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MBC Video for Molecular Biology of the Cell: Volume 0, Issue 2002 -- Page E02-06-0330

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• LegrosMv1.mov

Punctate mitochondria of cccp-treated cells are mobile, reach and maintain close proximity, but do not fuse. Shown is a selected region of a cell treated with 10 µM cccp for 4h. The dynamics of mtRFP-labeled mitochondria was followed for 53 min. The elapsed time is indicated in mm:ss. Acquisition interval, 30 s; exposure time, 0.2 s; and movie speed, 6 frames/s (3 min/s).

• LegrosMv2.mov

After removal of cccp, fusion is restored and mitochondria reacquire a tubular morphology. Cells were treated with 10 µm cccp for 4h. Shown is a selected region 1h after cccp-removal (time 00:00). The dynamics of mtRFP-labeled mitochondria were followed for 58 min. The elapsed time is indicated in mm:ss. Acquisition interval, 30 s; exposure time, 0.2 s; movie speed, 6 frames/s (3 min/s).

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This Article Abstract Full Text Services Alert me to new issues of the journal