Characterization of a Nonclathrin Endocytic Pathway: Membrane Cargo and Lipid Requirements
Mol. Biol. Cell Naslavsky et al.
15: 3542
Supplemental Figure
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Supplemental Figure 1.pdf -
Total CD59 is detergent-resistant whereas MHCI is detergent soluble. Untransfected HeLa cells were cooled on ice and incubated 3 min with PBS (control) or with PBS containing 1% TX-100 and then fixed. Total CD59 and MHCI were assessed with FITC-anti-CD59 and Cy3-anti-MHCI antibodies in the presence of saponin.