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MBC Video for Molecular Biology of the Cell: Volume 0, Issue 2002 -- Page E02-06-0346

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Supplemental Figure

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  Endogenous EMR-1 is mislocalized when the Ran GTPase cycle is perturbed. Embryos from GFP::histone H2B worms grown on either control bacteria (A), or bacteria expressing dsRNA corresponding to Ran (B, C), RanGAP (D), or RanBP2 (E) were fixed and analysed with polyclonal antibodies against EMR-1. Single confocal sections are shown demonstrating that in wild type embryos EMR-1 is present at the nuclear periphery in interphase and until late in mitosis. In contrast, in RNAi embryos the chromatin either is not associated with EMR-1 or is associated with large aggregates of EMR-1. Top row shows GFP::histone H2B in green, middle row shows anti-EMR-1 staining in red; bottom row shows an overlay of the two signals. Bar, 10 µm.

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