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This item has the following additional materials available:
Mitosis.mov (6.46MB) Figure 8. Live cell observations of entry of SF2/ASF into daughter cell nuclei. HeLa cells were transiently transfected with YFP-SF2/ASF and live-cell observations were initiated 2 days post-transfection. Metaphase cells exhibit 1-2 MIGs near the metaphase plate (a, arrow). As the cell enters anaphase, MIGs become more abundant (b and c). SF2/ASF begins to enter daughter nuclei at telophase (d). Nuclear entry of YFP-SF2/ASF is nearly complete in approximately 20 min (h). Time is indicated in min from the initiation of imaging the metaphase cell.
Prepared by: the MBC Journal Production Manager
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