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MBC Video for Molecular Biology of the Cell: Volume 0, Issue 2003 -- Page E02-10-0677

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• movie_1.mov Figure 3. Movement of XBX-1::YFP along the cilia axoneme in phasmid neurons of a wild type hermaphrodite. Using time-lapse fluorescence image analysis, XBX-1::YFP movement was observed in both (A) anterograde and (B) retrograde directions similar to that seen for other IFT proteins (Signor et al., 1999a; Haycraft et al., 2001; Qin et al., 2001). The transition zone is marked (*). The arrowhead indicates the initial position of the particle at time zero (t = 0). The arrows indicate the same particle at subsequent times (t = 0.5 seconds, and t = 1 seconds). The distal tips of the phasmid cilia are directed toward the left. Three sequential frames from Movie 1 are shown for each.

• movie_2.mov Figure 7. Movement of XBX-1::YFP along the cilia axonemes on one pair of phasmid neurons of a che-11 complex A mutant. Using time-lapse fluorescence image analysis, XBX-1::YFP movement was observed in the (A) anterograde direction along the stunted cilia axoneme of the che-11 mutants. In contrast to other IFT proteins, XBX-1::YFP transport was also detected in the (B) retrograde direction. This continued retrograde trafficking of XBX-1 in che-11 mutants is consistent with the failure of XBX-1 to accumulate in the cilia axoneme. Images were captured at a rate of two frames per second. The distal tips of the phasmid cilia are directed toward the left. The transition zone is marked (*). The arrowhead indicates the initial IFT particle at time zero (t = 0). The arrows indicate the same particle at the subsequent times indicated (t = 0.5 seconds, and t = 1 seconds). Three frames representative of the movement seen in Movie 2 are shown for each direction.

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