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Molecular Biology of the Cell: E03-10-0740

This article contains the following additional material:

Supplemental Figures (PDF)

  • Supp Figure 1 Mutants with severely degraded telomeres showed immediate slow growth followed by recovery.

  • Supp Figure 2 Chromosome segregation assay and the budding and FACS profile for template mutants. (A) The GFP-LacI is expressed from a Copper-inducible promoter and binds to a tandem array of 256 Lac operator sequences positioned either 12Kb from the centromere (cen) or about 100 Kb from the telomere (tel) of chromosome IV. (B) Bud profile for first cell cycle after release from α-factor. WT and all template mutants, marked at either the centromere or the telomere of chromosome IV was similar. Budded population includes small-budded, large-budded and rebudded cells. (C) FACS profile for each 20-minute timepoint for WT and cen-marked tlc1 (D), (E) and (SS).

  • Supp Figure 3 DNA-damage checkpoint proteins, Ddc1p and Ddc2p form foci in cells with tlc1 mutations.

    (A) Asynchronous log phase cells were analyzed, immediately after replacement of TLC1 with tlc(D), (E) or (SS) (P=0); after the first passage with tlc1 mutations (P=1); or after six passages (P=6). (B) representative images of Ddc1-GFP and Ddc2-GFP in WT and tlc1 mutants at t=6.

  • Supp Figure 4 The DNA Damage Checkpoint Dependence of cdc13-1 at 23˚.

    Wildtype and cdc13-1 strains were combined with deletions of DDC1, DDC2 or both, and chromosome dynamics were monitored as in Figure 4 for the 80, 100 and 120 minute timepoints. The chromosome segregation defect seen in cdc13-1 at 23˚ is completely relieved by deletion of DDC1 or DDC2.

  • Supp Figure 5 Disruption of the spindle assembly checkpoint did not relieve the chromosome missegregation phenotype seen in tlc1 mutants. Template mutations (D), (E) and (SS) were combined with Δmad2 and chromosome segregation was monitored as in Figure 4. The 100 minute timepoint is shown.

    • Prepared by: the Journals Production Manager





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