Supplemental Figures

This article contains the following supporting material:

• Supplemental Figures.pdf -
  Supplemental Figure 1: Generation of LAMP-1/LAMP-2 double deficient mice. (A) Breeding scheme to obtain LAMP-double deficient mice after two generations. (B) Statistical distribution of genotypes of postnatal F2 offspring after breeding of LAMP-1/LAMP-2 double heterozygote females with LAMP-1 deficient males. Grey bars indicate the expected numbers/genotype and filled bars show the number of mice obtained per genotype. PCR genotyping did not allow to discriminate between LAMP-2 +/+ and LAMP-2 +/- females. In these cases a question mark indicates the respective allele.
  
  Supplemental Figure 2: Electron microscopical analysis of LAMP-1/LAMP-2 double deficient embryos (A) Anterior root of a spinal nerve. Arrows indicate vacuoles in Schwann cells (SC). Ax, axons in cross and longitudinal sections. (B,C) A neuroepithelial cell with an autophagic vacuole, which is shown in E at higher magnification. Bars, 0.5 μm (A), and 1 μm (B).
  
  Supplemental Figure 3: Altered MPR300 distribution in LAMP-1/LAMP-2 double deficient MEFs. MPR300 immunofluorescence labelling in (A) control, (B) LAMP-1 single deficient, (C) LAMP-2 single deficient and (D) LAMP-1/LAMP-2 double deficient MEFs. Note the more vesicular distribution in double knockout cells. Bar, 20 μm. The images show representative cells, based on experiments with at least two independent cell lines for each genotype.
  
  Supplemental Figure 4: MPR46 and filipin colocalisation in control and LAMP-1/LAMP-2 double deficient MEFs. Double labelling of MPR46 (red) and filipin (green). The labels largely colocalise in control cells (A, yellow) whereas much less colocalisation is seen in LAMP-1/LAMP-2 double deficient cells (B). Bar, 20 μm.
  

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