Supplemental Material

This article contains the following supporting material:

• Movie 1A - Vero cells expressing GFP-KDEL-R, duration 50 secs.
• Movie 1B - Vero cells expressing GFP-KDEL-R, duration 50 secs.
• Movie 1D - Vero cells expressing GFP-ERGIC-53, duration 38 secs.
• Movie 3A - HeLa cell expressing soluble GFP, duration 38 secs.
• Movie 3B - HeLa cell expressing soluble GFP, duration 50 secs.
• Movie 5A - HeLa cells stably expressing p24-YFP at 37°C, duration 50 secs.
• Movie 5B - HeLa cells stably expressing p24-YFP at 37°C following incubation at 15°C, duration 38 secs.
• Movie 6C - HeLa cells stably expressing p24-YFP in the presence of nocodazole, duration 38 secs.
• Movie 6D - HeLa cells stably expressing p24-YFP following nocodazole wash-out, duration 50 secs.
• Movie 7A - HeLa cell stably expressing p24-YFP following microinjection with p24 tail peptide, duration 38 secs.
• Movie 7B - HeLa cell stably expressing p24-YFP following microinjection with p23 tail peptide, duration 38 secs.
• Movie 7C - HeLa cell stably expressing p24-YFP following microinjection with p24 lumenal peptide, duration 38 secs.
• Movie 7D - HeLa cell stably expressing p24-YFP following microinjection with Rab-GDI, duration 38 secs.
• Movie 7E - HeLa cell stably expressing p24-YFP following microinjection with GTPγS, duration 38 secs.