Supplemental Material

To download and view the large-scale datasets below, click on each link. The files will open in Excel.

This article contains the following supporting material:

• Supplemental Figures 1-2.pdf -
  Supplemental figure 1. MCF-10A ΔRaf-ER and MCF-10A V12Ras cells were cultured in minimal medium either in the presence (4-OHT) or absence (control, V12Ras) of 100μM 4-OHT for 24 hours. Phosphorylation of p42^ERK2/p44^ERK1 was detected using a phosphospecific antibody.
  Supplemental figure 2. Activation of ΔRaf-ER in MCF-10A cells does not induce NF-κB dependent transcription
  MCF-10A ΔRaf-ER cells were transfected with 0.1μg of a NF-κB reporter construct (pBL3xNFkB-CAT) together with 0.5 μg of expression vectors for V12 Ras or empty vector, respectively. In parallel, cells were induced with 4-OHT for 24 or 48 hours. Data represent relative changes in CAT reporter activity and reflect the mean of three independent experiments.
  
• Schulze_et_al_suppl_table_1.XLS
• Schulze_et_al_suppl_Table_2.XLS
• Schulze_et_al_suppl_table_3.XLS
• Schulze_et_al_suppl_table_4.XLS

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