Targeting of Protein Kinase C- during Fc Receptor-dependent Phagocytosis Requires the C1B Domain and Phospholipase C-1

Supplemental Material

This article contains the following supporting material:

• Video 1 - PKC-ε localizes to targets during IgG-mediated phagocytosis: real-time imaging. RAW cells were transiently transfected with GFP-conjugated PKC-ε. BIgG-targets were added and images were collected every 10 s for 10 min. PKC-ε accumulation is transient, concentrating upon target binding and lost after internalization.
• Video 2 - εΔC1A localizes to targets during IgG-mediated phagocytosis: real-time imaging. RAW cells were transiently transfected with GFP-conjugated εΔC1A. BIgG-targets were added and images were collected every 10 s for 10 min. εΔC1A accumulation is transient, concentrating upon target binding and lost after internalization.
• Video 3 - εΔC1B does not localize to targets during IgG-mediated phagocytosis: real-time imaging. RAW cells were transiently transfected with GFP-conjugated εΔC1B. BIgG-targets were added and images were collected every 10 s for 10 min. εΔC1B does not concentrate around phagosomes.
• Video 4 - PLD2 localizes to targets during IgG-mediated phagocytosis: real-time imaging. RAW cells were transiently transfected with GFP-conjugated PLD2, BIgG-targets were added and images were collected every 10 s for 10 min. PLD2 accumulates around nascent phagosomes and dissipates after internalization, similar to PKC-ε.