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MBC Figures for Molecular Biology of the Cell: Volume 0, Issue 2002 -- Page E02-05-0294

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Supplemental Figures (PDF)

Figure S1. Immunoblot of WASP/Scar1 derivative-coated beads probed with anti-FLAG antibody showing comparable concentrations of protein bound to the beads.

• Figure S1

Figure S2. WIP binds to recombinant WASP. Beads coated with WASP or WASP-GPWCA were incubated in human platelet extract, and bound proteins were eluted by boiling in SDS-PAGE sample buffer and separated by SDS-PAGE. The presence of WIP was detected by Western blotting using anti-WIP antibody (top panel). The presence of WASP/WASP-GPWCA was detected by Western blotting with anti-FLAG antibody (bottom panel). Lanes represent, from left to right: platelet extract, proteins bound to WASP-GPWCA-coated beads, proteins bound to WASP-coated beads.

• Figure S2

Figure S3. Regulation of WASP-BGPWCA and WASP-GPWCA by Cdc42 and PIP₂. (A,B) Graphs of fluorescence intensity versus time after initiating actin polymerization in the pyrene-actin polymerization assay. (A) 2 muM actin in the presence of 10 nM Arp2/3 complex, 30 nM WASP-BGPWCA, and 300 nM Cdc42-V12 (GTPgammaS), and/or 30 muM PIP₂. (B) 30 nM WASP-GPWCA, and 300 nM Cdc42-V12 (GTPgammaS), and/or 30 muM PIP₂.

• Figure S3

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