Supplemental Material

This article contains the following supporting material:

• SuppMovie1.mov - Wild-type embryos lack DNA segregation defects.
  Fluorescent spinning disc confocal image of a one-cell wild-type embryo carrying a histone2B and β-tubulin::GFP signal.
  
  
• SuppMovie2.mov (3.47 MB) - A one-cell apo-5(or358) embryo without DNA segregation defects.
  Fluorescent spinning disc confocal image of a one-cell apo-5(or358) embryo carrying a histone2B and β-tubulin::GFP signal. Notice that during both metaphase and anaphase, chromosomes do not align as tightly as they do in wild-type embryos, perhaps due to incomplete kinetochore-microtuble attachments in apo-5 embryos as compared to wildtype.
  
  
• SuppMovie3.mov (8.67 MB) - A one-cell apo-5(or358); mdf-1(RNAi) embryo with severe DNA bridges.
  Fluorescent spinning disc confocal image of a one-cell apo-5(or358); mdf-1(RNAi) embryo expressing histone2B::GFP and β-tubulin::GFP fusion proteins. Notice the lack of alignment of chromosomes during presumed metaphase and as the chromosomal masses start to be pulled apart. Severe DNA bridges persist as cytokinesis ensues.
  
  

Prepared by: Journals Production Manager