MBC Additional Material

This article contains the following material:

Videos

• Movie1-Fig2C.mov (8.06 MB) Gradient effect of antibody inhibition Anti-KLP3A and rhodamine tubulin injected GFP tubulin embryo displays gradient effects on spindle pole separation. anti-KLP3A was injected into region A in cell cycle 10.
• Movie2-Fig3.mov (5.47 MB) IgG injected GFP-CID embryo Control IgG and rhodamine tubulin injected GFP-CID embryo. GFP-CID, a kinetochore marker, and tubulin are shown in green and red, respectively.
• Movie3-Fig3.mov (6.55 MB) Anti-KLP3A injected GFP-CID embryo Anti-KLP3A and rhodamine tubulin injected GFP-CID embryo. As kinetochore MTs disassemble, spindles do not elongate properly with splayed morphology and they do not contain robust interpolar MT bundles (arrows) and telophase midbodies which are shown in control.
• Movie4-Fig4.mov (5.62 MB) IgG injected GFP-HIS embryo Control IgG and rhodamine tubulin injected GFP-HIS embryo
• Movie5-Fig4.mov (6.63 MB) Anti-KLP3A injected GFP-HIS embryo Anti-KLP3A and rhodamine tubulin injected GFP-His embryo. Antibody was injected into bottom of the embryo. KLP3A inhibition does not disrupt initial bipolar spindle assembly but does result in shorter spindles which do not elongate properly and daughter nuclear collapse in telophase.
• Movie6-Fig5C.mov (4.44 MB) Anti-KLP3A injected Ncd null Anti-KLP3A injected Ncd null embryo.
• Movie7-SFig3.mov (8.81 MB)
KLP3A stalk injection Dominant negative KLP3A stalk protein and rhodamine tubulin injected GFP-HIS embryo

Supplemental Data (PDF)

• Supplemental Figures 1-3
• Supplemental Tables
• Supplemental Figure 1
• Supplemental Figure 2
• Supplemental Figure 3

Prepared by: Journals Production Manager