Supplemental Material

This article contains the following supporting material:

• Figure S1-1 - (A) Data sets in Figure 2B were detrended by subtracting the 24 hr running average from the raw data. For accurate comparison, thin lines show the curve for “SV40-luc”. (B) In Per2 (-45~-6)-SV40-luc and Per2 (-25~+15)-SV40-luc, the maximum differences between the smoothed curves for each cycle (the peak and the trough) were used to calculate the amplitude of each cycle. Data represent the mean ± SEM of triplicate samples. (C) The Per2 (-45~-6)-SV40-luc/Per2 (-25~+15)-SV40-luc amplitude ratio was obtained by dividing the Per2 (-45~-6)-SV40-luc wave amplitude by the Per2 (-25~+15)-SV40-luc wave amplitude in each cycle. (D) Data sets in Figure 2D were detrended by subtracting the 24 hr running average. Thin lines show the curve for “SV40-luc” . (E) In Per2 (-25~-6)-SV40-luc and Per2 (-30~-11)-SV40-luc, the maximum differences in each cycle were used to calculate the amplitude. Data represent the mean ± SEM of triplicate samples. (F) The Per2 (-25~-6)-SV40-luc/Per2 (-30~-11)-SV40-luc amplitude ratio was obtained by dividing the Per2 (-25~-6)-SV40-luc wave amplitude by the Per2 (-30~-11)-SV40-luc wave amplitude in each cycle. (G) The merge of Per2 (-25~-6)-SV40-luc (Supplemental Fig. 1D) and Per2 (-45~-6)-SV40-luc (Supplemental Fig. 1A) is shown.
• Figure S1-2
• Figure S2-1 - (A) Data sets in Figure 3A were detrended by subtracting the 24 hr running average. For accurate comparison, thin lines show the curve for “mPer2-luc (-105)” . (B) In each wild-type or deletion construct, the period was obtained from regression analysis of a circadian marker (trough). Data represent the mean ± SEM of triplicate samples. (C) The maximum differences between the smoothed curves for each cycle (the peak and the trough) were used to calculate the amplitude of each cycle. Thin lines show the curve for “mPer2-luc (-105)” . Data represent the mean ± SEM of triplicate samples. (D) The initial amplitude in (C) was set to 100.
• Figure S2-2
• Figure S3 - The effect of deletion mutation on Per2 basal transcription was evaluated by using a luciferase assay. The basal transcriptional activity of mPer2-luc (-105) was set to 1. Data represent the mean±SEM of triplicate samples.