Supplemental Material

This article contains the following supporting material:

• Table 1
• Figure 1 - Absence of functional interaction between Cwp1p and the proteins involved in bud-site selection. (A-C) Normal axial and bipolar budding patterns of haploid and diploid cwp1Δ strains. Cells of strains LSY252 (A) and LSY265 (B) growing exponentially at 30°C were stained with CFW to reveal budding patterns. (C) The budding patterns of LSY265 and the wild-type control strain YEF473 grown at 30°C were compared quantitatively (Schenkman et al., 2002) by scoring the position of each bud scar as distal pole (d), proximal pole (p), or equatorial (e) on 100 cells with one bud scar, 100 cells with 2 bud scars, 100 cells with three bud scars, and 100 cells with four bud scars. (D) Normal localization of Bud9p in a cwp1Δ strain. Strain LSY265 was transformed with plasmid YEpGFP*-BUD9 (Schenkman et al., 2002), grown to exponential phase at 30°C in selective medium, and examined for the localization of GFP-Bud9p. (E-I) Normal localization of Cwp1p in strains lacking proteins essential for normal budding patterns. Strains YHH615 (E), YHH415 (F), AM476 (G), AM273 (H), and AM503 (I) were transformed with plasmid pGS-GFP-CWP1-low, grown to exponential phase, and examined for the localization of GFP-Cwp1p. Long arrows, birth scars; short arrows, GFP-Bud9p at the daughter sides of septa.