QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Abstract Full Text Services Alert me to new issues of the journal

Molecular Biology of the Cell: E03-10-0738

This article contains the following additional material:

Supplemental Tables

• Supplemental Tables PDF

Supplemental Table 1 – Complete data set of all genes considered for this study. The first column is the gene name. The second column is the accession number of the mRNA sequence used to measure the expression of the gene and to predict the promoter for the ChIP and luciferase experiments. The third column is the gene symbol. The fourth column is the HSF1 ChIP fold-enrichment measurement, and the last column is the fold-induced expression measurement as measured by real-time RT-PCR.

Supplemental Table 2 – Complete data set for the 176 genes assayed for both induced expression and HSF1 binding. The first column is the name of the category. The second column is the gene name. The third column is the accession number of the gene. The fourth column is the ChIP-enrichment measurement. The fifth column is the fold-induced expression measurement, and the last column is the highest scoring HSE in the promoter region of the gene as determined by the HSE PSSM evaluation.

Supplemental Table 3 – Heat shock genes arranged in an head-to-head orientation in the genome. This table describes the heat shock genes that use a potential bidirectional promoter. Included are the gene names, the induced expression measurements of each gene, the estimated distance between transcription start sites and the chromosome on which the pair is located.

Supplemental Figures

• Supplemental Figures PDF

Supplemental Figure 6 – Distribution of fold-induced expression measurements. This figure shows the distribution of all the relative expression measurements made with real-time RT-PCR in the human K562 cell line.

Supplemental Figure 7 – Distribution of HSF1 ChIP-enrichment measurements. This figure show the distribution of all the ChIP-enrichment measurements made with real-time RT-PCR in the human K562 cell line.

Supplemental Figure 8 – Distribution of HSE occurrence scores. This figure show the distribution of all the occurrence scores that were calculated for 2 kb human promoter sequences that were tested for binding of HSF1 by ChIP.

Supplemental Figure 9 – Expanded version of the clustered mouse microarray experiments shown in Figure 4.

Supplemental Figure 10 – Same as the cluster analysis in Figure 4, except that the data from each cell line were transformed by the zero time points. The expression changes in both cell types are shown for A) homologs of a subset of human genes that were HSF1-bound and induced, B) homologs that were bound by HSF1 but not heat induced, and C) homologs that were induced but not bound by HSF1 in the human system. The mouse microarray data was also analyzed independent of the human experiments, and these results show D) genes induced by heat in wild type but not HSF1-/- fibroblasts, E) genes induced in HSF1-/- fibroblasts, and F) genes induced similarly in wild type and HSF1 -/- fibroblasts. Panel G) shows the average expression profile for spots containing repetitive elements according to RepeatMasker and the standard error of each time point for each cell type.

Supplemental Figure 11 – Promoter activities of 48 random human promoters before and after heat shock. Values on the y-axis are the log (base 2) ratios of promoter activity before and after heat shock.

Prepared by: the Journals Production Manager

This Article Abstract Full Text Services Alert me to new issues of the journal