Induction of Dedifferentiation, Genomewide Transcriptional Programming, and Epigenetic Reprogramming by Extracts of Carcinoma and Embryonic Stem Cells
Mol. Biol. Cell Taranger et al.
16: 5719
Supplemental Material
This article contains the following supporting material:
Figure S1 -
Distribution into functional classes of genes up- and downregulated at a >3-fold difference level in NCCIT cells relative to 293T cells.
Figure S2 -
Induction of differentiation of retinoic acid-treated 293T and NCCIT cells. (A) Neurogenic differentiation was induced as in Figure 7D. (B) Anti-NeuN and (C) anti-NF200) immunofluorescence analysis of 293T and NCCIT cells after retinoic acid exposure and plating as in Figure 7D. (D) Oil-Red-O staining after adipogenic differentiation as in Figure 8A. (E) Alzarin red staining following osteogenic differentiation as in Figure 8B. Arrows point to mineralized nodules. (F) Morphology of 293T and NCCIT cells after induction of endothelial differentiation on methylcellulose. Bars, (A-D) 40 μm, (E,F) 200 μm,. Insets, DNA labeled with Hoechst 33342.