 5' untranslated region and 502 bp of 3' untranslated region, was cloned into the Xba (blunted)/BamHI sites of pGEM-7zf(+) (Promega, Madison, WI), generating pGEM-7zf-C/EBP. To distinguish the protein products of expression vectors from the endogeneously generated C/EBP, the Flag tag was attached to the C-terminal of C/EBP by annealing oligonucleotides 5'-CCGAGCCGCTCGTGGCCTCGGCGGGCCACTG-3' with 5'-CAGTGGCCCGCCGAGGCCAGCAGCGGC-3'; 5'-CGACTACAAAGACGATGACGATAAATA-3' with 5'-CTAGCTATTTATCGTCATCGTCTTTGTAGTCG-3' (Flag tag codons underlined), respectively, and inserting them in AvaI/NheI sites producing pGEM-7zf-C/EBP-Flag. A XbaI/PstI restriction fragment from pGEM-7zf-C/EBP was cloned into pGEM-4z. The resultant construct was digested with BamHI/PstI to produce a BamHI/PstI restriction fragment, which along with the PstI/BamHI restriction fragment from pGEM-7zf-C/EBP-Flag were cloned into the BamHI site of pCMV-B, yielding the wild-type expression vector pCMV-C/EBP-WT. pCMV-B is a derivative from pFlag-CMV 2 (Eastman Kodak) with inactivated BamHI site and substituted SacI/HindIII sequence. Mutation of the 20-kDa AUG start site to produce pCMV-C/EBP-MT20 expression vector was prepared by site-specific mutation of the AUG  UUG in the pCMV-C/EBP-WT vector. The mutation was achieved by using the oligonucleotide primer 5'-CCGGCCGCCAAGGCGGGCGCGTCG-3' (mismatch underlined) following the method of Kunkel et al. Construction of the pAPRE-CAT expression vector has been described (Alam et al., 1993). All constructs were confirmed by sequence and restriction analysis.

Cell Culture and Transfection of C/EBP and C/EBP Standards and Expression Vectors

The procedures for the transfection of C/EBP expression vectors into COS-1 cells have been described (An et al., 1996). These nuclear proteins were used as standards in the Southwestern blot and Western blot analyses, as well as for analyses of alternative translational initiation.

	RESULTS

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Effects of Aging on Binding Activities of C/EBP and C/EBP to the APRE Oligonucleotide of the AGP-1 Promoter

Binding at the C/EBP-binding site of the AGP-1 promoter is altered during the LPS-mediated activation of the acute phase response (Alam and Papaconstantinou, 1991; Alam et al., 1993; An et al., 1996). Using the APRE oligonucleotide, we demonstrated that p42^C/EBP, p35^C/EBP, and p20^C/EBP are the isoforms in control nuclear extracts that form the APRE-protein complexes, and that p20^C/EBP, whose DNA-binding activity is minimal in controls, becomes the predominant isoform in these complexes in LPS-treated mice (Alam and Papaconstantinou, 1992; An et al., 1996). In the present experiments we conducted an EMSA time-course analysis to determine whether the complexes formed between C/EBP and C/EBP isoforms and the APRE are affected by aging. The data in Figure 1 demonstrate significant qualitative and quantitative changes in the APRE-protein complexes formed using liver nuclear extracts from control and LPS-treated 4-, 12-, 19-, and 28-mo-old mice. These experiments demonstrated the formation of four distinct APRE-protein complexes (C1, C2, C3, and C4) with nuclear extracts from untreated 4- and 12-mo-old adults (Figure 1, A and B; An et al., 1996) and that the same DNA-protein-binding pattern is formed with liver nuclear extracts from these mice (An et al., 1996). The DNA-binding pattern for both ages show a loss of the C1 and an increase in the C4 complexes at 3 h, the loss of C2-C3 complexes and further increase in intensity of the C4 complex at 6 and 12 h, and a near recovery to the control pattern at 24-48 h.

View larger version (70K): [in this window] [in a new window]   Figure 1.   A time course of DNA binding to the C/EBP-binding site (APRE) of the mouse AGP-1 promoter with nuclear proteins from livers of young and aged mice treated with LPS. EMSAs were performed using 3 µg of nuclear proteins isolated from fresh livers of control (0) or LPS-injected C57BL/6 male mice aged 4 mo (A), 12 mo (B), 19 mo (C), and 28 mo (D). LPS (10 µg) was injected at time 0, and mice were killed and nuclear proteins prepared at the times indicated at the top of each lane. The double-stranded oligonucleotide corresponding to the APRE of AGP-1 promoter was labeled with [-32P]ATP and T4 polynucleotide kinase. The DNA-protein complexes are indicated as C1, C2, C3, C4, and C5. Lane F contains the free DNA probe.

The EMSA of the nuclear extracts from aged (19- and 28-mo) control and LPS-treated animals are shown in Figure 1, C and 1D. In controls the levels of the C1 and C2 complexes are reduced, whereas the levels of the C3 (in 28 mo) and C4 (in 19 and 28 mo) complexes are increased. These experiments indicate that there is a significant increase in the ratio of C4:C1-C2 in nuclear extracts of the aged, untreated animals and suggest that these animals exhibit properties similar to those observed with LPS-treated young animals. This ratio is significantly larger if the C3-C4 complex is included in this comparison. In addition, the EMSA for the nuclear extracts of LPS-treated 28- mo-old animals show a significant increase in binding activity of the C5 DNA-protein complex (Figure 1D). Although the C5 complex is detectable in the young nuclear extracts, the dramatic increase in binding activity of this isoform appears to be an age-associated characteristic.

The EMSA time-course analyses indicate that the 4- and 12-mo-old (middle-aged) animals recover from the LPS treatment by 24 h (An et al., 1996), whereas the EMSA of the aged animals (19 and 28 mo) indicate that the DNA-protein complexes associated with the inflammatory response persist for up to 48 h (Figure 1, A versus D). Thus, in addition to exhibiting an increased binding activity indicative of an inflammatory response in the absence of a challenge, the aged animal does not recover from this inflammatory challenge as rapidly as the young adult. Studies in progress indicate that recovery occurs within 72-96 h after LPS treatment (our unpublished results).

Identification of the C/EBPs That Bind to the APRE in Control and LPS-treated Nuclear Extracts

Supershift analyses show that anti-C/EBP (Figure 2A, control lane 2) displaces the C1, C2, and some of the C3 complexes, whereas anti-C/EBP does not exhibit a strong interaction with these complexes (Figure 2A, control lane 3), but does shift the C4 and C5 complexes. Anti-C/EBP does not displace any of these complexes (our unpublished results). Analyses of the complexes formed 6 h after LPS treatment indicate that C/EBP-binding activity decreases, whereas the C/EBP-binding activity (C4 and C5) increases during the acute phase response (Figure 2A, 6 h); at 24 h after LPS treatment C/EBP has increased and C/EBP, although still significantly higher than control, has decreased from its peak (An et al., 1996). The supershift analyses indicate that in nuclear extracts of control aged mouse livers (Figure 2, B and C versus A) there is a decrease in C/EBP (C1-C3) and an increase in C/EBP binding activity (C4-C5); after 6 h of LPS treatment the difference in their binding activity increases. These data are in agreement with the EMSA data and indicate that there is an age-associated loss of C/EBP-binding activity and increase in C/EBP-binding activity, resulting in an overall increase in the ratio of C/EBP:C/EBP in control animals. Our data suggest that the C/EBP-C/EBP-binding activities are altered in aged livers, and that this may be due to changes in either their protein pool levels or DNA-binding activity.

View larger version (59K): [in this window] [in a new window]   Figure 2.   Mobility supershift analyses of the DNA-protein complexes formed with the APRE of the AGP-1 promoter. Supershift assays were done as described in MATERIALS AND METHODS to demonstrate that the nuclear proteins involved in the formation of DNA-protein complexes with the AGP-1 APRE are members of the C/EBP family. Monospecific antibodies against synthetic peptides unique to the C/EBP and C/EBP isoforms were used. Lanes F, free oligonucleotide; lanes 1, no antibody was added to the reaction mixture; lanes 2, mouse anti-C/EBP; lanes 3, mouse anti-C/EBP. Nuclear extracts were prepared 0, 6, and 24 h after LPS injection as indicated. Mice 4, 19, and 28 mo of age were used. The positions of the DNA-protein complexes are noted as C1-C5; the bands formed by supershifting with C/EBP or C/EBP antibody are indicated by the arrows. (A) 4 mo. (B) 19 mo. (C) 28 mo.

Determination of the Molecular Weights of C/EBP Proteins That Bind to the APRE

The carboxyl terminal region of C/EBP proteins contains a leucine zipper domain that facilitates formation of homo- and heterodimer (Landschulz et al., 1988). Dimerization is an essential feature of the binding activity of these proteins. In previous studies we used the APRE oligonucleotide for Southwestern blotting analyses to estimate the apparent molecular weights and binding activity of C/EBP homodimers in liver nuclear extracts of control and LPS-treated young mice (An et al., 1996). In the present studies we used this oligonucleotide to determine whether aging affects the binding activity of these C/EBPs (Figure 3). Analysis of nuclear extracts from fresh livers of control and LPS-treated, young (4-mo) and old (28-mo) mice detected bands with apparent molecular masses of 42 kDa, 35 kDa, 30 kDa, and 20 kDa in the nuclear extracts of young untreated livers (Figure 3). The nuclear extracts of aged animals showed the same molecular mass isoforms as well as the low molecular mass isoform of ~14.5 kDa which is only seen in young mice at 6 h after LPS treatment. Furthermore, the binding activity of the 42-kDa isoform decreased in the aged animal whereas the 30-kDa and 20-kDa isoforms increased. Upon LPS treatment the intensities of the 42-kDa, 35-kDa, and 30-kDa isoforms decreased or disappeared completely 6 h after treatment and reappeared after 24 h in young mice. Similarly, the high molecular mass isoforms decreased, whereas the 20-kDa and ~14.5-kDa isoforms increased in the aged (28-mo) livers in response to LPS. In general, the pattern of the C/EBP homodimer isoforms detected by Southwestern blot analyses is consistent with the pattern of binding activity demonstrated by EMSA. However, the steady-state and LPS-induced intensities of the 42-kDa, 35-kDa, and 30-kDa isoforms decreased or disappeared completely with the LPS treatment. These experiments also showed the recovery of the DNA-binding activity of the 42-kDa, 35-kDa, and 30-kDa C/EBP proteins in young nuclear extracts after 24 h, whereas this recovery is not detected in aged nuclear extracts even by 48 h. Thus, the kinetics of binding activity determined by EMSA and by Southwestern blot analyses demonstrate the same age-associated differences in C/EBP-binding activity.

View larger version (75K): [in this window] [in a new window]   Figure 3.   Southwestern blot analysis of liver nuclear proteins that bind to the APRE of the AGP-1 gene after LPS treatment. C57BL/6 mice (4- and 28-mo-old males) were injected with 10 µg of LPS and then killed 3, 6, 12, 24, or 48 h after injection. Nuclear proteins (30 µg) prepared from fresh livers were subjected to SDS-PAGE, blotted onto Westran PVDF membranes, and probed with 106 cpm/ml of 32P-labeled APRE oligonucleotide as described in MATERIALS AND METHODS. Locations of molecular size standards (kDa) are shown on the left. Lanes  and  contain nuclear extracts from COS-1 cells transfected with expression plasmids for C/EBP and C/EBP, respectively, as described in MATERIALS AND METHODS.

Age-associated Effects on the Constitutive and LPS-induced Pool Levels of C/EBP and C/EBP Isoforms

In a recent study we demonstrated by Western immunoblotting that there are multiple C/EBP and C/EBP isoforms in control 4-mo-old liver nuclear extracts and that the pool levels of these isoforms are altered by LPS treatment (An et al., 1996). The Southwestern blot analyses shown in Figure 3 indicate that there are significant qualitative and quantitative changes in the binding activity of C/EBP homodimers in nuclear extracts of both young and aged livers and in their responses to LPS. These changes may be due to either altered protein pool levels or to modification of protein structure that affects binding activity. To determine whether aging affects the C/EBP pool levels of the proteins, we assayed C/EBP and C/EBP protein levels by Western blot analyses using monospecific antibodies. As expected, anti-C/EBP recognized multiple C/EBP isoforms, with molecular masses of 42 kDa, 38 kDa, 35 kDa, 30 kDa, and 20 kDa, in control young mice (Figure 4A, 4-mo-old, lane 0). Among them the constitutive levels of the 42-kDa, 38-kDa, 35-kDa, and 30-kDa forms were high and that of the 20-kDa isoform level was low. The data also show that the protein pattern detected at 24 h after LPS treatment is similar to that of the control, indicating a recovery from the LPS. The constitutive levels of the 42- kDa, 38-kDa, and 35-kDa isoforms are dramatically reduced in the aged liver nuclear extracts (Figure 4A, 28-mo-old, lane 0), whereas the 30-kDa isoform appears to be at the same level as in the young and the 20-kDa isoform is significantly increased. Although constitutive levels of the 42-kDa, 38-kDa, and 35-kDa isoforms decreased dramatically in the aged animal, a further decrease to undetectable levels occurred after LPS treatment. At the same time, the level of the 20-kDa isoform, whose constitutive level is elevated in the uninduced aged animal, was further induced by treatment with LPS. These data show that the kinetic properties of the DNA-binding activity (Figure 1) and pool levels of p42^C/EBP isoform parallel those associated with the reduction of C/EBP DNA-binding activity in aged controls and after LPS treatment. Figure 4B shows that anti-C/EBP detects 35-kDa and 20-kDa isoforms in nuclear extracts of young control animals and recovery after 24 h. The levels of the 20-kDa and ~14.5-kDa forms were increased with the LPS treatment, and their inducibility increased with age. Thus, the homodimer-binding activities (Figure 3) and pool levels (Figure 4) follow the same pattern.

View larger version (63K): [in this window] [in a new window]   Figure 4.   Western blot analysis of the levels of C/EBP and C/EBP isoforms in liver nuclei in response to LPS treatment. Nuclear extracts (30 µg) from control (0) and LPS-injected (3, 6, 12, 24, and 48 h postinjection) C57BL/6 mice (4- and 28-mo-old males) were loaded in each lane and subjected to ECL-Western immunoblot analyses as described in MATERIALS AND METHODS. Immunoblots were incubated with monospecific polyclonal antibodies against C/EBP (A) or C/EBP (B) or preimmune serum as a control (unpublished data). (A) Anti-C/EBP. (B) Anti-C/EBP. Lanes  and  represent nuclear extracts from COS-1 cells transfected with C/EBP or C/EBP expression vectors and were used as standards for each protein. No bands were detected with preimmune serum with liver nuclear extracts or COS-1 nuclear proteins (unpublished data). Positions of molecular mass standards (kDa) are indicated on the left.

Identification of Specific C/EBP and C/EBP Isoforms That Bind to the APRE

To identify the C/EBP isoforms present in C1-C5, we isolated the complexes from EMSA gels and identified the proteins bound to the APRE by Southwestern blot and Western blot analyses. Previously, we showed by Southwestern blot analyses of proteins from 4-mo-old control liver nuclear extracts that the C1-C3 complexes consist of 42-kDa and 35-kDa isoforms, and that the C3 and C4 complexes formed with LPS-treated nuclear extracts consist mainly of a 20-kDa isoform with detectable amounts of 35-kDa and ~14.5-kDa proteins (An et al., 1996). Western blot analyses showed that the C1 and C2 complexes in control nuclear extracts consist of p42^C/EBP; the C3 complex consists of the p42^C/EBP and p35^C/EBP; the C3 and C4 complexes of LPS-treated nuclear extract consist mainly of p20^C/EBP (An et al., 1996). To identify the specific C/EBP isoforms bound to the C1-C5 complexes of the aged nuclear extracts, these complexes were isolated from EMSA gels and their proteins were analyzed by Southwestern and Western blot analyses. The Southwestern blot data in Figure 5A show the DNA-binding activity of C/EBP isoforms in aged control (lane 1) and LPS-treated (lane 2) nuclear extracts. The C1-C3 complexes showed a slight band at 42 kDa and a more intense band at 35 kDa; the major protein component of these bands is the 20-kDa isoform (Figure 5A, lane 3). The Western blot analyses show that only a very low level of p42^C/EBP can be detected by anti-C/EBP (just barely detectable), whereas p35^C/EBP and p20^C/EBP are detected by anti-C/EBP (Figure 5, B and C, lanes 3). These data show that in the aged nuclear extracts, the C1-C3 complexes are predominantly C/EBP isoforms. This is consistent with the EMSA and supershift analyses of the aged nuclear extracts which show a virtual loss of the C1-C2 complexes and persistence of the C3 complex.

View larger version (27K): [in this window] [in a new window]   Figure 5.   Identification of the C/EBP proteins that interact with APRE oligonucleotide to form C1, C2, C3, C4, and C5 complexes in livers of aged mice in response to LPS. The C1-C3, C4, and C5 complexes formed with control or LPS-treated (6 h) nuclear extracts from 28-mo-old C57BL/6 mice were resolved by preparative EMSA. The bands were excised from the gel, eluted, concentrated, and subjected to Southwestern blot and to ECL-Western immunoblot analyses as described in Ref. 3. (A) Southwestern analysis of liver nuclear proteins from 28-mo-old mice that form the C1-C3, C3, C4, and C5 DNA-protein complexes with the APRE oligonucleotide. Lane 1, control NE; lane 2, LPS-treated NE; lane 3, C1-C3 complexes formed with control NE; lane 4, C4 complex formed with control NE. Lanes 5 and 6, C3 and C4 complexes, respectively, formed with mouse liver NE after 6 h of LPS treatment. Lane 7, C5 complex 6 h after LPS treatment. (B and C) Western blot analysis using anti-C/EBP and anti-C/EBP and the same nuclear extracts and complexes as in A.

Southwestern blot analysis of the protein components of the C3, C4, and C5 complexes of aged LPS-treated nuclear extract show that a 20-kDa isoform has the highest level of binding activity (Figure 5A, lanes 4-7); the Western blot analyses of the C3, C4, and C5 complexes identify this protein as p20^C/EBP with some p14.5^C/EBP (Figure 5C, lanes 4-7). Furthermore, the data show that only anti-C/EBP detects the ~14.5-kDa isoform.

Identification of C/EBP and C/EBP Isoforms in Polysomes of Control and LPS-treated Livers by EMSA and Supershift Analyses

EMSA and supershift analyses of polysomal proteins from young and aged livers were done to demonstrate that polysomal nascent chains that contain the dimerization domains can be detected by gel-shift analysis, and that the molecular weights of proteins with binding activity are similar to those of the nuclear extracts. We argued that this would demonstrate the presence of nascent chains originating from multiple AUG sites, by alternative translational initiation, within the C/EBP and C/EBP mRNAs. Figure 6 shows EMSA and supershift analyses with polysomal proteins (Figure 6) from livers of young and aged mice with or without LPS. Figure 6A shows that the polysomal proteins from young livers interact with the oligonucleotide to form DNA-protein complex bands. The control bands (Figure 6A, lane 1) show a displacement by anti-C/EBP (Figure 6A, lane 2) and a low level interaction with anti-C/EBP (Figure 6A, lane 3); bands are also formed with proteins from LPS-treated polysomes (Figure 6A, lane 4) which are specifically displaced by anti-C/EBP (Figure 6A, lane 5) and anti-C/EBP (Figure 6A, lane 6). Furthermore, as with the LPS-treated nuclear extracts there is a reduction in the intensity of radioactive complex displaced by anti-C/EBP (Figure 6A, lanes 2 and 5; Figure 2A).

View larger version (122K): [in this window] [in a new window]   Figure 6.   EMSA and supershift analysis of mouse liver polysomal proteins that bind to the APRE oligonucleotide. Liver polysomes from young (A, 4-mo-old) and aged (B, 28-mo-old) control and LPS-treated mice were prepared as described in MATERIALS AND METHODS. The [32P]APRE oligonucleotide was prepared as described in MATERIALS AND METHODS. (A) Liver polysomal proteins from 4-mo-old mice; lanes 1-3, control; lane 2, anti-C/EBP; lane 3, anti-C/EBP; lanes 4-6, liver polysomal proteins from 4-mo-old LPS-treated mice; lane 4, no antibody; lane 5, anti-C/EBP; lane 6, anti-C/EBP. (B) Liver polysomal proteins from 28-mo-old mice; lanes 1-3, control; lane 2, anti-C/EBP; lane 3, anti-C/EBP; lanes 4-6, LPS treated; lane 4, no antibody; lane 5, anti-C/EBP; lane 6, anti-C/EBP; lane F, free probe.

The EMSA and supershift analyses of polysomal proteins from aged livers are shown in Figure 6B. DNA-protein complex formation occurs with polysomal proteins from both control and LPS-treated mice (Figure 6B, lanes 1 and 4). Supershift analyses of the polysomal proteins indicates that a C/EBP-DNA complex is shifted by anti-C/EBP in control (Figure 6B, lane 2) and LPS-treated polysomes (Figure 6B, lane 5). In addition, supershifting with anti-C/EBP detects polysome-associated C/EBP in controls (Figure 6B, lane 3) and a significant increase by LPS treatment (Figure 6B, lane 6). Thus, our data suggest that C/EBP peptides are detected on polysomes from LPS-treated aged livers, even though the level of C/EBP in the nuclear extract is significantly lower. These results suggest that the nuclear translocation and/or stability of C/EBP is altered in aged liver. On the other hand, the LPS-mediated increase in polysome-associated C/EBP corresponds with the increase in C/EBP nuclear extract pool levels seen in LPS-treated aged livers.

Southwestern Blot Analyses of Nuclear and Polysomal Proteins

In a previous article, we proposed that the C/EBP and C/EBP isoforms are products of differential translation of AUG initiation codons within a single mRNA and that the specific pattern of isoform synthesis in response to LPS is mediated via the mechanism of alternative translational initiation. To support our hypothesis, we conducted experiments to analyze the binding activity and molecular weights of nascent chains on C/EBP polysomes (Figure 7A; An et al., 1996). We argued that the presence of C/EBP isoform nascent peptides on the polysomes would support our hypothesis that they are formed by alternative translational initiation. These Southwestern blot analyses showed that proteins isolated from purified polysomes exhibit a similar pattern of DNA-binding activity as that observed with liver nuclear extracts, i.e., the polysomal proteins from control livers exhibited binding activity at 42 kDa, 35 kDa, 30 kDa, 20 kDa, and ~14.5 kDa (Figure 7A; An et al., 1996). Furthermore, in LPS-treated livers the binding activity of the 42-kDa, 35-kDa, and 30-kDa polysomal proteins is decreased, whereas that of the 20-kDa proteins increased. These data indicated that the nascent chains, i.e., 42 kDa, 35 kDa, 30 kDa, and 20 kDa, whose molecular masses correspond to AUG sites in the mRNAs are the products of differential initiation of translation. To determine whether a similar mechanism occurs in aged livers, we conducted Southwestern blot analysis of the polysomal proteins of aged control and LPS-treated livers. The data in Figure 7B, lanes 1 and 2, show the expected patterns with nuclear extracts from aged control and LPS-treated livers. Similar analysis of the polysomal proteins also detected the expected isoforms, i.e., 42 kDa, 35 kDa, 30 kDa, and 20 kDa (Figure 7B, lanes 3-5). However, in contrast to the data with nuclear extracts, which show that the 42-kDa isoform is not detectable after LPS treatment, we have detected this protein in polysomes of aged livers at 3 and 6 h after LPS treatment. These data suggest that in the aged liver, the translation of the 42-kDa C/EBP is not down-regulated by LPS. The 20-kDa isoforms, on the other hand, show a pattern of synthesis similar to that seen for polysomal proteins from young livers, i.e., the level of the 20-kDa isoform is low in control and increases in LPS-treated polysomes. These data indicate that although the synthesis of p42^C/EBP persists in aged liver, its decreased pool level and DNA-binding activity in aged nuclear extracts suggest that its translocation to the nucleus may be attenuated or that its stability may be altered.

View larger version (34K): [in this window] [in a new window]   Figure 7.   Southwestern blot analysis of the mouse liver polysomal proteins from young (A, 4 mo) and aged (B, 28 mo) old livers. (A) Lane 1, control NE; lane 2, LPS-treated NE; lane 3, control polysomal proteins; lane 4, polysomal proteins 3 h after LPS treatment. (B) Lane 1, control NE; lane 2, LPS-treated NE; lane 3, control polysomal proteins; lane 4, polysomal proteins 3 h after LPS treatment; lane 5, polysomal proteins 6 h after LPS treatment.

Analysis of Isoform Production by C/EBP Expression Vectors Transfected into COS-1 Cells

We have hypothesized that the qualitative and quantitative changes in C/EBP and C/EBP isoform pools in response to LPS may occur through the alternative translational initiation of specific AUG start sites. The differential translation of C/EBP mRNAs is supported by reports that the p30^C/EBP, p20^C/EBP, and p20^C/EBP are products of the alternative translational initiation of the C/EBP and C/EBP mRNAs (Descombes et al., 1990; Descombes and Schibler, 1991; Ossipow, et al., 1993; Lin and Lane, 1994; An et al., 1996). Furthermore, others have presented evidence that the formation of low molecular weight C/EBP and C/EBP isoforms is not due to proteolytic cleavage of the high molecular weight isoforms (Calkhoven et al., 1994; Sears and Sealy, 1994). In these studies we present evidence to support our hypothesis that C/EBP isoform synthesis may occur by alternative translational initiation. To demonstrate this, we transfected two C/EBP expression vectors into COS-1 cells and analyzed for their ability to produce C/EBP isoforms. Each of the expression vectors contained Flag sequences at their C-terminal ends to demonstrate by immunoblot that the origin of their isoforms is from the transfected C/EBP expression vectors. The data in Figure 8 show the C/EBP isoforms synthesized by the wild-type expression vector pCMV-C/EBP (lanes 3, 7, and 11) and an expression vector (pCMV-C/EBPMT20) in which the 20-kDa AUG start site was mutated to TTG (lanes 4, 8, and 12). Using anti-Flag antibody, we detected the 35-kDa and 20-kDa isoforms synthesized by wild-type pCMV-C/EBP (Figure 8, lane 3). The data in lane 7 (Figure 8) show that with the pCMV-C/EBPMT20 the synthesis of 35-kDa isoform persists whereas the formation of the 20-kDa isoform is completely abolished. Furthermore, the data show that the ~8.5-kDa isoform appears, suggesting that silencing of the 20-kDa start site may facilitate initiation at a downstream AUG site located within the DNA-binding domain (Figure 8). Similar results were obtained using antibody to the C-terminal sequences of C/EBP, i.e., the wild-type mRNA translates 35-kDa and 20-kDa isoforms (Figure 8, lane 7) and the mutation of the 20-kDa start site eliminates formation of this isoform, whereas the 35-kDa isoform is not affected (Figure 8, lane 8).

View larger version (47K): [in this window] [in a new window]   Figure 8.   The transcription and translation of wild-type and mutant pCMV-C/EBP expression vectors in COS-1 cells. Western blot analysis of the C/EBP isoforms synthesized in COS-1 cells transfected with wild-type pCMV-C/EBP and pCMV-C/EBP mutated at the 20-kDa AUG start site. The AUG was mutated to TTG. The expression vectors contain a Flag tag at their C-terminal ends. Western blot analyses were performed on COS-1 nuclear extracts using anti-Flag (lanes 1-4), anti-C-terminal C/EBP-antibodies (lanes 5-8); and anti-N-terminal C/EBP antibody (lanes 9-13). Southwestern blot analysis was performed on the COS-1 nuclear extracts used for the immunoblot analyses (lanes 13-16). Lanes 1, 5, 9, and 13, control COS-1 nuclear extract; lanes 2, 6, 10, and 14, nuclear extracts of COS-1 cells transfected with expression vector lacking C/EBP sequences (pCMV-B); lanes 3, 7, 11, and 15, nuclear extracts of COS-1 cells transfected with wild-type pCMV-C/EBP; lanes 4, 8, 12, and 16, nuclear extracts from COS-1 cells transfected with pCMV-C/EBP mutated at 20-kDa AUG start site. Maps of the C/EBP expression vectors used in these experiments are shown below the autoradiogram.

Although our analyses of polysomal nascent chains (Figure 7) and the studies described above indicate that C/EBP isoforms may be formed by alternative translational initiation, our experiments do not rule out the possible occurrence of a specific proteolytic cleavage of the nascent chains of the high molecular weight isoforms or specific cleavage of the mature polypeptide. Since p20^C/EBP is a major isoform synthesized in response to LPS (in both young and aged livers and in the COS-1 cells), to address this, we prepared antibody to the N-terminal region, downstream of the p35^C/EBP AUG start site (Figure 8, lanes 9-12). If proteolytic cleavage occurs, this antibody would detect a 16-kDa N-terminal fragment that has no binding activity. The data in Figure 8 show that only the 35-kDa C/EBP isoform is detected in COS-1 cells transfected with both wild-type (lane 11) and mutant (lane 12) pCMV-C/EBP expression vectors.

Southwestern blot analyses of the products of these C/EBP expression vectors show that the high molecular weight isoforms have DNA-binding activity, whereas the ~8.5-kDa isoform does not. There is an AUG start site that could account for this isoform that is located within the DNA-binding domain that would explain the lack of binding activity by this protein (Figure 8). Further studies are in progress to demonstrate initiation at this site.

Role of p20^C/EBP in the Activation of AGP Gene Expression via Its Interaction with the APRE

Our studies suggest that the LPS-mediated activation of the AGP gene in aged and young mouse livers may be mediated by the interaction of p20^C/EBP with the C/EBP binding site (APRE) of the AGP promoter. Other studies have indicated that because of the truncated transcription activation domain, p20^C/EBP could not serve as an efficient transactivator. It has been shown, for example, that p20^C/EBP (LIP) has repressor activity with the albumin D promoter binding site (Descombes and Schibler, 1991). To determine whether p20^C/EBP can function as a transactivator, we cotransfected the pMSV-C/EBP^20-kDa and pAPRE-CAT expression vectors into COS-1 cells. The data in Figure 9 show that increasing concentrations of transfected pMSV-C/EBP^20kDa can drive activation of pAPRE-CAT expression, suggesting that the 20-kDa C/EBP isoform can function as a transactivator. Since APRE is a composite C/EBP-glucocorticoid receptor binding site, the role of other factors such as glucocorticoid receptor, in maximal activation of the AGP gene via the APRE, remains to be shown.

View larger version (46K): [in this window] [in a new window]   Figure 9.   The transactivation of pAPRE-CAT expression by p20C/EBP. COS-1 cells were cotransfected with pMSV-C/EBP20-kDa and pAPRE-CAT expression vectors to determine whether expression of the 20-kDa C/EBP isoform could activate expression of pAPRE-CAT. A typical autoradiogram depicting chloramphenicol acetyl transferase activity in response to increasing expression of 20-kDa C/EBP is shown in the upper panel. Lane 1, the pSV2-CAT expression vector minus the APRE sequences; lane 2, pAPRE-CAT; lanes 3-5, pAPRE-CAT + 1, 2, and 3 µg of pMSV without the 20-kDa C/EBP sequences, respectively; lanes 6-8, pAPRE-CAT + 1, 2, and 3 µg of pMSV-C/EBP20-kDa, respectively. A presentation of the data from three separate experiments is shown in the lower panel.

	DISCUSSION

Top Abstract Introduction Materials & Methods Results Discussion References

In previous studies, we demonstrated the presence of multiple forms of C/EBP and C/EBP proteins in the young mouse liver and dramatic changes in their DNA-binding activities and protein pool levels in response to LPS treatment (An et al., 1996). These studies showed that the level of p42^C/EBP is reduced and the levels of p20^C/EBP and p20^C/EBP are stimulated by LPS treatment (see maps in Figure 10). We concluded that the formation of C/EBP and C/EBP isoforms occurs by alternative translational initiation at multiple AUG sites within the single C/EBP and C/EBP mRNAs, and that this differential initiation of translation occurs via the leaky ribosomal scanning mechanism (Kozak, 1989, 1992, 1995). In the present studies, we have shown that the DNA-binding activities and pool levels of C/EBP and C/EBP isoforms are significantly altered in the aged liver. This change is particularly significant in control aged livers, where the p42^C/EBP nuclear pool level is decreased and the p20^C/EBP pool level is increased. This age-associated pattern of binding activity is typical of the inflammatory response in livers of young LPS-treated mice. In particular, the EMSA and super gel-shift pattern of control liver nuclear extract from aged animals show an increase in C/EBP-DNA-protein complexes (C3 and C4). Our data further suggest that aging results in an altered regulation of C/EBP and C/EBP isoform synthesis and/or changes in the turnover of these proteins or in their ability to translocate to the nucleus. We conclude that the normal aged liver exhibits characteristics typical of a young liver during an inflammatory response.

View larger version (24K): [in this window] [in a new window]   Figure 10.   A map of the C/EBP (A) and C/EBP (B) mRNAs. The positions of the AUG initiation sites within each mRNA and the Kozak sequences relative to the A (+1) in each AUG are indicated. The numbers indicate the position of the As in each initiation codon. The molecular masses of isoforms that can be formed by initiation at the designated AUG sites are depicted in parentheses behind each Kozak sequence. The MPGEL is the amino acid sequence of the C/EBP sORF and MPPAAARLL is the amino acid sequence of the C/EBP sORF.

Another interesting age-associated change revealed by the EMSA time course and corresponding protein pool levels is that the recovery from the acute phase response, characterized by the return of the p42^C/EBP-DNA complexes (C1 and C2) to normal levels, is significantly delayed. In the young animal the formation of the p20^C/EBP-DNA complexes (C3 and C4) persists from 3 to 12 h after LPS whereupon the 42-kDa C/EBP-DNA complexes (C1 and C2) reappear, indicating recovery from the inflammatory response. The time-course analyses clearly show that in young and 12-mo-old livers, recovery from the acute phase response nears completion between 12 and 24 h after LPS treatment. However, in the aged animals, the EMSA, Southwestern blot, and Western blot analyses show that there is no recovery from this inflammatory response even 48 h after LPS. Our recent preliminary studies indicate that the recovery occurs 72-96 h after LPS (our unpublished results). Thus, the time required for recovery from an inflammatory response is significantly prolonged in the aged animala further indication that the physiological stress response processes are altered in normal aged tissue. We speculate that this delayed recovery may be due to a combination of continued stimulation of p20^C/EBP synthesis and a failure to reinitiate p42^C/EBP synthesis. However, the analysis of polysomal peptides indicates that the p42^C/EBP is synthesized, which suggests that there may be a failure of nuclear translocation or a destabilization of C/EBP. We propose, therefore, that the control, unchallenged, aged animal exhibits characteristics of chronic inflammatory stress as indicated by the qualitative and quantitative pool levels of C/EBP isoforms and their DNA-binding activities, which mimic the acute phase response, and that upon challenges such as an induced acute phase response, the recovery is significantly delayed.

Immunological and DNA-binding activity analyses have demonstrated the in vivo and in vitro formation of multiple C/EBP and C/EBP DNA-binding proteins with varying molecular weights which also have various transcriptional activities (Descombes and Schibler, 1991; Lin et al., 1993; Ossipow et al., 1993; Calkhoven et al., 1994; Lin and Lane, 1994; Sears and Sealy, 1994; Clarkson et al., 1995; An et al., 1996). It has been proposed that synthesis of these proteins occurs by alternative translational initiation of multiple AUG sites within the single C/EBP and C/EBP mRNAs. In our studies we showed that LPS specifically mediates the reduction of p42^C/EBP synthesis and induction of synthesis of p20^C/EBP and p20^C/EBP isoforms, and we propose that this may occur by differential initiation of translation at specific AUG sites. Our hypothesis is supported by the observation that the DNA-binding activity of polysomal nascent peptide chains corresponds closely with the patterns observed with nuclear extract (An et al., 1996). Since C/EBP DNA-binding activity is dependent on their ability to dimerize, analyses of the polysomal nascent chains only detected those that contain the leucine zipper sequences at the carboxyl terminus. The ability to detect the C/EBP homodimers in polysomal protein extracts by binding activity supports our hypothesis that these isoforms are produced by an alternative translational initiation mechanism. Further support for the alternative translational initiation of the C/EBP mRNA is provided by our observations that C/EBP isoforms (35 kDa and 20 kDa) are synthesized in COS-1 cells transfected with wild-type pCMV-C/EBP expression vector. Mutation of the 20-kDa AUG start site to TTG did not affect the synthesis of the 35-kDa isoform while formation of the 20-kDa isoform was abolished. Our data suggest that production of the 20-kDa C/EBP isoform occurs via alternative translational initiation rather than via the proteolytic cleavage of the high molecular mass 35-kDa isoform.

Our immunological and DNA-binding activity data have demonstrated significant changes in the levels of high and low molecular weight C/EBP and C/EBP isoforms. Analysis of the nascent chains from polysomes of aged livers indicated that the p42^C/EBP, which is down-regulated on young polysomes, is not affected, or may even be up-regulated by LPS in aged polysomes. Since the nuclear pool levels of p42^C/EBP are reduced in control aged liver and further reduced by LPS treatment, our data suggest that two major regulatory changes may occur in the aged liver. The first involves an age-associated failure to down-regulate C/EBP nascent chains on polysomes, which we observed to occur in young livers (An et al., 1996). This suggests an age-associated attenuation of the alternative translational initiation mechanism that conditions the scanning ribosome to bypass the 42-kDa AUG initiation site in response to LPS. At the same time, since the 20-kDa C/EBP isoform is stimulated, this indicates that the alternative translational initiation at this AUG site does occur. However, our results do not rule out the possibility of a specific processing or proteolytic cleavage of the high molecular weight nascent chains, which does not occur in young polysomes. The report that m-calpain specifically cleaves several transcription factors, leaving the binding and dimerization domains intact, suggests that some low molecular weight isoform may be a product of such cleavage (Watt and Molloy, 1993). Whether m-calpain can cleave polysomal nascent chains is presently being investigated. Sequence analysis of the isoforms, which is also in progress, would demonstrate whether the N-terminal amino acid residue is methionine.

The second age-associated regulation involves a possible attenuation of the nuclear translocation of p42^C/EBP. This is suggested by the observation that p42^C/EBP synthesis persists in polysomes from aged livers, whereas the nuclear pool level decreases, and by the fact that the p20^C/EBP isoforms in these polysomes and their nuclear pool levels increase. A consequence of the decreased efficiency of nuclear translocation of p42^C/EBP and continued translocation of C/EBP could be a factor in the failure of the liver to recover from the inflammatory response. The studies of Yin et al. (1996) have shown that tumor necrosis factor-, a mediator of the inflammatory response, initiates a rapid posttranscriptional activation and nuclear translocation of C/EBP and C/EBP in hepatocytes. They also confirmed our observation that the C/EBP is decreased in this inflammatory response. These observations support our proposal that nuclear translocation of C/EBP may be affected by aging. This reduced level of p42^C/EBP in the nucleus may favor C/EBP binding and may in part cause the prolonged stress response indicated by our EMSA data. We propose that the inability of p42^C/EBP to translocate to the nucleus favors the prolonged presence of the p20^C/EBP-APRE complex, which may be a factor in the prolonged inflammatory response in aged liver.

Alternative translational initiation at the p20^C/EBP- and p20^C/EBP-specific AUGs occurs in both young and aged livers in response to LPS. However, as we argued above, our experiments do not completely rule out the occurrence of a very specific proteolytic cleavage of the nascent chain of a high molecular weight isoform. To address this possibility, we prepared an antibody to the NH-terminal region downstream of the p35^C/EBP start site which would detect the product of proteolytic cleavage at either the 30-kDa or 20-kDa start sites. If proteolysis occurs, this antibody would detect a 6-kDa and/or a 16-kDa NH-terminal fragment that would not have DNA-binding activity. Our results detect only the 35-kDa C/EBP isoform in the COS-1 cells transfected with the expression vector mutated at the 20-kDa start site. These results suggest that p30^C/EBP and p20^C/EBP are not products of proteolytic cleavage at the second and third AUG start sites. Similar analyses with antibodies to the NH-terminal region of C/EBP are in progress.

Our experiments indicate that the LPS-induced increase in the newly synthesized polysome-associated p20^C/EBP in aged (these studies) as well as young (An et al., 1996) livers is far less than the increase in nuclear pool level of this protein. These data suggest that in addition to the translational increase there is a stabilization of p20^C/EBP which results in its increased nuclear pool level. We speculate that posttranslational modification, such as phosphorylation or protein-protein interactions associated with nuclear translocation and the formation of DNA-protein complexes in the nucleus, may be important factors in the stabilization of this protein and its elevated pool level in the nucleus. Similarly, the decreased pool level of p42^C/EBP in aged nuclear extract may be due to age-associated changes in posttranslational modifications, which may be a factor in destabilization or the basis for the isoform's decreased translocation to the nucleus. Studies from other laboratories have shown that phosphorylation of C/EBP and C/EBP occurs at specific sites and that some of these modifications affect the binding activity of these proteins (Metz and Ziff, 1991; Mahoney et al., 1992; Wegner et al., 1992; Trautwein et al., 1993). We propose that aging may affect the interactions of signaling pathways which may modify C/EBP and alter its stabilization and translocation. It is interesting that a model of the structure of the C/EBP molecule and its mechanism of activation is based on its ability to adopt a tightly folded conformation that masks the DNA-binding and activation domains, and that phosphorylation causes the protein to unfold and unmask these domains (Williams et al., 1995). Such a structure may also serve to stabilize this protein for nuclear translocation and may be the basis for the st