Supplemental Material

This article contains the following supporting material:

• SuppFig1.pdf -
  
• SuppFig2.pdf -
  
• Supplemental Table.pdf - PCR primers used for fluorescent fusion proteins or gene disruption in this study.
  
  
• SuppMov1.mov - Tem1p-GFP in living cells (KBY 9201). Upper left panel – DIC image; upper right panel – epi-fluorescent image of Tem1p-GFP; lower left panel – overlay of DIC image (green) and Tem1p-GFP (red); lower right panel – timestamp in minutes:seconds.
  
  
• SuppMov2.mov - Reconstruction of representative cell expressing Lte1-3xGFP (PY 3548). Note the restriction of Lte1p-3xGFP to the bud cortex. Bar, 2 μm.
  
  
• SuppMov3.mov (2.43 MB) - Bub2p-GFP in living cells during spindle elongation (KBY 9221).
  
  
• SuppMov4.mov - Δkar9 cell expressing Tem1p-GFP demonstrates the changes in fluorescent intensity at the SPB’s over time as the mitotic spindle positions prior to anaphase onset (KBY 9205).
  

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