Supplemental Material

This article contains the following supporting material:

• SupMatmovie1.mov (3.17 MB) - NRK cells treated with 5 μg/ml BFA.
  NRK cells expressing YFP-GBF1 were treated with 5 μg/ml BFA. The first image was taken with the cells in medium lacking BFA, which was then removed and replaced with BFA-containing medium.
  
  
• SupMatmovie2.mov (6.75 MB) - NRK cells treated with 1 μg/ml BFA.
  NRK cells expressing YFP-GBF1 were treated with 1 μg/ml BFA. The first image was taken with the cells in medium lacking BFA, which was then removed and replaced with BFA-containing medium.
  
  
• SupMatmovie3.mov (4.69 MB) - FLIP in NRK cells overexpressing YFP-GBF1.
  NRK cells expressing YFP-GBF1 were subjected to repeated photobleaching (FLIP) of a cytosoplasmic area in untreated NRK cells, with one bleach every 15 seconds.
  
  
• SupMatmovie4.mov (1.86 MB) - FLIP of YFP-GBF1 after addition of 1 μg/ml BFA.
  NRK cells expressing YFP-GBF1 were treated with 1 μg/ml BFA for 30 minutes, then subjected to repeated photobleaching of a cytoplasmic area, with one bleach every 40 seconds.
  
  

Prepared by: Journals Production Manager