Supplemental Figures

This article contains the following supporting material:

• Supplemental Figure 1.pdf - Swi6-GFP localization in Sprna1^ts mutants.
  Cultures of indicated cells were incubated at 26°C and then half of cultures were incubated at 37°C for 4 hours. Images of wild-type, Sprna1-86^ts and Sprna1-87^ts were shown as representatives. The localization of Swi6 was detected with GFP-fused Swi6 (gift from Dr. Y. Watanabe, Univ. of Tokyo) that was integrated into the genome. DNA was stained with DAPI (blue). Left columns showed the localization of GFP and right columns showed a merged image with DAPI (blue) at the indicated temperature. Scale bar, 5 μm.
  
  
• Supplemental Figure 2.pdf - Gene silencing activity of Sprna1^ts mutants expressing clr4⁺.
  Sprna1^ts [imr1R::ura4⁺] strains expressing clr4⁺ were spotted. The h⁺975 and h⁺ura4-D18 were used as the control strains of either Ura4⁺ or Ura4^- cells, respectively. The dotted marks of these strains, indicated that they have no plasmids. Each strain was grown to 1.0 x 10⁷ cells/ml in YE5S. Serial dilution (1:5) of the indicated cultures were spotted onto non-selective (N/S), selective (Ura^-), or counterselective (5FOA) plates and incubated at 30°C for five days. The highest density spots contained 1 x 10⁴ cells.

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