Supplemental Figures

This article contains the following supporting material:

• Supplemental Figure 1.pdf - CHC22 localizes to the postsynaptic side of the neuromuscular junction of rat muscle.
  Rat muscle tissue was fixed and embedded in optimal cutting temperature compound for cryo-sectioning. Sections were prepared for immunofluorescence and labelled with a polyclonal antibody to CHC22 (A, C red, D, G green, H, K red) and monoclonal antibodies to the synaptic vesicle protein 2 (SV2) that resides in the nerve terminal (B, C green), CHC17 (E, G red) or nestin (I, K green), and FITC-conjugated BngTx (F, G blue, J, K blue). Nuclei are stained by DAPI blue (C). Each row shows the same field viewed with different filters and the right hand panel shows the merged images. Scale bars in microns.
  
• Supplemental Figure 2.pdf - Intermediate filament proteins desmin and nestin co-localize with CHC22 at early stages of differentiation.
  Rat L8E63 skeletal muscle cells grown on collagen-coated cover slips were induced to differentiate by serum starvation. Cells were then fixed at days indicated after serum withdrawal and processed for immunofluorescence labeling using antibodies to CHC22 (A, C, D, F, G, I, J, L, M and O red) and desmin (B, C, E, F, H and I green) or nestin (K, L, N and O green). Note the early (days 1 and 2) co-localization of CHC22 with both intermediate filament proteins and their subsequent segregation at later stages of differentiation when cells become multinucleated (nuclei stained with DAPI in merged images I, L and O blue). Each row shows the same field viewed with different filters and the right hand panel shows the merged images. Scale bars in microns.
  

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