MBC Videos

This article contains the following videos and supplemental material:

The videos available in the online version correspond to the video stills shown in figure 8B. PC12 cells expressing TI-VAMP-GFP were incubated with either L1-coated beads (Video 1) or N-Cadherin-coated beads (Video 2) and the GFP signal of cells associated with beads was acquired every 10 s for 300 ms per acquisition. 77 (Video 1) and 83 (Video 2) frames are shown at 1 frame per 1/6 s.
• Video 1 (1.88MB)
• Video 2 (1.23MB)

• Supplemental Figures (PDF)
• Supplemental Fig 1) L1 stimulates axonal outgrowth in hippocampal neurons. Poly-L-Lysine-coated coverslips were incubated with anti-human Fc antibodies followed by incubation with (L1) or without (poly-Lysine) L1-Fc fragment. Hippocampal neurons seeded at low density were kept in culture for 24 hours and analyzed by immunofluorescence with pAb to L1.
• Supplemental Fig 2) Quantification of equilibrium gradient fractionation from PC12 cells. The signals corresponding to TI-VAMP, L1 and Na/K-ATPase immunoreactivity were quantified by densitometry; the peak signal was defined as 1.
• Supplemental Fig 3) Accumulation of TI-VAMP in growth cones contacting L1 beads is a robust phenomenon. Hippocampal neurons grown for three day in vitro were incubated with L1 coated beads and processed for confocal microscopy analysis with mAb 158 and pAb to L1. Images were taken based on L1 fluorescence thus blind for TI-VAMP immunoreactivity. Accumulation of TI-VAMP reactivity at sites of bead contact were scored as positive (+) and no accumulation as negative (-). (Bead diameter represents 4µm).

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