Supplemental Material

This article contains the following supporting material:

• Supplemental Figure.pdf
• Movie1Control.mov (5.12 MB) - Time-lapse phase contrast sequence of a Swiss 3T3 fibroblast subjected to shear flow (τ_w= 9.4 dyn/cm²). Images were captured every minute to document nucleus motion (rotation and translocation). The arrow indicates the direction of flow. Bar, 20 μm.
  
  
• Movie2EGFP_RhoAT19N.mov (5.22 MB) - Time-lapse phase contrast sequence of a Swiss 3T3 fibroblast transfected with EGFP:RhoAT19N subjected to shear flow (τ_w= 9.4 dyn/cm²). Images were captured every minute to document nucleus motion. The arrow indicates the direction of flow. Bar, 20 μm.
  
  
• Movie3EGFP_Rac1T17N.mov (5.15 MB) - Time-lapse phase contrast sequence of a Swiss 3T3 fibroblast transfected with EGFP:Rac1T17N subjected to shear flow (τ_w= 9.4 dyn/cm²). Images were captured every minute to document nucleus motion. The arrow indicates the direction of flow. Bar, 20 μm.
  
  
• Movie4EGFP_Cdc42T17N.mov (5.31 MB) - Time-lapse phase contrast sequence of a Swiss 3T3 fibroblast transfected with EGFP:Cdc42T17N subjected to shear flow (τ_w= 9.4 dyn/cm²). Images were captured every minute to document nucleus motion. The arrow indicates the direction of flow. Bar, 20 μm.
  
  
• Movie5NocodazoleTreated.mov (5.67 MB) - Time-lapse phase contrast sequence of a Swiss 3T3 fibroblast treated with nocodazole subjected to shear flow (τ_w= 9.4 dyn/cm²). Images were captured every minute to document nucleus motion. The arrow indicates the direction of flow. Bar, 20 μm.
  
  
• Movie6Nucleliof5Conditions.mov (9.05 MB) - Montage of nuclei of cells shown in Movie 1-5. In control, EGFP:RhoAT19N, and EGFP:Rac1T17N transfected cells, rigid-body translocation and minor rotation occur. EGFP:Cdc42T17N transfected cell exhibits enhanced rigid-body movement and dramatic concerted NR. In nocodazole-treated fibroblast, the nucleus shape is significantly compromised and nucleoli movements are independent from each other. Bars, 20 μm.
  
  

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