Supplemental Movies

This article contains the following supporting material:

• Movie M1 - First two cleavages in a wild type embryo.
  
  Anterior end of embryo is to the left. Nuclear divisions are followed by histone::GFP reporter. Note the anterior cell shape and gap between the eggshell and AB cell (arrow). Note the degree of constriction at the cleavage plane and the maintenance of cell membrane boundaries.
• Movie M2 - First two cleavages in a ppXyl-T RNAi embryo.
  
  Anterior end of embryo is to the left. Nuclear divisions are followed by histone::GFP reporter. Arrows show membrane cleavage plane, which collapses after karyokinesis is complete. The embryo also looses shape and flattens against the eggshell. After the second cleavage, the cell membrane separating all four nuclei disappears.
• Movie M3 - Z-focal scan of an anti-Gal-T2 antibody-stained embryo.
  
  The focal scan of an 8-cell embryo (from Fig. 5A) shows how the anti-Gal-T2 scFv antibody stains the Golgi apparatus in a perinuclear pattern, with the Golgi apparatus wrapping around the nucleus.