Supplemental Material

This article contains the following supporting material:

• Figure 1 - barA mutants are resistant to the effects of HPLC-purified HSAF. Top row; purified HSAF inhibits the growth of wildtype hyphae in a dose-dependent manner. Bottom row, barA1 mutants display equal growth at all tested doses of purified HSAF. Indicated doses represent dilutions of HPKLC-purified HSAF.

• Figure 2 - Alignment of Lag1 homologues. A multiple alignment was generated using ClustalW (MacVector v7.0). Identities are outlined in dark shaded boxes and similarities in light shaded boxes. Protein sequences used for the alignment include: An BarA (A. nidulans BarA , accession number: EAA60493); An LagA (A. nidulans Lag1 homologue, accession number: EAA64170); Sc Lag1( S. cerevisiae Lag1p, accession number: UO8133); Sc Lac1( S. cerevisiae Lac1p, accession number: NP_012917); and Hs Lag1 (Homo sapiens Lag1p homologue, accession number: BC032565 ). * indicates locations of nonsense mutations in barA mutants. The circled * indicates the barA1 mutation.

• Figure 3 - Alignments of BasA homologues. A multiple alignment was generated using ClustalW (MacVector v7.0). Identities are outlined in dark shaded boxes and similarities in light shaded boxes. Protein sequences used for the alignment include: An BasA (A. nidulans BasA, accession number: XM_404777); Sc Sur2 (S. cerevisiae Sur2p, accession number: UO7171); Hs SC4MOL (H. sapiens methyl oxidase-like protein, accession number: NM_006745). * indicates location of the mutation in basA1 mutant 8-145.

• Figure 4 - Growth defects of lagA mutants. (A,B) Conidia from lagA deletion mutant ASL11 (A) and wildtype strain A28 (B) were germinated in YGV media for 12 h. (C,D) The lagA deletion mutant ASL11 (C) and wild type A28 (D) were incubated on MAG plates at 28°C for 10 days. Bar, 3 μm.