MBC Videos

This article contains the following supporting material:

• Movie 1 (8.08 MB) Time-lapse epifluorescence microscopy of confluent PK18-5 cells depicting continuous inward flow of KF fluorescence from the entire cell circumference. Recording intervals: 30 s.
• Movie 2 Time-lapse epifluorescence microscopy of peripheral free edge of a PK18-5 cell showing centripetal flow of HK18-YFP fluorescence originating in a submembraneous compartment and resulting in integration into the peripheral filament network. Recording intervals: 30 s.
• Movie 3 Time-lapse confocal laser scanning microscopy of a peripheral free region of a human primary keratinocyte depicting the inward-directed movement of HK14-YFP fluorescence which originates in a submembraneous compartment and results in integration of rod-like structures into the peripheral filament network. Recording intervals: 60 s.
• Movie 4 (3.08 MB) Time-lapse confocal laser scanning microscopy of a small peripheral section of a PK18-5 cell (projection of six focal planes) depicting the formation of KF precursors below the plasma membrane (position demarcated by line), their subsequent enlargement and integration into the filament network. Pictures were taken every 30 s.
• Movie 5 (4.11 MB) Time-lapse epifluorescence microscopy of a peripheral region of a SW13 cell producing fluorescent keratins HK8-CFP and HK18-YFP highlighting details of KF formation beneath the plasma membrane (top). Recording of EYFP-elicited fluorescence was every 30 s.
• Movie 6 (2.08 MB) Time-lapse fluorescence imaging of a FRAP experiment, in which a section of a PK18-5 cell producing HK18-YFP was bleached, demonstrating that filamentous fluorescence recovery occurs preferentially in the cell periphery. Recording intervals, 2 min.
• Movie 7 (9.43 MB) Time-lapse fluorescence image series depicting the distribution of HK13-EGFP in AK13-1 cells after a short treatment with orthovanadate (10 mM, 4 min). Recording intervals, 15 s.
• Movie 8 (7.35 MB) Time-lapse imaging of HK13-EGFP fluorescence (inverse presentation) in a peripheral region of an AK13-1 cell in the continued presence of orthovanadate (10 mM). Images were recorded every 30 s.
• Movie 9 Time-lapse fluorescence imaging of a peripheral part of a desmosome-free A-431 cells stably synthesizing a dominant-negative connexin-desmoglein chimera and producing HK13-EGFP after cDNA-transfection. Recording intervals, 30 s.

Prepared by: Journals Production Manager