Supplemental Material

This article contains the following supporting material:

• Movie 1 - YFP-VSVG transport from the ER to the Golgi at 32°C in CHO cells.
  CHO cells were transfected with YFP-VSVG and blocked at 39.5°C overnight. Cells were then moved to the stage of an inverted wide field fluorescent microscope controlled at 32°C. Images were taken every 30 s and total 61 frames were recorded (30 min real time).

• Movie 2 - YFP-VSVG transport from the ER to the Golgi at 32°C in CHO cells treated with statin.
  CHO cells were prepared identically as in Movie 1 except that, before moving to 32 °C, cells were treated with lovastatin (40 μM) for 1 h. Images were again taken every 30 s and 30 min total in real time.

• Movie 3 - GFP-εCOP movements in CHO cells.
  CHO cells stably expressing GFP-εCOP were imaged at 32 °C. Images were taken every 20 s and 30 min total in real time (91 frames).

• Movie 4 - GFP-εCOP movements in CHO cells treated with statin.
  CHO cells stably expressing GFP-εCOP were treated with lovastatin (40 μM) for 1 h and then imaged at 32 °C. Images were again taken every 20 s and 30 min total in real time (91 frames).