Supplemental Figures

This article contains the following supporting material:

• Supplemental Figure 1.pdf - Neither the lack of Amn1 or Swe1 nor the overexpression of CDC5 can suppress cytokinetic and cell growth defects caused by high levels of Dma2.
  A) Cultures of isogenic strains with the indicated genotypes, all carrying multiple copies (m) of the GAL1-DMA2 fusion integrated at the URA3 locus and logarythmically growing in YEPR, were arrested in G1 by &alpha: factor and released in YEPRG at time zero. At different time points cell samples were collected for FACS analysis of DNA contents.
  B) Serial dilutions of the indicated cell cultures were spotted on YEPD (Glu) or YEPRG (Gal) plates and incubated for two days at 25°C.
  
• Supplemental Figure 2 - Lack of Dma1 and Dma2 does not affect Cdc5 protein levels and overexpression of DMA2 does not interfere with separation of sister telomeric sequences.
  A) Wild type (wt) and dma1Δ dma2Δƒ¬ƒ}ƒ¹ƒ® cells grown in YEPD were arrested in G1 by α factor and released from the pheromone arrest in fresh medium. At the indicated time points cells were collected for FACS analysis of DNA contents (not shown) and Western blot analysis of Cdc5-HA3 and Clb2. B: Wild type cells carrying either none (wt) or multiple copies of the GAL1-DMA2 fusion integrated at the URA3 locus, all carrying the tetO/tetR-GFP constructs to monitor sister chromatid separation at subtelomeric regions were grown in YEPR, synchronized in G1 by α factor, and released into YEPRG at time=0. At the indicated times cells were fixed for FACS analysis (not shown) and to follow kinetics of budding and sister chromatid separation.
  

Prepared by: Journals Production Manager