Supplemental Material

This article contains the following supporting material:

• Figure 1 - Suppression of Numb by siRNA in mature hippocampal neurons.

  (A) Quantitative analysis for the expression level of Numb in older neurons. DIV21 neurons were transfected with siRNA along with GST and fixed 4 d later. Fluorescence intensity of the cell body was measured to confirm the efficiency of Numb siRNAs. (B, C) Effects of Numb knockdown on spine density (B) and spine length (C) in mature neurons. Under the same conditions those in (A), spine morphology was analyzed. The data are means ± SE of pooled samples from at least three independent experiments. (D) Colocalization of Numb and intersectin at clathrin-coated structures in Hela cells. Hela cells were stained with anti-Numb antibody (green), anti-intersectin antibody (red), and phalloidin (blue). Enlarged views of the boxed area are shown on the bottom.