Supplemental Material

This article contains the following supporting material:

• movie1.mov (1.88 MB) - Rab4+FcRn+ vesicles entering and leaving a sorting endosome. Cells were cotransfected with Rab4-YFP (red) and FcRn-CFP (green) and imaged using dual color epifluorescence. Movie plays at four times the acquisition speed. Movie 1 corresponds to Figure 3A.
  
  
• movie2.mov - A Rab11+FcRn+ tubule leaving a sorting endosome. Cells were co-transfected with Rab11-YFP (red) and FcRn-CFP (green) and imaged using dual color epifluorescence. Movie plays at twice the acquisition speed. Movie 2 corresponds to Figure 3B.
  
  
• movie3.mov - A Rab11+FcRn+ tubule merging with a sorting endosome. Cells were cotransfected with Rab11-YFP (red) and FcRn-CFP (green) and imaged using dual color epifluorescence. Movie plays at twice the acquisition speed. Movie 3 corresponds to Figure 3C.
  
  
• movie4.mov - Exocytic event involving Rab11 and FcRn fusion with the plasma membrane. Cells were co-transfected with Rab11-YFP (red) and FcRn-CFP (green) and imaged using dual color TIRFM. Movie plays at twice the acquisition speed. Movie 4 corresponds to Figure 4A.
  
  
• movie5.mov - Exocytic event showing that only FcRn, but not Rab4, fuse with the plasma membrane. Cells were co-transfected with FcRn-YFP (red) and Rab4-CFP (green) and imaged using dual color TIRFM. Movie plays at twice the acquisition speed. Movie 5 corresponds to Figure 4B.
  
  
• movie6.mov (1.14 MB) - A Rab4+Rab11+ vesicle leaving a sorting endosome and then separating into Rab4+ and Rab11+ compartments. Cells were co-transfected with Rab4-CFP (green) and Rab11-YFP (red) and imaged using dual color epifluorescence. Movie plays at twice the acquisition speed. Movie 6 corresponds to Figure 6A.
  
  
• movie7.mov - A Rab4+Rab11+ vesicle separating into Rab4+ and Rab11+ compartments. Cells were co-transfected with Rab4-CFP (green) and Rab11-YFP (red) and imaged using dual color epifluorescence. Movie plays at twice the acquisition speed. Movie 7 corresponds to Figure 6C.
  
  
• movie8.mov - A Rab4+FcRn+ vesicle separating into Rab4+ and FcRn+ compartments. Cells were co-transfected with Rab4-YFP (red) and FcRn-CFP (green) and imaged using dual color epifluorescence. Movie plays at twice the acquisition speed. Movie 8 corresponds to Figure 7A.
  

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