Supplemental Videos

This article contains the following supporting material:

• Video 1 - Early and late rRNA processing proteins colocalize at the periphery of chromosomes during anaphase. Localization of fibrillarin, DNA, Bop1 and B23 (from left to right) are shown in the same fixed cell by animating the acquisition stacks (56 focal planes) that go from top to bottom.
• Video 2 - Compared dynamics during telophase of early (fibrillarin) and late (B23) rRNA processing proteins show unique sites of PNB formation and differential sorting of early and late processing machineries. Acquisition frequency: 30 sec; 174 red and 174 green stacks; thickness 15 ?m. GFP-fibrillarin and DsRed-B23 were expressed in the same living cell. Their dynamics visualized by GFP and DsRed show that these two proteins are recruited in the same PNBs but are addressed in a sequential order to the incipient nucleoli at the time of nucleolar recruitment.
• Video 3 - Compared dynamics of Nop52 and B23, two late rRNA processing proteins. Acquisition frequency: 30 sec; 120 red and 120 green stacks; thickness 15 ?m. GFP-Nop52 and DsRed-B23 were expressed in the same living cell. Comparison of the dynamics of both proteins shows that they are present in the same PNBs and simultaneously addressed to the nucleoli at the time of nucleolar recruitment.

Prepared by: Journals Production Manager