Supplemental Figures

This article contains the following supporting material:

• Figure S1 - Effect of mec1-85 mutation on cell viability after MMS treatment. Viability was determined after treatment of various concentrations of MMS for 30 min. Strains used were wild-type (KSC1560), mec1Δ (KSC1561), and mec1-85 (KSC1641) cells.
• Figure S2 - Degradation of the HO-induced DSB ends. Wild-type (KSC1560) and mec1-85 (KSC1641) cells carrying YCpA-GAL-HO were grown in sucrose and treated with nocodazole. After arrest at G2/M, the culture was incubated with galactose to induce HO expression. Purified DNAs were fixed to a membrane and hybridized with oligonucleotide probes, each complementary to 5’ to 3’- or 3’ to 5’-degrading strand.