Supplemental Material

This article contains the following supporting material:

• Movie 1 - Rap1 activation monitored by FRET imaging of vascular endothelial cells moving spontaneously (elapsed time is indicated as hour : min).
• Movie 2 - Video image for EGFP-MAGI-1. Note that EGFP-tagged MAGI-1 localizes to cell-cell contacts.
• Movie 3 - Video image for EGFP-MAGI-1 lacking PDZ domain 5. Note that MAGI-1 lacking PDZ domain 5 no longer localizes to cell-cell contacts
• Movie 4 - FRET video image for Rap1 activation in HUVECs overexpressing MAGI-1 PDZ domain 5. Note that Rap1 activation is inhibited at the cell-cell contacts.
• Movie 5 - FRET video image for Rap1 activation in MAGI-1–depleted HUVECs. Note that Rap1 activation is inhibited at the cell-cell contacts in MAGI-1–depleted cells.
• Figures 1 and 2