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Molecular Biology of the Cell: E03-11-0799

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Other genes with interesting expression patterns

Several of the transcripts that were induced in response to several stresses are also cell cycle-regulated (Whitfield et al., 2002). For example, many genes induced in the heat shock response also showed a peak of expression at M-phase in the cell cycle of HeLa cells. Another group of genes, whose transcripts were induced by DTT and menadione in fibroblasts, was regulated in the cell cycle with the highest transcript levels during G1 and S phase. These included genes involved in DNA metabolism (TREX1, TOP1) and cell cycle control (CDKN1B, MNT, MAP2K6). The induction of these genes by stress is not likely to be trivially due to cell cycle arrest at G1/S or prolongation of this phase of the cell cycle because many other S phase-expressed transcripts diminished in response to these stresses.

Several genes showed strong and unique profiles of gene expression. Two of these, the chemotactic and cell growth ligands connective tissue growth factor (CTGF) and insulin-like growth factor binding protein 10 (CYR61), were among the four genes that showed the largest magnitude changes in our experiments. Both of these genes? transcripts increased slightly early after DTT and serum stimulation in both HeLa and fibroblasts, but were then dramatically decreased (more than 100-fold in the case of CYR61). Another gene that responded dramatically to stress was a cytochrome P450 (CYP1B1). CYP1B1 is a monooxygenase with a diverse specificity for aromatic compounds (Goodsell, 2001). Deficiency for this gene leads to a defect in eye development, suggesting a possible role in metabolizing a signal molecule important in development. This gene showed a unique expression pattern: its transcript decreased after heat shock and exposure of HeLa cells to DTT, hydrogen peroxide or confluence, but increased strongly after menadione treatment in both HeLa and fibroblasts and after DTT treatment and confluence in fibroblasts.

Goodsell, D.S. (2001). The molecular perspective: cytochrome P450. Stem Cells 19, 263-264.

Whitfield, M.L., Sherlock, G., Saldanha, A.J., Murray, J.I., Ball, C.A., Alexander, K.E., Matese, J.C., Perou, C.M., Hurt, M.M., Brown, P.O., and Botstein, D. (2002). Identification of genes periodically expressed in the human cell cycle and their expression in tumors. Molecular Biology of the Cell 13, 1977-2000.

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