3D projection showing the distribution of EB1-GFP at the tips of growing microtubules in a late prophase cell (shown as a 2D projection in Figure 2B). The projection shows the high density of microtubule ends growing in close association with the nuclear envelope.
Digital movie of the "movement" of EB1-GFP comets in a late prophase cell. In this cell the two centrosomes were located near the top of the nucleus and many microtubules grow along the surface (prominent on the left side of the image). Image stacks were collected by wide field microscopy.
A second example of EB1-GFP "movement" in a late prophase cells. Microtubules originating from the bottom centrosome can be seen growing along the left side of the nuclear envelope. Image stacks were collected by wide field microscopy.