Supplemental Material

Supplemental Material and Videos

This article contains the following supporting material:

• Supplemental Material 7.pdf - Construction of plasmids used in this study.
  
• Supplemental Material 1.avi - Division of a W303-1A RNT1 cell expressing GFP-Tub3p. Pictures from multiple focal points were taken every 10 minutes and merged to follow cell division and microtubules movement. Time in minutes is indicated on each frame.
  
• Supplemental Material 2.avi - Division of a Δrnt1 cell expressing GFP-Tub3p. Pictures from multiple focal points were taken every 10 minutes and merged to follow cell division and microtubules movement. Time in minutes is indicated on each frame.
  
• Supplemental Material 3.avi - Movement of the GFP-Tub3p tagged microtubule in a Δrnt1 cell failing to divide correctly. Microtubules never extend and the nucleus does not divide. Instead, the nucleus travels from the mother to the daughter cell resulting in an anucleated cell at the end of cytokinesis. Pictures from multiple focal points were taken every 10 minutes and merged to follow cell division and microtubules movement. Time in minutes is indicated on each frame.
  
• Supplemental Material 4.avi - Movement of GFP-Rnt1p through the division of a cell released from α-factor G1 synchronization. Pictures from multiple focal points were taken every 20 minutes and merged to follow cell division and microtubules movement. Time in minutes is indicated on each frame.
  
• Supplemental Material 5.avi - Movement of the GFP-Tub3p tagged microtubule in a Δrnt1 cell expressing GFP-Rnt1p-K45/I from which the fluorescence emitted is negligible compared to that of GFP-Tub3p and cannot be detected with the exposure time used in this experiment. The pre-mitotic spindle shuttles from mother to daughter cell before elongating and dividing. Pictures from multiple focal points were taken every 10 minutes and merged to follow cell division and microtubules movement. Time in minutes is indicated on each frame.
  
• Supplemental Material 6.avi - Movement of the GFP-Tub3p tagged microtubule in a Δrnt1 cell expressing GFP-Rnt1p-D247/R from which the fluorescence emitted is negligible compared to that of GFP-Tub3p and cannot be detected with the exposure time used in this experiment. Pictures from multiple focal points were taken every 10 minutes and merged to follow cell division and microtubules movement. Time in minutes is indicated on each frame.
  

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