Supplemental Material

This article contains the following supporting material:

• Figure 1 - Co-immunoprecipitations from CHO-eNOS cells infected with SFV-HA-NOSTRIN_242-506 using anti-eNOS. Caveolin-1 peptides cav_82-101 (scaffolding domain), cav_61-81 or unrelated peptide (RLC24) were added to the lysates as indicated. Immunoblots were probed for NOSTRIN (anti-HA) and eNOS.

• Figure 2 - Caveolin-1 and eNOS accumulate in the perinuclear region of cells co-expressing eNOS, NOSTRIN and mutant dynamin. CHO-eNOS cells were transfected with dynamin-K44A-GFP (dyn-K44A) in the absence (E-H) or presence of co-transfected NOSTRIN (A-D, I-L). (A-D) NOSTRIN and dyn-K44A co-localize. (E-H) Expression of dyn-K44A alone does not alter localization of caveolin or eNOS, except for a slight change in Golgi morphology. (I-L) eNOS and caveolin appear to be stuck in the perinuclear region in cells expressing NOSTRIN and dyn-K44A. Bars 10 μm.