Supplemental Movies

This article contains the following supporting material:

• Figure 7B:GFP-GIPC1 movement towards the cell body in CHO cells - Movement of GFP-GIPC1 towards the cell body in cellular extensions from the advancing(A) and trailing(T) edge of a CHO cell
• Figure 7C:Coordinate Movement of CFP-myosin VI/YFP-GIPC1 Complexes in 293 cells - Movement of CFP-myosin VI (upper left, green) panel and YFP-GIPC1 (upper right panel, red) in live CHO cells. Complexes of CFP-myosin VI/YFP-GIPC1 (arrows)can be observed to move together toward the cell body (merged view lower left panel) within the cellular extentions
• Figure 8A:Effects of Colchicine on GFP-GIPC1 Movement in CHO Cells - Disruption of microtubules by colchicine (added at 12 min) does not impede the movement of GFP-GIPC1 within cellular extensions of CHO cells
• Figure 8B:Effects of Nocodazole on GFP-GIPC1 Movement in CHO Cells - Disruption of microtubules by nocodazole (added at 13 min) does not impede the movement of GFP-GIPC1 within cellular extensions of CHO cells
• Figure 8C:Effects of Cytochalasin D on GFP-GIPC1 Movement in CHO Cells - Disruption of F-actin by cytochalasin D (added at 6 min) blocks movement of GFP-GIPC1 within cellular extensions of CHO cell
• Figure 8D:Effects of Latrunculin on GFP-GIPC1 Movement in CHO Cells - Disruption of F-actin by latrunculin (added at 13 min) blocks movement of GFP-GIPC1 within cellular extensions of CHO cells
• Figure 8E Effects of CFP-myosin VI(955-1254) Expression on YFP-GIPC1 - CFP-myosin VI(955-1254) blocks movement of YFP-GIPC1 by preventing its interaction with native myosin VI. The distance expected for normal GIPC1 particle movement in the absence of mutant myosin VI during the 10 min collection time of the movie is represented by the length of the white arrow.
• Figure 9A:Distribution of GFP-GLUT1 in Polarized MDCK Cells - A 3-D reconstruction of GFP-GIPC1 expressed in a living polarized MDCK cell. GFP-GLUT1 two days after transfection is properly excluded from the apical membrane and expressed only in the basolateral membrane of the cell. GFP-GLUT1 is not concentrated in large intracellular vesicles to any detectable extent
• Figure 9B:Distribution of GFP-GLUT1Δ4 in Polarized MDCK Cells - Two days after transfection low concentrations of GFP-GLUT1 Δ4 are present in the plasma membrane, while a high level of GFP-GLUT1 Δ4 is evident in large cytosolic vesicles