Supplemental Material

This article contains the following supporting material:

• Movie_1_ase1_MT_MBC.mov (1.74 MB) - Wild-type (PT47) cells expressing GFP-tub1p. Cells are in an MBC perfusion chamber. Movie shows maximum projections of 3D stacks. Total frames = 50. Time interval between frames = 15 sec. Total elapsed time: 12.5 min. MBC perfusion at frame 10. MBC washout with fresh media at frame 30.
  
  
• Movie_1_wt_MT_MBC.mov (2.19 MB) - Ase1Δ (PT311) cells expressing GFP-tub1p. Cells are in an MBC perfusion chamber. Movie shows maximum projections of 3D stacks. Total frames = 50. Time interval between frames = 15 sec. Total elapsed time: 12.5 min. 50 μg/ml MBC perfusion at frame 10. MBC washout with fresh media at frame 30.
  
  
• Movie_2_ase1_MT.mov (6.44 MB) - Wild-type (PT65) cells expressing Nup107p-GFP and GFP-tub1p. Movie shows maximum projections of 3D stacks. Total frames = 390. Time interval between frames = 20 sec. Total elapsed time: 130 min.
  
  
• Movie_2_wt_MT.mov (8.53 MB) - Ase1Δ (PT335) cells expressing Nup107p-GFP and GFP-tub1p. Movie shows maximum projections of 3D stacks. Total frames = 300. Time interval between frames = 20 sec. Total elapsed time: 100 min.
  
  
• Movie_3_ase1_sad1GFP.mov (3.20 MB) - Wild-type (PT104) cells expressing GFP-sad1p. Movie shows single optical sections. Total frames = 360. Time interval between frames = 5 sec. Total elapsed time: 30 min.
  
  
• Movie_3_wt_sad1GFP.mov (4.57 MB) - Ase1Δ (PT337) cells expressing GFP-sad1p. Movie shows single optical sections. Total frames = 320. Time interval between frames = 5 sec. Total elapsed time: 27 min.
  
  
• Movie_4_ase1GFP.mov - Wild-type (PT306) cell expressing ase1p-GFP. Movie shows maximum projections of 3D stacks. Total frames = 60. Time interval between frames = 1 min. Total elapsed time: 60 min. Interphase to mitosis transition occurs at frames 20-25.
  

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