Supplemental Figures

This article contains the following supporting material:

• Supplemental Figure 1.pdf - Electroretinogram recordings. Electroretinograms were recorded from e155/e155 mutants and +/e155 sibling control flies in response to a 4 sec flash of white light. The amplitude of the ON response and the decay of both the ON and OFF responses were similar in the two groups of flies. The time course of the light stimulus is indicated by the horizontal bar at the bottom.
  
• Supplemental Figure 2.pdf - Deletion of the Dlc90F gene. (A) Identification of a deficiency in the Dlc90F region by RFLP analysis. Genomic DNA isolated from adult flies was hybridized with a probe derived from the entire coding region of Dlc90F/I>. Lanes E, B, X: Wild-type genomic DNA digested with EcoRI, BamHI and XbaI, respectively. A single band in each lane demonstrates the probe used is specific. Lanes 1-4: BamHI digests of genomic DNA from (1) F5/TM6B, (2) F5/Df(3R)DG2, (3) F5/TM2 , and (4) Df(3R)DG2/TM2. The wild-type band of 9.5 kb is provided by the balancer chromosomes TM6B or TM2. A 5 kb band results from the insertion of a P element in the Dlc90F gene on the F5 chromosome. The absence of the 9.5 kb band in lane 2 indicates Df(3R)DG2 contains a deletion removing the Dlc90F gene. (B) Imprecise excision of a P element removes Dlc90F. PCR fragments were amplified from flies from line e155 (lane 1) and wild-type (lane 2) using a pair of primers flanking the Dlc90F gene. A deletion leads to a mobility shift of the PCR fragment in the ethidium bromide-stained gel.
  

Prepared by: Journals Production Manager