Supplemental Material

This article contains the following supporting material:

• Video 1 - Microtubule bending in a yeast-like cell of Ustilago maydis. Due to repressed GFP-αtubulin expression microtubules contain speckles that serve as landmarks on the moving microtubule. Total observation time is 30 sec (2 frames/sec).
• Video 2 - Microtubules in a control strain expressing GFP-alpha tubulin. Microtubules undergo lateral motility and are often bundled. The bundles also undergo dynamic rearrangement. Total observation time is 30 sec (2 frames/sec).
• Video 3 - Microtubules in a strain that was deleted for kin1, the conventional kinesin gene in Ustilago maydis. Cells contain slightly less bundles. Total observation time is 30 sec (2 frames/sec).
• Video 4 - Microtubules in a strain that overexpresses Kin1, which leads to increased microtubule bending. Total observation time is 30 sec (2 frames/sec).
• Video 5 - Microtubules in a strain that expresses a Kin1 mutant protein that contains a rigor mutation in its motor domain and confers microtubule bundling. Total observation time is 30 sec (2 frames/sec).
• Video 6 - Microtubules in a strain that expresses a Kin3-Kin1 chimerical protein that contains a rigor mutation in the Kin3 motor head and a Kin1 tail. Note that Kin3 is a Kif1A-like kinesin and the Kin3 head should not specifically interact with the tail. Total observation time is 30 sec (2 frames/sec).
• Video 7 - Microtubules in a strain that expresses the Kin3rigor-Kin1tail chimerical protein that is deleted for the third coiled-coil and the globular tail (DeltaC3G2). Total observation time is 30 sec (2 frames/sec).