Supplemental Material

This article contains the following supporting material:

• vid1fig1.mov - Vid1Fig1. Flashing: Cyclic assembly and disassembly of actin around L. monocytogenes. MDCK A10* cell infected with wild-type L. monocytogenes. Paired phase contrast and fluorescence (GFP-actin) time-lapse video. Three cycles of transient actin accumulation occur on this bacterium. Frames were collected at 4 second intervals and are displayed at 15 frames/second. Scale bar is 2 μm. Supplement to Figure 1A.
  
  
• vid2fig1.mov - Flashing: Cyclic assembly and disassembly of actin around L. monocytogenes. MDCK A10* cell infected with wild-type L. monocytogenes. Paired phase contrast and fluorescence (GFP-actin) time-lapse video. Flashing actin is passed among the bacteria. Frames were collected at 4 second intervals and are displayed at 15 frames/second. Scale bar is 2 μm. Supplement to Figure 1B.
  
  
• vid3fig5.mov (3.12 MB) - Flashing occurs on E-cadherin-coated bead phagosomes. MDCK A10* cells with internalized E-cadherin-coated beads. Paired phase contrast and fluorescence (GFP-actin) time-lapse video. Frames were collected at 3 second intervals and are displayed at 20 frames/second. Scale bar is 2 μm. Supplement to Figure 5A.
  
  
• vid5fig7.mov (4.61 MB) - Flashing actin is associated with an unusually long bacterium which deforms the plasma membrane. MDCK A10* cell infected with L. monocytogenes DP-L4032. Paired phase and fluorescence (GFP-actin) time-lapse video. Flashing occurs on the membrane-bound portion of the bacterium. The bacterium is eventually thrust out of the cell. Frames were collected at 3 second intervals and are displayed at 20 frames/second. Scale bar is 2 μm. Supplement to Figure 7A.
  
  
• vid7fig7.mov - Flashing actin dynamics at the edge of a wound in a cell. MDCK A10* cell microinjection needle wound. Paired phase and fluorescence (GFP-actin) time-lapse video. The edge of the wound has flashing actin dynamics. Frames were collected at 5 second intervals and are displayed at 12 frames/second. Scale bar is 2 μm. Supplement to Figure 7C.
  
  
• vid8fig8.mov - Actin comet tails can emerge from flashing objects. MDCK A10* cell infected with L. monocytogenes δactA. Paired phase contrast and fluorescence (GFP-actin) time-lapse video. The bacterium flashes and an actin comet tail emerges from the flashing phagosome. Frames were collected at 3 second intervals and are displayed at 20 frames/second. Scale bar is 2 μm. Supplement to Figure 8A.
  
• video4.mov (2.27 MB) - Entry and flashing of E. coli (invasin). MDCK A10* cell with E. coli (invasin). Paired phase contrast and fluorescence (GFP-actin) time-lapse video. Weak actin accumulation begins after contact between the bacterium and the cell and outlines the presumptive phagocytic cup (3:00?7:00). More intense actin accumulation occurs during flashing (12:20). Frames were collected at 3 second intervals and are displayed at 20 frames/second. Scale bar is 2 μm.
  
  
• video6.mov - Flashing actin can transiently deform vesicles. MDCK A10* cell treated with 50 μM LY 294002. Paired phase contrast and fluorescence (GFP-actin) time-lapse video. Actin accumulates on the vesicle. Vesicle squeezing is accompanied by vesicle movement. Frames were collected at 4 second intervals and are displayed at 15 frames/second. Scale bar is 2 μm.
  
  

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