Supplementary Material

This article contains the following supporting material:

• Video 1 - Video1 corresponds to Figure 1. Volume-rendering View of Centromeres in the Nuclei of Guard Cells. Centromeres were labeled with HTR12-Venus in green, and chromatin was labeled with H2B(HTB1)-CFP in red. All twenty centromeres in a pair of guard cells were observed to localize at the nuclear periphery.
• Video 2 - Video2 corresponds to Figure 4A. Dynamic Views of Centromeres in a Root Meristematic Cell in Interphase. The centromeres are labeled with HTR12-GFP. Time-lapse 2-D images (single section) up to 75 minutes were assembled into the video.
• Video 3 - Video3 corresponds to Figure 5A. Dynamic views of Centromere Movements through Mitosis. The centromeres were labeled with HTR12-GFP in green.
• Video 4 - Video4 corresponds to Supplementary Figure S3A. Dynamic views of Centromere Movements through Mitosis. The centromeres were labeled with HTR12-GFP in green.
• Figure S1 - Twenty image sections with 0.2 μm interval through nuclei of guard cells. The centromeres are labeled with HTR12-Venus in green, and chromatin lablled with HTB1-CFP in magenta. Centromeres in the nuclei, which were defined as brightest point at the local foci along the z-axis, are highlighted with arrows. Bar = 5 μm.
• Figure S2 - Seven image sections with 0.6 μm interval through nuclei of mother cell and its daughter cells. The centromeres are labeled with HTR12-GFP. Due to the lower resolution in the z-axis, some centromeres are stretched along the z-axis. Centromeres in the nuclei, which were defined as brightest point at the local foci along the z-axis, are highlighted with numbered arrows. Bar = 5 μm.
• Figure S3A - Tracking Centromeres through Mitosis in 3-D (see also Supplementary Video4). (A) Projection images of nuclei of a mitotic cell at elapse time of 0 min, 6 min, 19 min 30 s, 36 min 45 s and 48 min. Centromeres are highlighted with numbered arrows at each time point. Cycled spots are projected signals from adjacent cells. Bar = 5 μm.
• Figure S3B - (B) Diagram of positions of centromeres in nuclei of mother cell and two daughter cells. Z-axis is directed to the bottom of the nuclei. Centromeres in the top sections of the nuclei are represented by numbered dark gray-scaled dots, and centromeres in the bottom sections of the nuclei are represented by numbered light gray-scaled dots.