Supplemental Videos

This article contains the following supporting material:

• Video 1 - Mature-human DC nucleotransfected with αL-GFP and preloaded with SEB were adhered to fibronectin coated coverslips and mounted in chambers. S3 T cells labelled in red with the probe CM-TMR were added to the chambers, and individual live DC was monitored using time-lapse confocal microscopy. Images were taken at 30s intervals. Video represents the superimposed images from GFP fluorescent signal (green), the DIC image and the red fluorescence signal from T cells.
• Video 2 - Mature-human DC nucleotransfected with CD44-GFP and preloaded with SEB were adhered to fibronectin coated coverslips and mounted in chambers. S3 T cells labelled in red with the probe CM-TMR were added to the chambers, and individual live DC was monitored using time-lapse confocal microscopy. Images were taken at 30s intervals. Video represents the superimposed images from GFP fluorescent signal (green), the DIC image and the red fluorescence signal from T cells.
• Video 3 - Mature-human DC nucleotransfected with MHC class-II-GFP and preloaded with SEB were adhered to fibronectin coated coverslips and mounted in chambers. S3 T cells labelled in red with the probe CM-TMR were added to the chambers, and individual live DC was monitored using time-lapse confocal microscopy. Images were taken at 30s intervals. Video represents the superimposed images from GFP fluorescent signal (green), the DIC image and the red fluorescence signal from T cells.

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